Hcls1 (NM_008225) Mouse Tagged ORF Clone

CAT#: MR207789

Hcls1 (Myc-DDK-tagged) - Mouse hematopoietic cell specific Lyn substrate 1 (Hcls1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hcls1
• Synonyms: AW213261; HS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207789 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAAGTCTGTAGTGGGGCATGATGTATCGGTTTCCGTGGAGACCCAGGGTGATGACTGGGATACAG
  ACCCTGACTTTGTGAATGACATCTCCGAGAAGGAGCAACGGTGGGGAGCCAAGACCATTGAGGGCTCTGG
  ACGCACAGAGCACATCAACATCCACCAGCTGAGGAACAAAGTGTCAGAGGAGCACGACATCCTCAAGAAG
  AAGGAGCTGGAATCGGGGCCTAAGGCATCCCATGGCTATGGCGGTCGGTTTGGAGTGGAGAGAGACCGGA
  TGGACAAGAGTGCCGTGGGCCACGAGTATGTTGCTGATGTGGAGAAACACTCATCTCAGACTGATGCCGC
  CAGAGGCTTTGGGGGCAAATATGGAGTTGAGAGGGACCGGGCAGACAAGTCAGCGGTGGGCTTTGACTAC
  AAAGGAGAAGTGGAAAAGCATGCATCTCAGAAAGATTACTCTCATGGCTTTGGTGGCCGCTACGGGGTAG
  AGAAGGATAAACGGGACAAAGCAGCCCTGGGATACGACTACAAAGGAGAGACGGAGAAGCACGAGTCTCA
  GAGAGATTATGCCAAGGGCTTTGGTGGCCAATATGGAATCCAGAAAGACCGAGTGGATAAGAGTGCTGTT
  GGCTTCAATGAAATGGAGGCCCCAACCACGGCGTATAAGAAGACAACACCCATAGAAGCTGCTTCCAGTG
  GTGCCCGTGGGCTGAAGGCAAAATTTGAGTCCCTGGCTGAGGAGAAGAGGAAGCGAGAGGAAGAAGAGAA
  GGCACAGCAGATGGCCAGGCAGCAACAGGAGCGAAAGGCTGTGGTAAAGATGAGCCGAGAAGTCCAGCAG
  CCATCCATGCCTGTGGAAGAGCCAGCGGCACCAGCCCAGTTGCCCAAGAAGATCTCCTCAGAGGTCTGGC
  CTCCAGCAGAGAGTCACCTACCGCCAGAGTCTCAGCCAGTGAGAAGCAGAAGGGAATACCCTGTGCCCTC
  TCTGCCCACGAGGCAGTCTCCATTGCAGAATCACTTGGAGGACAACGAGGAGCCCCCAGCTCTGCCCCCT
  AGGACCCCAGAAGGCCTCCAGGTGGTGGAAGAGCCAGTGTACGAAGCAGCACCCGAGCTGGAGCCGGAGC
  CAGAGCCTGACTATGAGCCAGAGCCAGAGACAGAGCCTGACTATGAGGATGTTGGGGAGTTAGATCGGCA
  GGATGAGGATGCAGAGGGAGACTATGAGGATGTGCTGGAGCCCGAGGACACCCCTTCTCTGTCCTACCAA
  GCTGGACCCTCAGCTGGGGCTGGTGGTGCGGGGATCTCTGCTATAGCCCTGTATGATTACCAAGGAGAGG
  GAAGCGATGAGCTTTCCTTTGATCCAGATGACATCATCACTGACATTGAGATGGTGGATGAAGGCTGGTG
  GCGGGGCCAATGCCGTGGCCACTTTGGACTTTTCCCTGCAAACTATGTCAAGCTCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207789 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDILKK
  KELESGPKASHGYGGRFGVERDRMDKSAVGHEYVADVEKHSSQTDAARGFGGKYGVERDRADKSAVGFDY
  KGEVEKHASQKDYSHGFGGRYGVEKDKRDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAV
  GFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESLAEEKRKREEEEKAQQMARQQQERKAVVKMSREVQQ
  PSMPVEEPAAPAQLPKKISSEVWPPAESHLPPESQPVRSRREYPVPSLPTRQSPLQNHLEDNEEPPALPP
  RTPEGLQVVEEPVYEAAPELEPEPEPDYEPEPETEPDYEDVGELDRQDEDAEGDYEDVLEPEDTPSLSYQ
  AGPSAGAGGAGISAIALYDYQGEGSDELSFDPDDIITDIEMVDEGWWRGQCRGHFGLFPANYVKLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008225
• ORF Size: 1461 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008225.1, NM_008225.2, NP_032251.1, NP_032251.2
• RefSeq Size: 2031 bp
• RefSeq ORF: 1461 bp
• Locus ID: 15163
• MW: 54.2 kDa