Acadm (NM_007382) Mouse Tagged ORF Clone

CAT#: MG206704

Acadm (GFP-tagged) - Mouse acyl-Coenzyme A dehydrogenase, medium chain (Acadm)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acadm
• Synonyms: AU018656; MCAD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206704 representing NM_007382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGCGTTCCGCAGAGGCTGCAGGGTCCTGAGAAGTGTTTCTCATTTTGAGTGTCGAACACAAC
  ACTCGAAAGCGGCTCACAAGCAGGAGCCCGGATTAGGGTTTAGTTTTGAGTTGACGGAACAGCAGAAAGA
  GTTTCAAGCAACTGCCCGCAAGTTTGCCAGAGAGGAGATTATCCCCGTCGCCCCGGAATATGACAAAAGC
  GGGGAGTACCCGTTCCCTCTCATCAAAAGAGCCTGGGAACTCGGCTTGATCAACGCGCACATTCCGGAAA
  GTTGCGGTGGTCTTGGCCTGGGAACGTTCGATGCTTGTTTAATTACCGAAGAGTTGGCGTATGGGTGTAC
  AGGGGTGCAAACTGCTATTGAAGCAAATTCTTTGGGGCAAATGCCTGTGATTCTTGCTGGAAATGATCAA
  CAAAAAAAGAAGTATTTGGGGAGGATGACGGAGCAGCCAATGATGTGTGCTTACTGTGTGACAGAGCCCT
  CCGCAGGCTCTGATGTGGCGGCCATTAAGACCAAAGCAGAGAAGAAGGGTGACGAGTATGTTATCAACGG
  CCAGAAGATGTGGATAACCAACGGGGGAAAGGCCAACTGGTATTTCTTGTTGGCGCGTTCTAACCCAGAT
  CCTAAAGTACCCGCTAGTAAAGCCTTTACTGGATTCATTGTGGAAGCCGACACCCCGGGAATACATATTG
  GAAAAAAGGAACTAAACATGGGCCAGCGATGCTCTGACACCAGAGGAATTGCTTTCGAAGACGTCAGAGT
  GCCTAAGGAAAATGTGTTAATCGGTGAAGGAGCAGGTTTCAAGATCGCAATGGGTGCTTTTGATAGAACC
  AGACCTACAGTCGCAGCTGGCGCTGTCGGGCTAGCCCAGAGAGCTCTGGACGAAGCCACGAAGTATGCCC
  TGGATAGGAAGACATTTGGAAAGCTGCTAGTGGAGCACCAAGGAGTTTCATTTCTGCTCGCAGAAATGGC
  GATGAAGGTTGAACTCGCTAGGCTCAGTTACCAGAGAGCAGCCTGGGAGGTTGACTCCGGTCGCCGGAAC
  ACTTACTATGCCTCGATTGCAAAGGCCTGTGCTGGAGACATTGCCAATCAGCTAGCCACTGACGCCGTGC
  AGATTTTCGGAGGCTATGGATTCAACACAGAGTACCCTGTGGAGAAGCTGATGAGGGACGCCAAGATCTA
  TCAGATTTATGAAGGTACTGCACAAATTCAGAGGCTGATCATAGCTCGTGAGCACATTGAAAAGTATAAA
  AAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206704 representing NM_007382
  Red=Cloning site Green=Tags(s)
  
  MAAAFRRGCRVLRSVSHFECRTQHSKAAHKQEPGLGFSFELTEQQKEFQATARKFAREEIIPVAPEYDKS
  GEYPFPLIKRAWELGLINAHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGQMPVILAGNDQ
  QKKKYLGRMTEQPMMCAYCVTEPSAGSDVAAIKTKAEKKGDEYVINGQKMWITNGGKANWYFLLARSNPD
  PKVPASKAFTGFIVEADTPGIHIGKKELNMGQRCSDTRGIAFEDVRVPKENVLIGEGAGFKIAMGAFDRT
  RPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELARLSYQRAAWEVDSGRRN
  TYYASIAKACAGDIANQLATDAVQIFGGYGFNTEYPVEKLMRDAKIYQIYEGTAQIQRLIIAREHIEKYK
  N
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007382
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007382.1, NP_031408.1
• RefSeq Size: 1846
• RefSeq ORF: 1266
• Locus ID: 11364
• Gene Summary: This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C6- and C12-acylCoA. In mice, deficiency of this gene can cause neonatal mortality as well as fasting and cold intolerance. This gene has multiple, intronless pseudogenes. [provided by RefSeq, Nov 2012]