Cap1 (BC005446) Mouse Tagged ORF Clone

CAT#: MG207594

Cap1 (GFP-tagged) - Mouse CAP, adenylate cyclase-associated protein 1 (yeast) (cDNA clone MGC:6142 IMAGE:3600514)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cap1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207594 representing BC005446
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACATGCAAAATCTTGTAGAAAGATTGGAGAGGGCAGTGGGCCGCCTGGAGGCAGTGTCACATA
  CTTCAGACATGCACTGTGGATATGGAGACAGCCCTTCAAAAGGAGCAGTTCCATATGTGCAAGCATTTGA
  CTCGCTGCTTGCCAATCCCGTGGCAGAGTACTTGAAGATGAGTAAGGAGATCGGGGGAGATGTGCAGAAA
  CACGCGGAGATGGTCCACACAGGCCTGAAGTTGGAGCGAGCTCTCCTGGCTACAGCTTCTCAGTGCCAGC
  AGCCAGCTGGTAATAAACTTTCTGATTTGTTGGCACCTATCTCGGAGCAGATCCAAGAAGTTATAACCTT
  CCGGGAGAAGAACCGAGGCAGCAAGTTTTTTAATCATCTATCTGCTGTCAGTGAAAGCATCCAGGCCTTG
  GGCTGGGTGGCTCTGGCTGCGAAACCTGGCCCCTTTGTGAAAGAGATGAATGACGCGGCCATGTTTTACA
  CAAATCGTGTCCTCAAGGAGTACAGAGATGTGGATAAGAAGCATGTGGACTGGGTCAGAGCTTACTTGAG
  TATATGGACGGAGCTGCAGGCTTACATCAAGGAGTTTCATACTACTGGCCTGGCCTGGAGCAAGACGGGG
  CCTGTGGCAAAAGAACTGAGTGGATTGCCATCTGGACCCTCTGTGGGATCAGGCCCACCTCCTCCCCCAC
  CGGGCCCGCCTCCTCCCCCAATTCCTACCAGTTCTGGTTCTGACGACTCTGCATCACGCTCAGCACTGTT
  TGCACAGATTAATCAGGGGGAAAGCATCACACATGCCCTGAAACATGTATCTGATGACATGAAGACTCAC
  AAGAACCCTGCCCTGAAAGCTCAGAGCGGTCCAGTTCGGAGTGGCCCCAAACCATTCTCTGCACCTAAAC
  CCCAAACTAGCCCCTCCCCCAAACCAGCCACAAAGAAGGAACCAGCTCTGCTGGAACTGGAAGGCAAGAA
  ATGGAGAGTGGAAAACCAGGAGAATGTTTCTAACCTGGTGATTGATGACACTGAGCTGAAGCAGGTGGCT
  TACATCTACAAGTGTGTCAACACAACATTGCAAATCAAGGGCAAAATTAACTCCATTACAGTAGATAACT
  GTAAGAAGCTTGGCCTGGTGTTTGATGACGTGGTGGGCATTGTGGAGATAATCAATAGTAGGGATGTCAA
  AGTTCAGGTGATGGGAAAAGTGCCAACCATTTCCATTAACAAAACAGATGGCTGCCATGCTTACCTGAGC
  AAGAACTCCCTGGACTGTGAGATAGTCAGTGCCAAATCTTCTGAGATGAATGTCCTCATTCCTACCGAAG
  GCGGTGATTTTAACGAGTTCCCAGTCCCCGAGCAGTTCAAGACCCTGTGGAACGGACAGAAGTTGGTCAC
  CACAGTGACAGAAATCGCTGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207594 representing BC005446
  Red=Cloning site Green=Tags(s)
  
  MADMQNLVERLERAVGRLEAVSHTSDMHCGYGDSPSKGAVPYVQAFDSLLANPVAEYLKMSKEIGGDVQK
  HAEMVHTGLKLERALLATASQCQQPAGNKLSDLLAPISEQIQEVITFREKNRGSKFFNHLSAVSESIQAL
  GWVALAAKPGPFVKEMNDAAMFYTNRVLKEYRDVDKKHVDWVRAYLSIWTELQAYIKEFHTTGLAWSKTG
  PVAKELSGLPSGPSVGSGPPPPPPGPPPPPIPTSSGSDDSASRSALFAQINQGESITHALKHVSDDMKTH
  KNPALKAQSGPVRSGPKPFSAPKPQTSPSPKPATKKEPALLELEGKKWRVENQENVSNLVIDDTELKQVA
  YIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSRDVKVQVMGKVPTISINKTDGCHAYLS
  KNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005446
• ORF Size: 1424 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC005446, AAH05446
• RefSeq Size: 2543
• RefSeq ORF: 1424
• Locus ID: 12331
• Gene Summary: The product of this gene plays a role in regulating actin dynamics by binding actin monomers and promoting the turnover of actin filaments. Reduced expression of this gene causes a reduction in actin filament turnover rates, causing multiple defects, including an increase in cell size, stress-fiber alterations, and defects in endocytosis and cell motility. A pseudogene of this gene is found on chromosome 14. Alternative splicing results in multiple transcript variants, but does not affect the protein. [provided by RefSeq, Jul 2014]