Hcls1 (NM_008225) Mouse Tagged ORF Clone

CAT#: MG207789

Hcls1 (GFP-tagged) - Mouse hematopoietic cell specific Lyn substrate 1 (Hcls1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hcls1
• Synonyms: AW213261; HS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207789 representing NM_008225
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAAGTCTGTAGTGGGGCATGATGTATCGGTTTCCGTGGAGACCCAGGGTGATGACTGGGATACAG
  ACCCTGACTTTGTGAATGACATCTCCGAGAAGGAGCAACGGTGGGGAGCCAAGACCATTGAGGGCTCTGG
  ACGCACAGAGCACATCAACATCCACCAGCTGAGGAACAAAGTGTCAGAGGAGCACGACATCCTCAAGAAG
  AAGGAGCTGGAATCGGGGCCTAAGGCATCCCATGGCTATGGCGGTCGGTTTGGAGTGGAGAGAGACCGGA
  TGGACAAGAGTGCCGTGGGCCACGAGTATGTTGCTGATGTGGAGAAACACTCATCTCAGACTGATGCCGC
  CAGAGGCTTTGGGGGCAAATATGGAGTTGAGAGGGACCGGGCAGACAAGTCAGCGGTGGGCTTTGACTAC
  AAAGGAGAAGTGGAAAAGCATGCATCTCAGAAAGATTACTCTCATGGCTTTGGTGGCCGCTACGGGGTAG
  AGAAGGATAAACGGGACAAAGCAGCCCTGGGATACGACTACAAAGGAGAGACGGAGAAGCACGAGTCTCA
  GAGAGATTATGCCAAGGGCTTTGGTGGCCAATATGGAATCCAGAAAGACCGAGTGGATAAGAGTGCTGTT
  GGCTTCAATGAAATGGAGGCCCCAACCACGGCGTATAAGAAGACAACACCCATAGAAGCTGCTTCCAGTG
  GTGCCCGTGGGCTGAAGGCAAAATTTGAGTCCCTGGCTGAGGAGAAGAGGAAGCGAGAGGAAGAAGAGAA
  GGCACAGCAGATGGCCAGGCAGCAACAGGAGCGAAAGGCTGTGGTAAAGATGAGCCGAGAAGTCCAGCAG
  CCATCCATGCCTGTGGAAGAGCCAGCGGCACCAGCCCAGTTGCCCAAGAAGATCTCCTCAGAGGTCTGGC
  CTCCAGCAGAGAGTCACCTACCGCCAGAGTCTCAGCCAGTGAGAAGCAGAAGGGAATACCCTGTGCCCTC
  TCTGCCCACGAGGCAGTCTCCATTGCAGAATCACTTGGAGGACAACGAGGAGCCCCCAGCTCTGCCCCCT
  AGGACCCCAGAAGGCCTCCAGGTGGTGGAAGAGCCAGTGTACGAAGCAGCACCCGAGCTGGAGCCGGAGC
  CAGAGCCTGACTATGAGCCAGAGCCAGAGACAGAGCCTGACTATGAGGATGTTGGGGAGTTAGATCGGCA
  GGATGAGGATGCAGAGGGAGACTATGAGGATGTGCTGGAGCCCGAGGACACCCCTTCTCTGTCCTACCAA
  GCTGGACCCTCAGCTGGGGCTGGTGGTGCGGGGATCTCTGCTATAGCCCTGTATGATTACCAAGGAGAGG
  GAAGCGATGAGCTTTCCTTTGATCCAGATGACATCATCACTGACATTGAGATGGTGGATGAAGGCTGGTG
  GCGGGGCCAATGCCGTGGCCACTTTGGACTTTTCCCTGCAAACTATGTCAAGCTCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207789 representing NM_008225
  Red=Cloning site Green=Tags(s)
  
  MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDILKK
  KELESGPKASHGYGGRFGVERDRMDKSAVGHEYVADVEKHSSQTDAARGFGGKYGVERDRADKSAVGFDY
  KGEVEKHASQKDYSHGFGGRYGVEKDKRDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAV
  GFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESLAEEKRKREEEEKAQQMARQQQERKAVVKMSREVQQ
  PSMPVEEPAAPAQLPKKISSEVWPPAESHLPPESQPVRSRREYPVPSLPTRQSPLQNHLEDNEEPPALPP
  RTPEGLQVVEEPVYEAAPELEPEPEPDYEPEPETEPDYEDVGELDRQDEDAEGDYEDVLEPEDTPSLSYQ
  AGPSAGAGGAGISAIALYDYQGEGSDELSFDPDDIITDIEMVDEGWWRGQCRGHFGLFPANYVKLL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008225
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008225.1, NP_032251.1
• RefSeq Size: 2003
• RefSeq ORF: 1461
• Locus ID: 15163