Gcm1 (BC066866) Mouse Tagged ORF Clone

CAT#: MG206959

Gcm1 (GFP-tagged) - Mouse glial cells missing homolog 1 (Drosophila) (cDNA clone MGC:76737 IMAGE:30432193)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gcm1
• Synonyms: Gcm-rs2, glide, GCMa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206959 representing BC066866
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGGACGACTTTGATCCTGAAGACAAAGAGATACTGAGCTGGGACATTAACGATGTGAAACTGC
  CTCAGAACGTGAAAACGACTGACTGGTTCCAGGAGTGGCCGGACTCCTACGTGAAACACATCTACAGCTC
  GGACGACAGGAACGCACAGCGCCACCTGAGCAGCTGGGCCATGCGCAACACCAACAACCACAACTCCCGG
  ATCCTCAAGAAGTCATGCCTGGGGGTAGTGGTGTGCAGCAGGGACTGCTCCACAGAGGAAGGCCGCAAGA
  TTTACCTGAGACCCGCCATCTGTGACAAAGCCAGACAGAAGCAGCAGAGGAAAAGCTGTCCCAACTGCAA
  TGGACCCCTGAAGCTTATTCCCTGCCGAGGCCACGGCGGCTTCCCGGTCACCAACTTCTGGAGGCACGAC
  GGACGCTTCATCTTTTTCCAGTCCAAAGGCGAGCATGATCATCCCAGGCCGGAGACCAAGCTGGAAGCAG
  AGGCAAGAAGAGCCATGAAGAAAGTGCACATGGCATCTGCCTCCAACTCCTTACGGATGAAGGGGAGACC
  AGCAGCGAAGGCGCTTCCTGCTGAAATCCCGAGTCAGGGAAGTTTACCTTTAACTTGGTCATTCCAGGAA
  GGCGTCCAACTGCCCGGCACTTACAGCACACCTTTAATAGCTAACGCCCCCCAGCAGAACTCCCTGAATG
  ATTGCTTATCCTTCCCCAAGAGTTATGATTTGGGGGGAAGCACTGAGCTGGAAGATCCAACTTCCACCTT
  AGATTCCATGAAGTTCTATGAGAGATGCAAATTCTCCAGCAGTAGGATCTACGGCAGTGAAGAGCAGTTT
  CAGCCTCCTGTCCCTGGGACGTATGGAGACTACGAAGACCTGCAAACTTGGAATAAAAATGTTGCCTTAG
  GGAGAAATCCCTCCGATGACATCTACTATCCAGCCTATCCTCTGCCTGTGGCCAGCTGGCCCTACGACTA
  CTTTCCCTCCCAGAACTCTTTGGAGCACTTACCCCAGCAAGTTCCATCAGAACCCCCTGCAGCTCAACCA
  GGCTGTCATCCCTTGTGGTCCAATCCGGGAGGTGAGCCTTACGAAGAGAAAGTATCTGTGGATCTGAGCA
  GCTATGTGCCCTCCCTCACGTACCACCCACCTCAGCAGGATCCCTTCCTGCTCACCTATGGCTCTCCTAC
  TCAGCAGCAACATGCACTGCCCGGCAAGAGCAACAGGTGGGATTTTGACGAAGAGATGGCATGCATGGGC
  TTAGATCACTTCAACAATGAGATGCTCCTAAACTTCTGTTCTTTAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206959 representing BC066866
  Red=Cloning site Green=Tags(s)
  
  MELDDFDPEDKEILSWDINDVKLPQNVKTTDWFQEWPDSYVKHIYSSDDRNAQRHLSSWAMRNTNNHNSR
  ILKKSCLGVVVCSRDCSTEEGRKIYLRPAICDKARQKQQRKSCPNCNGPLKLIPCRGHGGFPVTNFWRHD
  GRFIFFQSKGEHDHPRPETKLEAEARRAMKKVHMASASNSLRMKGRPAAKALPAEIPSQGSLPLTWSFQE
  GVQLPGTYSTPLIANAPQQNSLNDCLSFPKSYDLGGSTELEDPTSTLDSMKFYERCKFSSSRIYGSEEQF
  QPPVPGTYGDYEDLQTWNKNVALGRNPSDDIYYPAYPLPVASWPYDYFPSQNSLEHLPQQVPSEPPAAQP
  GCHPLWSNPGGEPYEEKVSVDLSSYVPSLTYHPPQQDPFLLTYGSPTQQQHALPGKSNRWDFDEEMACMG
  LDHFNNEMLLNFCSLR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC066866
• ORF Size: 1310 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC066866, AAH66866
• RefSeq Size: 2048
• RefSeq ORF: 1310
• Locus ID: 14531