P4ha3 (BC082538) Mouse Tagged ORF Clone

CAT#: MG206351

P4ha3 (GFP-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III (cDNA clone

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: P4ha3
• Synonyms: D930031A02Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206351 representing BC082538
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTGCAGGATGTGTACATGCTCAACGTGAAGGGCTTGGCTCGAGGCGTCTTCCAGAGGGTCACGG
  GCTCCTCCATCACCGACCTGTACAGTCCCAGGCAGCTCTTCTCTCTCACAGCGGATGACTGCTTCCAAGT
  TGGCAAGGTGGCCTATGACACAGGGGATTACTACCATGCCATCCCATGGCTGGAGGAAGCTGTCAGTCTC
  TTCCGGAGAGCCCACGGCGAGTGGAAGACAGAGGACGAGGCCAGTCTGGAAGACGCCCTGGATTACTTGG
  CCTTTGCCTGTTTCCAGGTAGGAAATGTTTCATGTGCCCTCAGCCTCTCTCGAGAGTTCCTTGTCTACAG
  CCCAGACAATAAGAGGATGGCCAGGAATGTGTTGAAATATGAAAGGCTCTTGGCTGAGAATGGTCACCAG
  ATGGCGGCTGAGACTGCAATTCAGAGGCCCAACGTACCCCACCTGCAGACCAGGGACACCTACGAGGGGC
  TATGCCAGACCCTGGGCTCCCAGCCAACACACTACCAAATCCCCAGCCTCTACTGCTCCTATGAGACCAA
  TTCCAGCCCCTACCTACTGCTCCAGCCTGCCCGAAAGGAGGTTGTCCACCTACGGCCGCTCATAGCTCTC
  TACCATGACTTTGTCAGTGACGAGGAAGCTCAGAAAATCCGAGAGCTTGCAGAACCGTGGCTCCAGAGAT
  CAGTGGTGGCTTCGGGGGAAAAGCAGTTGCAAGTGGAGTACCGCATCAGCAAAAGTGCCTGGCTAAAGGA
  CACGGTTGATCCAATGCTGGTGACCCTTGACCACCGCATTGCGGCCCTCACAGGCCTTGACATCCAGCCT
  CCCTACGCAGAGTATCTCCAGGTGGTGAACTATGGAATCGGAGGGCACTATGAGCCTCACTTTGACCATG
  CTACGTCGCCCAGCAGCCCCCTGTACAGAATGAAGTCTGGGAACCGAGTGGCAACGTTTATGATCTATCT
  GAGCTCCGTAGAAGCTGGAGGAGCCACGGCTTTTATCTATGGCAACTTCAGTGTGCCTGTGGTTAAGAAT
  GCTGCACTGTTTTGGTGGAACCTGCACAGGAGCGGAGAGGGGGATGGTGACACACTTCATGCTGGCTGTC
  CTGTGCTGGTGGGAGACAAGTGGGTGGCCAACAAGTGGATCCACGAGTATGGACAGGAATTCCGAAGACC
  CTGCAGCACCAACCCTGAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206351 representing BC082538
  Red=Cloning site Green=Tags(s)
  
  MRLQDVYMLNVKGLARGVFQRVTGSSITDLYSPRQLFSLTADDCFQVGKVAYDTGDYYHAIPWLEEAVSL
  FRRAHGEWKTEDEASLEDALDYLAFACFQVGNVSCALSLSREFLVYSPDNKRMARNVLKYERLLAENGHQ
  MAAETAIQRPNVPHLQTRDTYEGLCQTLGSQPTHYQIPSLYCSYETNSSPYLLLQPARKEVVHLRPLIAL
  YHDFVSDEEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDTVDPMLVTLDHRIAALTGLDIQP
  PYAEYLQVVNYGIGGHYEPHFDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYGNFSVPVVKN
  AALFWWNLHRSGEGDGDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSTNPED
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC082538
• ORF Size: 1214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC082538, AAH82538
• RefSeq Size: 2426
• RefSeq ORF: 1214
• Locus ID: 320452