F10 (NM_007972) Mouse Tagged ORF Clone

CAT#: MG207705

F10 (GFP-tagged) - Mouse coagulation factor X (F10)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: F10
• Synonyms: Cf10; fX
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207705 representing NM_007972
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGCCCGGTGCAACTCAGCCTGCTCTGTGTTGTCCTGGCCAGCCTCCTGCTCCCTGGGAAAGGTG
  TGTTTATTAACCGGGAACGTGCCAACAATGTCCTGGCGAGGACTCGGAGGGCAAACTCATTTTTTGAAGA
  GTTCAAGAAAGGAAATCTGGAAAGAGAGTGTATGGAAGAAATTTGTTCTTATGAAGAGGTCCGTGAAATC
  TTCGAGGACGACGAGAAGACGAAAGAATACTGGACCAAATATAAAGACGGCGACCAGTGTGAAAGCAGCC
  CTTGCCAGAACCAAGGAGCGTGTCGAGATGGCATCGGGGGTTACACGTGCACCTGCTCGGAGGGATTTGA
  AGGAAAAAACTGTGAGCTCTTTGTTCGGAAACTCTGCCGCCTAGACAACGGAGACTGTGACCAGTTCTGC
  AGAGAAGAGCAGAACTCAGTGGTGTGCTCCTGCGCCAGCGGTTACTTCCTGGGTAATGATGGCAAGTCCT
  GCATCTCCACAGCTCCCTTCCCCTGTGGAAAAATCACTACAGGACGTAGGAAGAGGTCTGTGGCCCTAAA
  CACCAGCGACAGTGAGCTTGACCTTGAAGACGCCCTGCTTGATGAGGATTTCCTGTCCCCTACGGAGAAT
  CCTATTGAACTGCTCAACCTCAACGAGACACAGCCTGAGAGGAGCAGCGATGACCTTGTTCGGATTGTGG
  GTGGCCGGGAATGCAAGGATGGAGAATGTCCCTGGCAGGCTCTGCTCGTTAACGAAGACAATGAAGGGTT
  CTGTGGGGGCACCATCTTGAATGAGTTCTACATCCTCACTGCTGCCCACTGTCTCCATCAGGCCAGGCGA
  TTCAAGGTGAGGGTAGGTGATCGGAACACAGAGAAGGAAGAAGGCAACGAGATGGTGCACGAGGTGGACG
  TGGTCATTAAGCACAACAAGTTTCAGAGGGACACCTACGACTATGATATCGCCGTGCTGAGGCTGAAGAC
  TCCCATCACGTTCCGGATGAACGTGGCCCCTGCCTGCCTGCCTCAGAAAGACTGGGCCGAGTCCACACTG
  ATGACACAGAAGACGGGCATCGTGAGCGGGTTTGGACGCACGCATGAGAAGGGCCGCCAGTCGAACATCC
  TGAAGATGCTGGAGGTACCCTACGTGGATCGCAACACCTGCAAGCTCTCCACCAGCTTCAGCATCACACA
  GAATATGTTCTGTGCGGGCTATGAGGCCAAGTTAGAGGATGCCTGCCAGGGGGACAGTGGTGGCCCCCAT
  GTCACACGGTTCAAAAACACCTACTATGTGACCGGCATTGTCAGCTGGGGAGAGGGGTGTGCAAGGAAAG
  GGAAATATGGCATCTACACAAAGGTCACGACCTTCCTCAAGTGGATTGACAGGTCCATGAAAGCCAGGGT
  GGGACCCACAGCCGAGACCCCAAGGACAGCAGGTCCGCCCAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207705 representing NM_007972
  Red=Cloning site Green=Tags(s)
  
  MGSPVQLSLLCVVLASLLLPGKGVFINRERANNVLARTRRANSFFEEFKKGNLERECMEEICSYEEVREI
  FEDDEKTKEYWTKYKDGDQCESSPCQNQGACRDGIGGYTCTCSEGFEGKNCELFVRKLCRLDNGDCDQFC
  REEQNSVVCSCASGYFLGNDGKSCISTAPFPCGKITTGRRKRSVALNTSDSELDLEDALLDEDFLSPTEN
  PIELLNLNETQPERSSDDLVRIVGGRECKDGECPWQALLVNEDNEGFCGGTILNEFYILTAAHCLHQARR
  FKVRVGDRNTEKEEGNEMVHEVDVVIKHNKFQRDTYDYDIAVLRLKTPITFRMNVAPACLPQKDWAESTL
  MTQKTGIVSGFGRTHEKGRQSNILKMLEVPYVDRNTCKLSTSFSITQNMFCAGYEAKLEDACQGDSGGPH
  VTRFKNTYYVTGIVSWGEGCARKGKYGIYTKVTTFLKWIDRSMKARVGPTAETPRTAGPPN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007972
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007972.3, NP_031998.3
• RefSeq Size: 2043
• RefSeq ORF: 1446
• Locus ID: 14058
• Gene Summary: This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]