Chek1 (NM_007691) Mouse Tagged ORF Clone

CAT#: MG207630

Chek1 (GFP-tagged) - Mouse checkpoint kinase 1 homolog (S. pombe) (Chek1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chek1
• Synonyms: C85740; Chk1; rad27
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207630 representing NM_007691
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGCCTTTTGTGGAAGACTGGGATTTGGTGCAAACTTTGGGAGAAGGTGCCTATGGAGAAGTTC
  AACTTGCTGTGAATAGAATAACTGAAGAAGCTGTTGCAGTGAAAATTGTAGACATGAAGCGGGCCATAGA
  CTGTCCAGAAAATATTAAGAAAGAGATCTGCATCAATAAAATGTTAAGCCACGAGAATGTAGTGAAATTC
  TATGGCCACAGGAGGGAAGGCCATATCCAGTATCTGTTTCTGGAGTACTGTAGTGGAGGAGAACTTTTTG
  ATAGAATTGAGCCAGACATAGGGATGCCTGAACAAGATGCTCAGAGGTTCTTCCACCAACTCATGGCAGG
  GGTGGTTTATCTTCATGGAATTGGAATAACTCACAGGGATATTAAACCAGAAAACCTCCTCTTGGATGAA
  AGGGATAACCTCAAAATCTCTGACTTTGGCTTGGCAACGGTATTTCGGCATAATAATCGTGAACGCTTAC
  TGAACAAGATGTGTGGGACTTTACCTTATGTTGCTCCGGAGCTTCTAAAGAGAAAAGAATTTCATGCAGA
  ACCAGTTGATGTTTGGTCCTGTGGAATAGTACTTACTGCAATGTTGGCTGGAGAATTGCCGTGGGACCAG
  CCCAGTGATAGCTGTCAGGAATATTCTGATTGGAAAGAAAAAAAAACCTATCTCAATCCTTGGAAAAAAA
  TTGATTCTGCTCCTCTGGCTTTGCTTCATAAAATTCTAGTTGAGACTCCATCAGCAAGGATCACCATCCC
  AGACATTAAGAAAGATAGATGGTACAACAAACCACTTAACAGAGGAGCAAAGAGGCCACGCGCCACATCA
  GGTGGTATGTCAGAGTCTTCTAGTGGATTCTCTAAGCACATTCATTCCAATTTGGACTTTTCTCCAGTAA
  ATAATGGTTCCAGTGAAGAAACCGTGAAGTTCTCTAGTTCCCAGCCAGAGCCGAGAACAGGGCTTTCCTT
  GTGGGACACTGGTCCCTCGAACGTGGACAAACTGGTTCAGGGCATCAGTTTTTCCCAGCCTACGTGTCCT
  GAGCATATGCTTGTAAACAGTCAGTTACTCGGTACCCCTGGATCTTCACAGAACCCCTGGCAGCGCTTGG
  TCAAAAGGATGACACGATTCTTTACTAAATTGGATGCGGACAAATCTTACCAATGCCTGAAAGAGACCTT
  CGAGAAGTTGGGCTATCAGTGGAAGAAGAGTTGTATGAATCAGGTTACTGTATCAACAACTGATAGAAGA
  AACAATAAGTTGATTTTCAAAATAAATTTGGTAGAAATGGATGAGAAGATACTGGTTGACTTCCGACTTT
  CTAAGGGTGATGGATTAGAGTTCAAGAGACACTTCCTGAAGATTAAAGGGAAGCTCAGCGATGTTGTGAG
  CAGCCAGAAGGTTTGGTTTCCTGTTACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207630 representing NM_007691
  Red=Cloning site Green=Tags(s)
  
  MAVPFVEDWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLSHENVVKF
  YGHRREGHIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDE
  RDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQ
  PSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVETPSARITIPDIKKDRWYNKPLNRGAKRPRATS
  GGMSESSSGFSKHIHSNLDFSPVNNGSSEETVKFSSSQPEPRTGLSLWDTGPSNVDKLVQGISFSQPTCP
  EHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTTDRR
  NNKLIFKINLVEMDEKILVDFRLSKGDGLEFKRHFLKIKGKLSDVVSSQKVWFPVT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007691
• ORF Size: 1428 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007691.5, NP_031717.2
• RefSeq Size: 3397
• RefSeq ORF: 1431
• Locus ID: 12649