Ass1 (BC002074) Mouse Tagged ORF Clone

CAT#: MG206472

Ass1 (GFP-tagged) - Mouse argininosuccinate synthetase 1 (cDNA clone MGC:6218 IMAGE:3491910)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ass1
• Synonyms: ASS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206472 representing BC002074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGCAAGGGCTCTGTGGTTCTGGCCTACAGTGGTGGCCTGGACACCTCCTGCATCCTCGTGTGGC
  TGAAGGAACAAGGCTATGATGTCATCGCCTACCTGGCCAACATTGGCCAGAAGGAAGACTTTGAGGAAGC
  CAGGAAGAAGGCGCTGAAGCTTGGGGCCAAAAAGGTGTTCATTGAGGATGTGAGCAAGGAATTTGTGGAA
  GAGTTCATCTGGCCTGCTGTCCAGTCCAGTGCACTCTACGAGGACCGCTATCTCCTGGGCACCTCTCTCG
  CCAGGCCTTGCATAGCTCGCAGACAGGTGGAGATTGCCCAGCGTGAAGGGGCCAAGTATGTGTCTCACGG
  CGCCACGGGAAAGGGGAATGACCAGGTCCGCTTTGAGCTCACCTGCTACTCACTGGCACCCCAGATTAAG
  GTCATCGCTCCCTGGAGGATGCCTGAGTTTTACAACCGGTTCAAGGGCCGAAATGATCTGATGGAGTATG
  CAAAGCAACACGGAATCCCCATCCCTGTCACCCCCAAGAGCCCCTGGAGTATGGATGAAAACCTCATGCA
  CATCAGCTATGAGGCTGGGATCCTGGAAAACCCCAAGAATCAAGCACCTCCGGGTCTCTACACAAAAACT
  CAGGACCCTGCCAAAGCACCCAACAGCCCAGATGTCCTTGAGATAGAATTCAAAAAAGGGGTCCCTGTGA
  AGGTGACCAACATCAAAGATGGCACAACCCGCACCACATCCCTGGAACTCTTCATGTACCTGAACGAAGT
  TGCGGGCAAGCACGGAGTGGGTCGCATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT
  ATCTACGAGACCCCAGCAGGGACCATCCTTTACCACGCTCATTTAGACATAGAGGCCTTCACGATGGATC
  GGGAAGTACGCAAAATCAAGCAGGGCCTGGGCCTCAAATTCGCAGAGCTCGTATACACAGGTTTCTGGCA
  CAGCCCTGAATGTGAATTTGTTCGCCACTGTATCCAGAAGTCCCAGGAGCGGGTAGAAGGGAAGGTGCAG
  GTGTCTGTCTTCAAGGGCCAAGTGTACATCCTCGGTCGGGAGTCTCCACTTTCACTCTACAATGAAGAGC
  TGGTGAGCATGAACGTACAGGGCGACTATGAGCCCATCGACGCCACTGGCTTCATCAATATCAACTCGCT
  CAGGCTGAAGGAGTACCATCGCCTTCAGAGCAAGGTCACTGCCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206472 representing BC002074
  Red=Cloning site Green=Tags(s)
  
  MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSKEFVE
  EFIWPAVQSSALYEDRYLLGTSLARPCIARRQVEIAQREGAKYVSHGATGKGNDQVRFELTCYSLAPQIK
  VIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKSPWSMDENLMHISYEAGILENPKNQAPPGLYTKT
  QDPAKAPNSPDVLEIEFKKGVPVKVTNIKDGTTRTTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRG
  IYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIQKSQERVEGKVQ
  VSVFKGQVYILGRESPLSLYNEELVSMNVQGDYEPIDATGFININSLRLKEYHRLQSKVTAK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC002074
• ORF Size: 1238 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC002074, AAH02074
• RefSeq Size: 1645
• RefSeq ORF: 1238
• Locus ID: 11898