Nle1 (NM_145431) Mouse Tagged ORF Clone

CAT#: MG207771

Nle1 (GFP-tagged) - Mouse notchless homolog 1 (Drosophila) (Nle1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nle1
• Synonyms: AL022765; BC018399; l11Jus1; l11Jus4; Nle
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207771 representing NM_145431
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGTGGTGGAAGAGGCGGCGGCAGGCGACGTGCAGCGGCTGCTCGTACAGTTCCAGGATG
  AGGGCGGTCAGCTGCTGGGCTCCCCGTTCGACGTGCCCGTGGACATCACGCCGGACAAGCTGCAGCTGGT
  GTGCAACGCGCTGCTGGCCCAGGAGGAGCCCCTGCCCCTGGCGTTCTATGTCCACGATGCTGAGATTGTG
  TCCTCCCTGGGGAAGACGCTGGAGTCCCAGTCTGTGGAGACAGAGAAAATCGTCGATATCATCTACCAGC
  CCCAAGCTGTCTTCAGGGTCCGGGCTGTGACGCGGTGCACCAGCTCATTGGAGGGTCACAGCGAAGCTGT
  CATTTCTGTGGCCTTCAGCCCCACAGGAAAGTACCTGGCCAGTGGTTCCGGAGACACCACTGTGCGCTTC
  TGGGATCTCAGCACTGAGACACCGCACTTCACATGCAAAGGACACCGGCACTGGGTCCTTAGCATATCCT
  GGTCCCCAGATGGCAAGAAACTGGCCTCAGGCTGCAAGAATGGCCAGATTCTGCTGTGGGACCCAAGCAC
  GGGGCTGCAGGTGGGCAGGACCCTCACTGGCCACAGCAAATGGATCACAGGCCTGAGCTGGGAGCCCCTT
  CACATGAACCCCGAGTGCCGCTACGTGGCCAGCAGCTCCAAAGACGGCAGTGTACGAGTCTGGGATACAA
  CCGCAGGCCGCTGTGAACGCATCCTCACGGGACACACGCAGTCAGTCACCTGCCTCCGATGGGGAGGAGA
  TGGGCTTCTATATTCTGCTTCCCAGGACCGAACCATCAAAGTCTGGAGGGCCCATGACGGTGTGCTGTGT
  CGTACTCTTCAAGGTCATGGCCACTGGGTGAACACCATGGCGCTTAGCACAGACTATGCCCTGCGCACAG
  GGGCCTTTGAGCCTGCTGAAGCCACAGTGAATGCCCAAGACCTGCAGGGGTCCTTGAAGGAGCTGAAGGA
  GAGGGCATCGAGCCGCTACAACCTCGTGCGGGGCCAAGGCCCAGAGAGGCTGGTCTCTGGCTCAGACGAC
  TTCACCTTATTCCTGTGGTCCCCAGCAGAGGACAAGAAGCCCCTCGCCCGGATGACAGGACACCAGGCCC
  TCATCAACCAGGTGCTCTTCTCTCCTGACTCGCGCATCGTGGCCAGTGCCTCCTTCGACAAGTCTATCAA
  GCTGTGGGATGGCCGGACGGGCAAGTACCTAGCTTCCCTGCGTGGCCACGTGGCAGCCGTGTACCAGATT
  GCCTGGTCAGCTGACAGCCGACTATTAGTCAGCGGCAGCAGTGACAGCACGCTGAAGGTGTGGGATGTGA
  AGGCCCAGAAACTGGCCACGGACCTGCCTGGCCATGCCGATGAGGTATATGCTGTTGACTGGAGTCCAGA
  TGGCCAGAGAGTAGCAAGCGGCGGGAAAGACAAGTGCCTCCGGATATGGAGAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207771 representing NM_145431
  Red=Cloning site Green=Tags(s)
  
  MAAAVVEEAAAGDVQRLLVQFQDEGGQLLGSPFDVPVDITPDKLQLVCNALLAQEEPLPLAFYVHDAEIV
  SSLGKTLESQSVETEKIVDIIYQPQAVFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRF
  WDLSTETPHFTCKGHRHWVLSISWSPDGKKLASGCKNGQILLWDPSTGLQVGRTLTGHSKWITGLSWEPL
  HMNPECRYVASSSKDGSVRVWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLC
  RTLQGHGHWVNTMALSTDYALRTGAFEPAEATVNAQDLQGSLKELKERASSRYNLVRGQGPERLVSGSDD
  FTLFLWSPAEDKKPLARMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQI
  AWSADSRLLVSGSSDSTLKVWDVKAQKLATDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145431
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145431.1, NP_663406.1
• RefSeq Size: 1784
• RefSeq ORF: 1458
• Locus ID: 217011