Ambn (BC087927) Mouse Tagged ORF Clone

CAT#: MG206721

Ambn (GFP-tagged) - Mouse ameloblastin (cDNA clone MGC:107285 IMAGE:30504437)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ambn
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206721 representing BC087927
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCATCTAAGATTCCACTTTTCAAAATGAAGGGCCTGATCCTGTTCCTGTCCCTAGTGAAAATGA
  GCCTCGCCGTGCCGGCATTTCCTCAACAACCTGGGGCTCAAGGCATGGCACCTCCTGGCATGGCTAGTTT
  GAGCCTTGAGACAATGAGACAGTTGGGAAGCTTGCAGGGACTCAACGCACTTTCTCAGTATTCTAGACTT
  GGCTTTGGAAAAGCACTTAATAGTTTATGGTTGCACGGACTTCTCCCACCGCATAACTCTTTCCCATGGA
  TAGGACCAAGGGAACATGAAACCCAGCAGTATGAATATTCTTTGCCTGTGCATCCCCCACCTCTCCCATC
  ACAGCCATCCTTGCAGCCTCACCAGCCAGGACTGAAACCCTTCCTCCAGCCCACTGCTGCAACCGGTGTC
  CAGGTCACACCCCAGAAGCCAGGGCCTCAGCCTCCAATGCACCCTGGACAGCTGCCCTTGCAGGAAGGAG
  AGCTGATAGCACCAGATGAGCCGCAGGTGGCACCATCCGAAAACCCACCAACACCTGAGGTACCAATAAT
  GGATTTTGCTGATCCACAATTTCCAACCGTGTTCCAGATCGCCCGTTCAATATCTCGGGGACCAATGGCA
  CACAACAAAGCATCCGCTTTTTACCCAGGAATGTTTTACATGTCTTATGGAGCAAACCAATTGAATGCTC
  CTGCCAGAATTGGCTTCATGAGTTCAGAAGAAATGCCTGGAGAAAGAGGAAGTCCCATGGCCTATGGAAC
  TCTGTTCCCAAGATTTGGAGGCTTCAGGCAAACCCTTAGGAGACTGAATCAGAATTCACCCAAGGGAGGA
  GACTTTACTGTGGAAGTAGATTCCCCAGTATCTGTTACCAAAGGCCCTGAAAAAGGAGAAGGTCCAGAAG
  GCTCTCCACTGCAAGAGGCCAACCCAGGCAAACGGGAAAACCCCGCTCTCCTTTCACAAATGGCACCTGG
  GGCCCATGCAGGACTTCTTGCTTTCCCCAATGACCACATCCCCAGTATGGCAAGGGGTCCTGCAGGGCAA
  AGACTCCTTGGAGTCACCCCTGCAGCTGCAGACCCACTGATCACCCCTGAATTAGCAGAAGTTTATGAAA
  CCTATGGTGCTGATGTTACCACACCCCTGGGTGATGGAGAAGCAACCATGGATATCACCATGTCCCCAGA
  CACTCAGCAGCCACTGCTACCTGGAAACAAAGTGCACCAGCCCCAGGTGCACAACGCATGGCGTTTCCAA
  GAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206721 representing BC087927
  Red=Cloning site Green=Tags(s)
  
  MSASKIPLFKMKGLILFLSLVKMSLAVPAFPQQPGAQGMAPPGMASLSLETMRQLGSLQGLNALSQYSRL
  GFGKALNSLWLHGLLPPHNSFPWIGPREHETQQYEYSLPVHPPPLPSQPSLQPHQPGLKPFLQPTAATGV
  QVTPQKPGPQPPMHPGQLPLQEGELIAPDEPQVAPSENPPTPEVPIMDFADPQFPTVFQIARSISRGPMA
  HNKASAFYPGMFYMSYGANQLNAPARIGFMSSEEMPGERGSPMAYGTLFPRFGGFRQTLRRLNQNSPKGG
  DFTVEVDSPVSVTKGPEKGEGPEGSPLQEANPGKRENPALLSQMAPGAHAGLLAFPNDHIPSMARGPAGQ
  RLLGVTPAAADPLITPELAEVYETYGADVTTPLGDGEATMDITMSPDTQQPLLPGNKVHQPQVHNAWRFQ
  EP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC087927
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC087927.1
• RefSeq Size: 1602
• RefSeq ORF: 1268
• Locus ID: 11698
• Gene Summary: This gene encodes an extracellular matrix glycoprotein that is involved in the formation of dental enamel. Mice lacking the encoded protein fail to undergo normal ameloblast differentiation and develop enamel. Mice overproducing the product of this gene develop thinner and more porous enamel, with disrupted rod patterns and abnormal crystallites. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]