Becn1 (BC005770) Mouse Tagged ORF Clone

CAT#: MG207162

Becn1 (GFP-tagged) - Mouse beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) (cDNA clone MGC:6843 IMAGE:2650059)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Becn1
• Synonyms: 4921513J16Rik, 5430417M23Rik, MGC6843
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207162 representing BC005770
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGTCTAAGGCGTCCAGCAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGCTGTAGCCAGCCTC
  TGAAACTGGACACGAGCTTCAAGATCCTGGACCGGGTCACCATCCAGGAACTCACAGCTCCATTACTTAC
  CACAGCCCAGGCGAAACCAGGAGAGACCCAGGAGGAAGAGGCTAACTCAGGAGAGGAGCCATTTATTGAA
  ACTCGCCAGGATGGTGTCTCTCGAAGATTCATCCCCCCAGCCAGGATGATGTCTACAGAAAGTGCTAATA
  GCTTCACTCTGATCGGGGAGGCATCTGATGGTGGCACCATGGAGAACCTCAGCCGGAGACTCAAGGTCAC
  TGGAGACCTGTTTGACATCATGTCGGGCCAGACCGATGTGGATCACCCGCTGTGTGAGGAATGCACAGAC
  ACTCTTTTAGACCAGCTGGACACTCAGCTCAATGTCACTGAGAATGAATGTCAGAACTACAAACGCTGTT
  TGGAGATCCTAGAGCAGATGAATGAGGATGACAGTGAGCAGCTGCAGAGGGAGCTGAAGGAGCTGGCCTT
  GGAGGAGGAGAGGCTGATCCAGGAGCTGGAAGATGTGGAAAAGAACCGCAAGGTGGTGGCAGAGAACCTG
  GAGAAGGTCCAGGCTGAGGCGGAGAGATTGGACCAGGAGGAAGCTCAGTACCAGCGGGAGTATAGTGAGT
  TTAAAAGGCAGCAGCTGGAGTTGGATGACGAACTCAAGAGTGTGGAGAACCAGGTGCGCTACGCCCAGAT
  CCAGCTGGACAAGCTCAAGAAAACCAATGTCTTCAATGCCACCTTCCACATCTGGCACAGCGGACAGTTT
  GGCACAATCAATAATTTCAGACTGGGTCGCTTGCCCAGTGTTCCTGTGGAGTGGAATGAAATCAATGCTG
  CCTGGGGCCAGACAGTGCTGCTGCTCCATGCTTTGGCCAATAAGATGGGTCTGAAGTTTCAGAGGTACCG
  ACTTGTTCCCTATGGAAATCATTCCTATCTGGAGTCTCTGACAGACAAATCTAAGGAGTTGCCGTTATAC
  TGTTCTGGGGGTTTGCGGTTTTTCTGGGACAACAAGTTTGACCATGCAATGGTAGCTTTTCTGGACTGTG
  TGCAGCAGTTCAAAGAAGAGGTGGAAAAAGGAGAGACTCGATTTTGTCTTCCGTACAGGATGGACGTGGA
  GAAAGGCAAGATTGAAGACACTGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAACTCGGAG
  GAGCAGTGGACAAAAGCGCTCAAGTTCATGCTGACCAATCTCAAGTGGGGTCTTGCCTGGGTGTCCTCAC
  AGTTCTATAACAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207162 representing BC005770
  Red=Cloning site Green=Tags(s)
  
  MEGSKASSSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEEANSGEEPFIE
  TRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTD
  TLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENL
  EKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQVRYAQIQLDKLKKTNVFNATFHIWHSGQF
  GTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLY
  CSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSE
  EQWTKALKFMLTNLKWGLAWVSSQFYNK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005770
• ORF Size: 1344 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC005770, AAH05770
• RefSeq Size: 2034
• RefSeq ORF: 1346
• Locus ID: 56208