Ap2m1 (NM_009679) Mouse Tagged ORF Clone

CAT#: MR206935

Ap2m1 (Myc-DDK-tagged) - Mouse adaptor protein complex AP-2, mu1 (Ap2m1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ap2m1
• Synonyms: MGC102136
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206935 representing NM_009679
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTTATCTCCCGGGTCTACAGAGATGACA
  TCGGGAGGAATGCTGTGGATGCCTTTCGGGTCAATGTCATTCATGCACGGCAGCAGGTGCGCAGCCCTGT
  CACAAACATCGCTCGCACCAGCTTCTTCCATGTTAAGCGGTCCAACATCTGGCTGGCCGCAGTCACCAAG
  CAGAATGTCAATGCTGCCATGGTCTTCGAATTCCTCTACAAGATGTGTGATGTAATGGCTGCTTACTTTG
  GCAAAATCAGCGAGGAGAACATCAAGAACAATTTTGTGCTCATATACGAGCTGCTGGATGAGATTCTGGA
  CTTTGGCTACCCACAGAACTCAGAGACAGGTGCACTGAAAACCTTCATCACCCAGCAGGGTATCAAGAGT
  CAGCATCAGACGAAGGAAGAACAGTCCCAGATCACCAGCCAGGTGACCGGGCAGATTGGCTGGCGGCGAG
  AAGGCATCAAGTATCGCCGGAATGAACTCTTCCTAGATGTTCTGGAGAGTGTGAACCTGCTTATGTCCCC
  ACAGGGGCAGGTGCTGAGTGCCCATGTGTCAGGCCGGGTGGTGATGAAGAGTTACCTGAGTGGCATGCCT
  GAGTGCAAGTTTGGAATGAATGACAAGATTGTCATAGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAA
  GCAAGAGTGGTAAGCAGTCGATCGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTT
  TGACTCTGAGCGGAGCATCAGCTTCATCCCTCCCGACGGAGAGTTTGAACTCATGAGATACCGTACTACC
  AAGGACATCATCCTTCCTTTCCGGGTGATCCCATTGGTGCGGGAGGTGGGGCGCACCAAACTGGAGGTCA
  AGGTGGTCATCAAGTCCAACTTCAAGCCCTCACTTCTGGCCCAGAAGATTGAGGTGAGGATCCCGACTCC
  ATTGAACACAAGCGGGGTACAGGTGATCTGCATGAAGGGGAAGGCCAAGTACAAGGCCAGCGAGAACGCC
  ATTGTATGGAAGATCAAGCGCATGGCAGGCATGAAGGAATCCCAGATCAGCGCAGAGATTGAGCTCCTGC
  CCACCAACGATAAGAAGAAATGGGCTCGGCCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCGCCTTC
  TGGCCTCAAGGTGCGCTACTTGAAGGTATTCGAACCGAAGCTGAACTACAGCGACCATGATGTCATCAAA
  TGGGTGCGATACATTGGCCGCAGTGGCATTTATGAAACCCGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206935 representing NM_009679
  Red=Cloning site Green=Tags(s)
  
  MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTK
  QNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKS
  QHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMP
  ECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTT
  KDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENA
  IVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIK
  WVRYIGRSGIYETRC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009679
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009679.1, NM_009679.2, NM_009679.3, NP_033809.1
• RefSeq Size: 2057 bp
• RefSeq ORF: 1308 bp
• Locus ID: 11773
• MW: 50.1 kDa
• Gene Summary: This gene encodes the mu subunit of the clathrin adaptor protein complex AP-2. It mediates sorting of cargo proteins harboring Y-X-X-Phi motifs into clathrin-coated vesicles. Alternate splicing of this gene results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2, 8 and 19. [provided by RefSeq, Dec 2014]