Spred2 (BC066013) Mouse Tagged ORF Clone

CAT#: MG206449

Spred2 (GFP-tagged) - Mouse sprouty-related, EVH1 domain containing 2 (cDNA clone MGC:90041 IMAGE:5706237)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spred2
• Synonyms: C79158
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206449 representing BC066013
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAAGAAACACACCCGGACGATGACAGCTATATTGTGCGTGTCAAGGCTGTGGTTATGACCAGAG
  ATGACTCCAGCGGGGGATGGTTCCCACAGGAAGGAGGCGGGATCAGTCGCGTCGGCGTGTGTAAGGTCAT
  GCACCCTGAAGGCAACGGACGAAGCGGCTTTCTCATCCATGGCGAGCGACAGAAAGACAAACTGGTGGTA
  TTGGAATGCTATGTCAGAAAGGACTTGGTCTACACCAAAGCCAATCCGACGTTTCATCATTGGAAGGTTG
  ATAACAGGAAGTTTGGACTTACTTTCCAAAGTCCTGCAGATGCACGAGCCTTTGACAGGGGCGTGAGAAA
  AGCCATTGAAGACCTTATAGAAGGTTCAACGACCTCCTCTTCCACTCTCCATAACGAAGCTGAGCTCGGA
  GACGATGACGTTTTCACGACAGCTACGGACAGTTCTTCTAATTCCTCGCAGAAGAGGGAGCCGACTACGA
  GGACAATCTCCTCCCCCACGTCCTGTGAGCACCGGAAGATTTATACCCTTGACCCATACCCCATGGACCA
  TTACCACCCTGACCAGCGGTTGCCGCGGTCCTACCCCCAGGTCACCTTCCCAGAAGATGATGAAGAAATT
  GTACGCATCAACCCCCGAGAGAAGATCTGGATGACCGGTTATGAAGACTACCGGCACGCGCCGGTTCGCG
  GCAAATACTTAGACACCACAGAAGACGCGGACTCCTACGTGCGCTTCGCCAAGGGCGAAGTCCCCAAACA
  CGAATATACCTATCCCTATGTTGATTCTTCGGACTTCGGCTTCGGGGAGGATCCCAAAGGTAGTGTGATC
  AAGACACAGCCGCCCAGGGCCAAGTCCCGTCGGCGGAAGGAGAACGGCGAACGGTCGCGGTGTGTGTACT
  GCAGGGATATGTTTAATCACGAAGAGAACCGAAGGGGCCACTGCCAAGACGCGCCCGACGCCGTGAGAAC
  TTGCATTCGCCGGGTGAGCTGTATGTGGTGCGCGGACAGCATGCTGTACCACTGTATGTCCGACCCCGAG
  GGAGACTACACTGACCCTTGTTCGTGTGACACAAGCGATGAGAAGTTTTGCCTCCGGTGGATGGCTCTAA
  TTGCCTTGTCTTTCCTGGCCCCTTGTATGTGCTGTTACCTGCCCCTCCGGGCCTGCCACCGCTGTGGAGT
  GATGTGCAGGTGCTGTGGTGGGAAGCACAAAGCCGCCGCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206449 representing BC066013
  Red=Cloning site Green=Tags(s)
  
  MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVV
  LECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELG
  DDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEI
  VRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVI
  KTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPE
  GDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC066013
• ORF Size: 1232 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC066013, AAH66013
• RefSeq Size: 2940
• RefSeq ORF: 1232
• Locus ID: 114716