Bhmt (NM_016668) Mouse Tagged ORF Clone

CAT#: MG206402

Bhmt (GFP-tagged) - Mouse betaine-homocysteine methyltransferase (Bhmt)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bhmt
• Synonyms: MGC46866; MGC117976
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206402 representing NM_016668
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCAGTTGCTGGCAAGAAGGCCAAGAAGGGGATCTTAGAACGCTTAAATGCCGGAGAAGTTGTGA
  TTGGAGATGGAGGATTTGTCTTTGCACTGGAAAAGAGGGGCTATGTAAAGGCTGGACCCTGGACCCCAGA
  AGCTGCCGTGGAGCATCCTGAGGCAGTTCGTCAGCTTCATCGGGAGTTCCTCAGAGCTGGATCGAACGTC
  ATGCAGACCTTCACTTTCTATGCAAGTGAAGACAAGCTGGAAAACAGAGGAAACTATGTGGCAGAGAAGA
  TTTCTGGGCAGAAAGTCAACGAAGCTGCTTGTGACATTGCACGGCAAGTGGCTGATGAAGGAGACGCTTT
  GGTTGCAGGAGGCGTGAGCCAGACGCCTTCATACCTTAGCTGCAAGAGTGAGGTAGAAGTGAAAAAGATA
  TTTCGCCAACAGCTAGAGGTGTTCATGAAGAAGAACGTGGACTTCCTCATTGCAGAGTATTTTGAACATG
  TTGAAGAAGCCGTGTGGGCAGTGGAAGCCTTAAAAGCATCTGGTAAGCCCGTAGCAGCTACCATGTGCAT
  TGGGCCCGAGGGAGATCTGCATGGCGTGCCCCCTGGAGAGTGTGCCGTGCGTCTGGTGAAAGCAGGCGCC
  TCCATTGTCGGCGTGAACTGCCACTTCGACCCCAGCGTCAGCTTACAGACTGTGAAGCTCATGAAGGAGG
  GTTTGGAGGCTGCGCGGTTGAAAGCTTACCTGATGAGCCAGCCCCTGGCCTACCATACCCCTGACTGTGG
  CAAACAGGGATTTATTGATCTCCCAGAATTCCCCTTTGGATTGGAACCCCGAGTTGCCACTAGATGGGAT
  ATTCAAAAATATGCCAGAGAGGCCTACAACCTGGGGGTTAGGTACATTGGCGGCTGCTGCGGATTTGAGC
  CCTACCACATCAGGGCGATTGCAGAGGAGTTGGCCCCAGAAAGGGGATTTTTGCCACCGGCTTCAGAAAA
  ACATGGCAGCTGGGGAAGTGGTTTGGACATGCACACCAAACCCTGGATCAGAGCAAGGGCCAGAAAGGAA
  TACTGGCAGAATCTGCGAATAGCTTCCGGCAGGCCGTACAACCCTTCCATGTCCCGGCCAGATGCTTGGG
  GCGTGACTAAGGGAGCAGCCGAGCTGATGCAGCAGAAGGAGGCCACTACTGAGCAGCAGCTGAGAGAGCT
  CTTTGAAAAACAAAAATTCAAGTCTGCACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206402 representing NM_016668
  Red=Cloning site Green=Tags(s)
  
  MAPVAGKKAKKGILERLNAGEVVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNV
  MQTFTFYASEDKLENRGNYVAEKISGQKVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSEVEVKKI
  FRQQLEVFMKKNVDFLIAEYFEHVEEAVWAVEALKASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGA
  SIVGVNCHFDPSVSLQTVKLMKEGLEAARLKAYLMSQPLAYHTPDCGKQGFIDLPEFPFGLEPRVATRWD
  IQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARKE
  YWQNLRIASGRPYNPSMSRPDAWGVTKGAAELMQQKEATTEQQLRELFEKQKFKSAQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016668
• ORF Size: 1221 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016668.3, NP_057877.1
• RefSeq Size: 2081
• RefSeq ORF: 1224
• Locus ID: 12116
• Gene Summary: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. [UniProtKB/Swiss-Prot Function]