Ap2m1 (NM_009679) Mouse Tagged ORF Clone

CAT#: MG206935

Ap2m1 (GFP-tagged) - Mouse adaptor protein complex AP-2, mu1 (Ap2m1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ap2m1
• Synonyms: MGC102136
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206935 representing NM_009679
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTTATCTCCCGGGTCTACAGAGATGACA
  TCGGGAGGAATGCTGTGGATGCCTTTCGGGTCAATGTCATTCATGCACGGCAGCAGGTGCGCAGCCCTGT
  CACAAACATCGCTCGCACCAGCTTCTTCCATGTTAAGCGGTCCAACATCTGGCTGGCCGCAGTCACCAAG
  CAGAATGTCAATGCTGCCATGGTCTTCGAATTCCTCTACAAGATGTGTGATGTAATGGCTGCTTACTTTG
  GCAAAATCAGCGAGGAGAACATCAAGAACAATTTTGTGCTCATATACGAGCTGCTGGATGAGATTCTGGA
  CTTTGGCTACCCACAGAACTCAGAGACAGGTGCACTGAAAACCTTCATCACCCAGCAGGGTATCAAGAGT
  CAGCATCAGACGAAGGAAGAACAGTCCCAGATCACCAGCCAGGTGACCGGGCAGATTGGCTGGCGGCGAG
  AAGGCATCAAGTATCGCCGGAATGAACTCTTCCTAGATGTTCTGGAGAGTGTGAACCTGCTTATGTCCCC
  ACAGGGGCAGGTGCTGAGTGCCCATGTGTCAGGCCGGGTGGTGATGAAGAGTTACCTGAGTGGCATGCCT
  GAGTGCAAGTTTGGAATGAATGACAAGATTGTCATAGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAA
  GCAAGAGTGGTAAGCAGTCGATCGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTT
  TGACTCTGAGCGGAGCATCAGCTTCATCCCTCCCGACGGAGAGTTTGAACTCATGAGATACCGTACTACC
  AAGGACATCATCCTTCCTTTCCGGGTGATCCCATTGGTGCGGGAGGTGGGGCGCACCAAACTGGAGGTCA
  AGGTGGTCATCAAGTCCAACTTCAAGCCCTCACTTCTGGCCCAGAAGATTGAGGTGAGGATCCCGACTCC
  ATTGAACACAAGCGGGGTACAGGTGATCTGCATGAAGGGGAAGGCCAAGTACAAGGCCAGCGAGAACGCC
  ATTGTATGGAAGATCAAGCGCATGGCAGGCATGAAGGAATCCCAGATCAGCGCAGAGATTGAGCTCCTGC
  CCACCAACGATAAGAAGAAATGGGCTCGGCCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCGCCTTC
  TGGCCTCAAGGTGCGCTACTTGAAGGTATTCGAACCGAAGCTGAACTACAGCGACCATGATGTCATCAAA
  TGGGTGCGATACATTGGCCGCAGTGGCATTTATGAAACCCGCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206935 representing NM_009679
  Red=Cloning site Green=Tags(s)
  
  MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTK
  QNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKS
  QHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMP
  ECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTT
  KDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENA
  IVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIK
  WVRYIGRSGIYETRC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009679
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009679.2, NP_033809.1
• RefSeq Size: 2057
• RefSeq ORF: 1308
• Locus ID: 11773
• Gene Summary: This gene encodes the mu subunit of the clathrin adaptor protein complex AP-2. It mediates sorting of cargo proteins harboring Y-X-X-Phi motifs into clathrin-coated vesicles. Alternate splicing of this gene results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2, 8 and 19. [provided by RefSeq, Dec 2014]