Smad5 (BC050001) Mouse Tagged ORF Clone

CAT#: MG207430

Smad5 (GFP-tagged) - Mouse MAD homolog 5 (Drosophila) (cDNA clone MGC:58953 IMAGE:6490853)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Smad5
• Synonyms: Dwf-C, MusMLP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207430 representing BC050001
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCAATGGCCAGCTTGTTTTCTTTCACTAGTCCAGCCGTGAAGCGATTGTTGGGCTGGAAACAAG
  GTGACGAGGAAGAGAAATGGGCAGAAAAGGCAGTGGATGCTTTAGTGAAAAAGCTGAAGAAGAAGAAGGG
  TGCTATGGAGGAGCTGGAGAAAGCCTTGAGCAGCCCAGGACAGCCAAGCAAGTGTGTCACGATCCCCAGG
  TCCTTGGATGGACGTCTGCAAGTTTCTCACAGGAAAGGCTTGCCCCATGTTATATATTGCCGTGTTTGGC
  GCTGGCCAGATTTGCAGAGCCATCACGAGCTAAAACCATTGGATATTTGTGAATTTCCTTTTGGATCTAA
  GCAAAAGGAAGTTTGTATCAATCCATACCACTATAAGAGAGTGGAGAGTCCAGTCTTACCTCCAGTATTA
  GTGCCTCGTCACAATGAATTCAATCCACAACACAGCCTTCTGGTTCAGTTCAGGAACCTGAGCCACAATG
  AACCGCACATGCCACAAAACGCCACGTTTCCCGATTCTTTCCACCAACCCAACAACGCTCCTTTCCCCTT
  ATCTCCTAACAGCCCCTATCCTCCTTCCCCTGCTAGCAGCACATATCCCAACTCCCCAGCAAGCTCTGGA
  CCTGGAAGTCCATTTCAACTCCCAGCTGACACCCCTCCCCCTGCCTATATGCCACCTGATGATCAGATGG
  CCCCAGATAATTCCCAGCCTATGGATACAAGCAGTAACATGATTCCTCAGACCATGCCCAGCATATCCAG
  CAGAGATGTTCAGCCTGTCGCCTATGAGGAGCCCAAACACTGGTGTTCGATTGTCTACTATGAATTAAAC
  AATCGTGTTGGGGAAGCTTTTCATGCATCTTCTACTAGTGTGTTAGTAGATGGATTTACAGATCCTTCAA
  ATAACAAAAGTAGATTCTGCCTGGGATTGTTGTCAAATGTTAATCGTAATTCAACTATTGAAAACACTAG
  GCGGCATATTGGAAAAGGTGTTCATCTATACTACGTTGGTGGGGAGGTGTACGCTGAGTGTCTTAGTGAC
  AGCAGCATCTTTGTTCAGAGTAGGAACTGCAACTTTCACCATGGCTTCCATCCCACCACCGTCTGTAAGA
  TCCCCAGCAGCTGCAGCCTCAAGATTTTTAACAATCAGGAGTTTGCTCAGCTTCTGGCTCAGTCAGTCAA
  CCATGGATTCGAGGCTGTGTATGAGCTCACCAAGATGTGTACCATTCGAATGAGCTTTGTCAAGGGCTGG
  GGAGCAGAGTACCACCGACAGGACGTCACCAGTACTCCCTGCTGGATTGAGATTCACCTCCACGGGCCTC
  TGCAGTGGCTGGATAAAGTCCTTACTCAGATGGGCTCTCCGCTGAACCCCATTTCTTCTGTTTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207430 representing BC050001
  Red=Cloning site Green=Tags(s)
  
  MTSMASLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSSPGQPSKCVTIPR
  SLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLDICEFPFGSKQKEVCINPYHYKRVESPVLPPVL
  VPRHNEFNPQHSLLVQFRNLSHNEPHMPQNATFPDSFHQPNNAPFPLSPNSPYPPSPASSTYPNSPASSG
  PGSPFQLPADTPPPAYMPPDDQMAPDNSQPMDTSSNMIPQTMPSISSRDVQPVAYEEPKHWCSIVYYELN
  NRVGEAFHASSTSVLVDGFTDPSNNKSRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSD
  SSIFVQSRNCNFHHGFHPTTVCKIPSSCSLKIFNNQEFAQLLAQSVNHGFEAVYELTKMCTIRMSFVKGW
  GAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPLNPISSVS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC050001
• ORF Size: 1397 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC050001, AAH50001
• RefSeq Size: 6504
• RefSeq ORF: 1397
• Locus ID: 17129