Gcnt2 (NM_023887) Mouse Tagged ORF Clone

CAT#: MG206294

Gcnt2 (GFP-tagged) - Mouse glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (Gcnt2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gcnt2
• Synonyms: 5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206294 representing NM_023887
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCTTGGAAGTACAGTCTTTTCAGCCTATCTCTCATTGCTGCCTTGATGCTTATGTTTATGTACG
  ATAGAAAATTATGGAAGAACTATCATTTCCCGAGGGCGGTTTCCAATATTTCAGTCTTAGCGGAAGTCTG
  CTTGCAGATGTTCAGTGGGGAGAGTTTTTATACAGCGGACAGCGCACGGAAAACCACCCTGGAAAACTTC
  ACCTGCCCCGAGTACAAGATTCAAAACCACTACATAACAGAGACTCTCTCTGAAGAAGAAGCCCGCTTCC
  CTCTGGCTTTCACACTTACTATCCACAAAGATTATGACACTTTCGAGAGACTCTTCAGGGCCATTTACAT
  GCCCCAGAACGTCTACTGTGTGCACGTGGATAGTAAGGCGACAGACACCTTCAAAGAAGAGGTGCGGCAG
  TTACTAAGCTGTTTCCCCAATGCCTTCCTGGCCTCTAGGATGGAGCCGGTGGTCTATGGTGGCTTCTCCC
  GGCTCCAAGCTGATCTGAACTGCATGAAAGATCTGGTGGCCTCCAAGATCCCCTGGAAATATGTCCTCAA
  CACCTGCGGGCAGGACTTTCCTCTGAAAACCAACAAAGAAATAGTTCAGTACCTGAAACGGTTTATTGGG
  AAGAACCTCACCCCAGGGGTGCTGCCTCCTGCACATGCAGTTGGAAGAACCAAGTACGTCCACCAGGAGC
  TGTTAGACCATAAAAATCCCTACGTGCACAATACAGCAAGATTAAAAGCTCCCCCACCTCACAACCTGAC
  CATTTACTTTGGCACTGCTTATGTGGCTCTCACACGGGAATTTGCTAACTTTGTCCTCAAAGACCAGCGT
  TCAGTAGACTTAATCTCCTGGTCCAAGGACACGTATAGTCCTGATGAGCACTTCTGGGTGACACTCAATA
  GGATCCCTGGAGTCCCTGGCTCCATGCCACCAAACGCATCCTGGACGGGTAACCTCAGAGCTGTGAAGTG
  GATGGACATGGAAGCGAAGCATGGAGGCTGCCATGGTCACTACGTCCATGGCATTTGTATCTATGGAAAC
  GGAGACTTGCAGTGGCTGATTAATTCGCAAAGCCTGTTTGCTAACAAATTTGAACTCAACACATACCCTC
  TTACCGTGGAATGCCTGGAACTGAGGCTTCGAGAAAGAACACTCAACCAGAGTGAGATCGCCATACAGCC
  GAGCTGGTATTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206294 representing NM_023887
  Red=Cloning site Green=Tags(s)
  
  MGSWKYSLFSLSLIAALMLMFMYDRKLWKNYHFPRAVSNISVLAEVCLQMFSGESFYTADSARKTTLENF
  TCPEYKIQNHYITETLSEEEARFPLAFTLTIHKDYDTFERLFRAIYMPQNVYCVHVDSKATDTFKEEVRQ
  LLSCFPNAFLASRMEPVVYGGFSRLQADLNCMKDLVASKIPWKYVLNTCGQDFPLKTNKEIVQYLKRFIG
  KNLTPGVLPPAHAVGRTKYVHQELLDHKNPYVHNTARLKAPPPHNLTIYFGTAYVALTREFANFVLKDQR
  SVDLISWSKDTYSPDEHFWVTLNRIPGVPGSMPPNASWTGNLRAVKWMDMEAKHGGCHGHYVHGICIYGN
  GDLQWLINSQSLFANKFELNTYPLTVECLELRLRERTLNQSEIAIQPSWYF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023887
• ORF Size: 1203 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_023887.3, NP_076376.3
• RefSeq Size: 3995
• RefSeq ORF: 1206
• Locus ID: 14538
• Gene Summary: Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells. [UniProtKB/Swiss-Prot Function]