Pnpla2 (BC019188) Mouse Tagged ORF Clone

CAT#: MG206855

Pnpla2 (GFP-tagged) - Mouse patatin-like phospholipase domain containing 2 (cDNA clone MGC:29206 IMAGE:5027544)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pnpla2
• Synonyms: TTS-2.2, Atgl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206855 representing BC019188
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCATGCCTGCCAAGGTGAAGCAGGTGCCAACATTATTGAGGTGTCCAAGGAGGCCCGGAAGCGGT
  TCCTGGGTCCTCTGCATCCCTCCTTCAACCTGGTGAAGACCATCCGTGGCTGTCTACTAAAGACCCTGCC
  TGCTGATTGCCATGAGCGCGCCAATGGACGCCTGGGCATCTCCCTGACTCGTGTTTCAGACGGAGAGAAC
  GTCATCATATCCCACTTTAGCTCCAAGGATGAGCTCATCCAGGCCAATGTCTGCAGCACATTTATCCCGG
  TGTACTGTGGCCTCATTCCTCCTACCCTCCAAGGGGTGCGCTATGTGGATGGCGGCATTTCAGACAACTT
  GCCACTTTATGAGCTGAAGAATACCATCACAGTGTCCCCATTCTCAGGCGAGAGTGACATCTGCCCTCAG
  GACAGCTCCACCAACATCCACGAGCTTCGCGTCACCAACACCAGCATCCAGTTCAACCTTCGCAATCTCT
  ACCGCCTCTCGAAGGCTCTCTTCCCGCCAGAGCCCATGGTCCTCCGAGAGATGTGCAAACAGGGCTACAG
  AGATGGACTTCGATTCCTTAGGAGGAATGGCCTACTGAACCAACCCAACCCTTTGCTGGCACTGCCCCCA
  GTTGTCCCCCAGGAAGAGGATGCAGAGGAAGCTGCTGTGGTGGAGGAGAGGGCTGGAGAGGAGGATCAAT
  TGCAGCCTTATAGAAAAGATCGAATTCTAGAGCACCTGCCTGCCAGACTCAATGAGGCCCTGCTGGAGGC
  CTGTGTGGAACCAAAGGACCTGATGACCACCCTTTCCAACATGCTACCAGTGCGCCTGGCAACGGCCATG
  ATGGTGCCCTATACTCTGCCGCTGGAGAGTGCAGTGTCCTTCACCATCCGCTTGTTGGAGTGGCTGCCTG
  ATGTCCCTGAAGATATCCGGTGGATGAAAGAGCAGACGGGTAGCATCTGCCAGTATCTGGTGATGAGGGC
  CAAGAGGAAATTGGGTGACCATCTGCCTTCCAGACTGTCTGAGCAGGTGGAACTGCGACGTGCCCAGTCT
  CTGCCCTCTGTGCCACTGTCTTGCGCCACCTACAGTGAGGCCCTACCCAACTGGGTACGAAACAACCTCT
  CACTGGGGGACGCGCTGGCCAAGTGGGAAGAATGCCAGCGTCAGCTACTGCTGGGTCTCTTCTGCACCAA
  TGTGGCCTTCCCGCCGGATGCCTTGCGCATGCGCGCACCTGCCAGCCCCACTGCCGCAGATCCTGCCACC
  CCACAGGATCCACCTGGCCTCCCGCCTTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206855 representing BC019188
  Red=Cloning site Green=Tags(s)
  
  MSHACQGEAGANIIEVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVSDGEN
  VIISHFSSKDELIQANVCSTFIPVYCGLIPPTLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQ
  DSSTNIHELRVTNTSIQFNLRNLYRLSKALFPPEPMVLREMCKQGYRDGLRFLRRNGLLNQPNPLLALPP
  VVPQEEDAEEAAVVEERAGEEDQLQPYRKDRILEHLPARLNEALLEACVEPKDLMTTLSNMLPVRLATAM
  MVPYTLPLESAVSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGDHLPSRLSEQVELRRAQS
  LPSVPLSCATYSEALPNWVRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPDALRMRAPASPTAADPAT
  PQDPPGLPPC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC019188
• ORF Size: 1290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC019188.1
• RefSeq Size: 2064
• RefSeq ORF: 1292
• Locus ID: 66853