Casp8 (NM_009812) Mouse Tagged ORF Clone

CAT#: MG207700

Casp8 (GFP-tagged) - Mouse caspase 8 (Casp8), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Casp8
• Synonyms: CASP-8; FLICE; MACH; Mch5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207700 representing NM_009812
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCCAGAGTTGTCTTTATGCTATTGCTGAAGAACTGGGCAGTGAAGACCTGGCTGCCCTCAAGT
  TCCTGTGCTTGGACTACATCCCACACAAGAAGCAGGAGACCATCGAGGATGCCCAGAAGCTATTTCTGAG
  GCTGCGGGAAAAGGGGATGTTGGAGGAAGGCAATCTGTCTTTCCTGAAAGAGCTGCTTTTCCACATCAGT
  CGGTGGGACCTGCTGGTCAACTTCCTAGACTGCAACCGAGAGGAGATGGTGAGAGAGCTGCGGGATCCAG
  ACAATGCCCAGATTTCTCCCTACAGGGTCATGCTCTTTAAGCTCTCAGAAGAAGTGAGCGAGTTGGAATT
  GAGATCTTTTAAGTTCCTTTTGAACAATGAGATCCCCAAATGTAAGCTGGAAGATGACTTGAGCCTGCTT
  GAAATTTTTGTAGAAATGGAGAAGAGGACCATGCTGGCAGAAAATAACTTGGAAACCCTAAAATCAATCT
  GTGACCAGGTCAACAAGAGCCTGCTGGGGAAGATCGAGGATTATGAAAGATCAAGCACAGAGAGAAGAAT
  GAGCCTTGAAGGAAGGGAAGAGTTGCCACCTTCAGTTTTGGATGAGATGAGCCTCAAAATGGCGGAACTG
  TGTGACTCGCCAAGAGAACAAGACAGTGAGTCACGGACTTCAGACAAAGTTTACCAAATGAAGAACAAAC
  CTCGGGGATACTGTCTGATCATCAACAATCATGATTTCAGCAAGGCCCGGGAAGACATAACCCAACTCCG
  AAAAATGAAGGACAGAAAAGGAACAGACTGTGATAAAGAGGCTCTGAGTAAGACCTTTAAGGAGCTTCAT
  TTTGAGATAGTATCTTACGACGACTGCACTGCAAATGAAATCCACGAGATTCTAGAAGGCTACCAAAGCG
  CAGACCACAAGAACAAAGACTGCTTCATCTGCTGTATCCTATCCCACGGTGACAAGGGTGTCGTCTATGG
  AACGGATGGGAAGGAGGCCTCCATCTATGACCTGACATCTTACTTCACTGGTTCAAAGTGCCCTTCCCTG
  TCTGGGAAACCCAAGATCTTTTTCATTCAGGCTTGCCAAGGAAGTAACTTCCAGAAAGGAGTGCCTGATG
  AGGCAGGCTTCGAGCAACAGAACCACACTTTAGAAGTGGATTCATCATCTCACAAGAACTATATTCCGGA
  TGAGGCAGACTTTCTGCTGGGAATGGCTACGGTGAAGAACTGCGTTTCCTACCGAGATCCTGTGAATGGA
  ACCTGGTATATTCAGTCACTTTGCCAGAGCCTGAGGGAAAGATGTCCTCAAGGAGATGACATTCTTAGCA
  TCCTGACTGGCGTGAACTATGACGTGAGCAATAAAGACGACAGGAGGAACAAGGGAAAGCAGATGCCACA
  GCCCACCTTCACACTACGGAAGAAGCTCTTCTTCCCTCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207700 representing NM_009812
  Red=Cloning site Green=Tags(s)
  
  MDFQSCLYAIAEELGSEDLAALKFLCLDYIPHKKQETIEDAQKLFLRLREKGMLEEGNLSFLKELLFHIS
  RWDLLVNFLDCNREEMVRELRDPDNAQISPYRVMLFKLSEEVSELELRSFKFLLNNEIPKCKLEDDLSLL
  EIFVEMEKRTMLAENNLETLKSICDQVNKSLLGKIEDYERSSTERRMSLEGREELPPSVLDEMSLKMAEL
  CDSPREQDSESRTSDKVYQMKNKPRGYCLIINNHDFSKAREDITQLRKMKDRKGTDCDKEALSKTFKELH
  FEIVSYDDCTANEIHEILEGYQSADHKNKDCFICCILSHGDKGVVYGTDGKEASIYDLTSYFTGSKCPSL
  SGKPKIFFIQACQGSNFQKGVPDEAGFEQQNHTLEVDSSSHKNYIPDEADFLLGMATVKNCVSYRDPVNG
  TWYIQSLCQSLRERCPQGDDILSILTGVNYDVSNKDDRRNKGKQMPQPTFTLRKKLFFPP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009812
• ORF Size: 1440 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009812.2
• RefSeq Size: 2585
• RefSeq ORF: 1443
• Locus ID: 12370
• Gene Summary: This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, an initiator of apoptotic cell death, is activated by death-inducing tumor necrosis family receptors and targets downstream effectors. In mouse deficiency of this gene can cause embryonic lethality. This protein may have a role in embryogenesis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]