Cs (NM_026444) Mouse Tagged ORF Clone

CAT#: MG207418

Cs (GFP-tagged) - Mouse citrate synthase (Cs)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cs
• Synonyms: 2610511A05Rik; 9030605P22Rik; Ahl4; BB234005; Cis
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207418 representing NM_026444
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTACTCACTGCAGCAACCCGGCTCTTGGGAGCCAAGAACTCATCCTGCCTCGTCCTTGCTGCCC
  GGCATGCCAGTGCTTCTTCCACGAATTTGAAAGATGTACTGAGCAATCTGATACCTAAGGAGCAGGCCAG
  AATTAAGACCTTCAAGCAGCAACATGGGAAGACAGTGGTGGGCCAGATCACTGTGGACATGATGTACGGT
  GGCATGAGAGGCATGAAGGGACTTGTGTATGAGACTTCGGTTCTTGATCCTGACGAGGGCATCCGTTTCC
  GAGGCTACAGTATCCCTGAGTGCCAGAAAATGCTGCCTAAGGCTAAGGGTGGAGAAGAACCCCTGCCTGA
  GGGCTTATTTTGGCTGCTGGTAACTGGACAGATGCCCACAGAGGAACAGGTGTCTTGGCTCTCACGAGAA
  TGGGCAAAAAGGGCAGCTCTGCCTTCTCATGTGGTCACCATGCTGGACAATTTTCCAACCAATCTGCACC
  CTATGTCTCAGCTCAGTGCAGCCATCACAGCCCTCAACAGTGAAAGCAACTTCGCCCGGGCATATGCAGA
  GGGAATGAACCGAGCTAAGTACTGGGAGCTCATCTATGAGGACTGCATGGACCTCATTGCCAAGCTGCCG
  TGCGTTGCAGCCAAGATCTACCGGAATCTGTACCGGGAGGGCAGCAGTATCGGAGCCATTGACTCTAGGC
  TAGACTGGTCACACAATTTTACCAACATGTTAGGCTACACCGACCCTCAGTTCACCGAGCTCATGCGTTT
  GTACCTCACCATCCATAGTGACCATGAGGGTGGTAATGTAAGTGCCCACACAAGCCATTTGGTGGGCAGC
  GCCCTTTCAGACCCTTACCTGTCCTTTGCAGCAGCCATGAATGGGCTGGCGGGGCCTCTACATGGACTAG
  CAAATCAGGAGGTGCTTGTCTGGCTGACACAGCTACAGAAGGAAGTTGGCAAAGACGTGTCAGATGAGAA
  GTTACGAGACTACATCTGGAACACACTCAATTCAGGACGGGTGGTCCCAGGATACGGTCATGCAGTACTG
  AGGAAGACTGACCCTCGCTATTCCTGTCAGCGAGAGTTTGCTCTGAAACATCTGCCTAAAGATCCCATGT
  TCAAGCTGGTGGCTCAGCTGTACAAGATTGTGCCCAATATCCTCTTAGAGCAAGGGAAGGCTAAGAACCC
  TTGGCCCAACGTAGACGCTCACAGTGGGGTGCTGCTCCAGTACTATGGCATGACGGAGATGAACTACTAC
  ACAGTCCTGTTTGGAGTGTCTCGGGCACTGGGTGTGCTAGCCCAGCTCATCTGGAGCAGAGCCCTAGGCT
  TCCCCCTGGAAAGGCCCAAGTCCATGAGCACGGATGGCCTGATGAAGTTTGTGGACTCTAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207418 representing NM_026444
  Red=Cloning site Green=Tags(s)
  
  MALLTAATRLLGAKNSSCLVLAARHASASSTNLKDVLSNLIPKEQARIKTFKQQHGKTVVGQITVDMMYG
  GMRGMKGLVYETSVLDPDEGIRFRGYSIPECQKMLPKAKGGEEPLPEGLFWLLVTGQMPTEEQVSWLSRE
  WAKRAALPSHVVTMLDNFPTNLHPMSQLSAAITALNSESNFARAYAEGMNRAKYWELIYEDCMDLIAKLP
  CVAAKIYRNLYREGSSIGAIDSRLDWSHNFTNMLGYTDPQFTELMRLYLTIHSDHEGGNVSAHTSHLVGS
  ALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVL
  RKTDPRYSCQREFALKHLPKDPMFKLVAQLYKIVPNILLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYY
  TVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTDGLMKFVDSK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026444
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_026444.3, NP_080720.1
• RefSeq Size: 2828
• RefSeq ORF: 1395
• Locus ID: 12974
• Gene Summary: The protein encoded by this gene is a central metabolic pathway enzyme, catalyzing the first step of the tricarboxylic acid cycle in which acetyl coenzyme A and oxaloacetate are converted to citrate and coenzyme A. This enzyme is found in nearly all cells capable of oxidative metabolism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2016]