Glrb (BC037605) Mouse Tagged ORF Clone

CAT#: MG207969

Glrb (GFP-tagged) - Mouse glycine receptor, beta subunit (cDNA clone MGC:46752 IMAGE:5361798)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Glrb
• Synonyms: spastic, Glyrb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207969 representing BC037605
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTTTCGTTGGCAATCTCCTTTTTTATTTTAATGTCCTTGTTGTTTGAAGACGCTTGTGCTAAAG
  AAAAGTCTTCCAAGAAAGGGAAGGGGAAAAAGAAGCAGTACTTGTGCCCATCTCAGCAGTCACCCGAGGA
  CCTGGCCCGTGTGCCCCCCAACTCCACCAGCAATATCTTGAACAGGCTGCTGGTCAGTTATGATCCCAGG
  ATCAGACCAAACTTCAAAGGCATTCCTGTTGATGTAGTAGTCAACATTTTTATTAATAGTTTTGGATCCA
  TTCAAGAGACAACAATGGACTATAGAGTTAACATTTTCTTGAGACAGAAATGGAATGACCCCAGACTCAA
  GCTACCTAGTGACTTCAGAGGCTCAGATGCACTGACAGTTGACCCCACCATGTATAAGTGCTTGTGGAAA
  CCTGACTTATTCTTTGCAAATGAAAAAAGTGCCAATTTTCATGATGTGACCCAAGAAAATATCCTGTTGT
  TTATCTTTCGGGATGGAGACGTCCTTGTGAGCATGAGGTTGTCTATTACACTTTCATGTCCTCTAGACTT
  AACTCTGTTTCCCATGGACACACAACGCTGCAAAATGCAACTTGAGAGCTTTGGATATACAACCGATGAT
  TTAAGATTCATCTGGCAGTCAGGAGATCCTGTTCAGTTGGAAAAAATTGCTTTACCTCAATTTGATATTA
  AAAAGGAGGATATCGAATATGGCAACTGTACAAAATACTATAAAGGCACTGGTTACTACACTTGTGTGGA
  GGTCATCTTCACCCTGAGGAGACAGGTTGGGTTCTACATGATGGGCGTATATGCACCAACCTTGCTGATT
  GTGGTTCTCTCCTGGCTCTCTTTCTGGATCAACCCTGATGCTAGTGCTGCCAGAGTACCTCTGGGCATCT
  TCTCCGTGCTCAGTTTGGCCTCAGAGTGCACCACCCTCGCAGCCGAGCTTCCTAAAGTGTCTTATGTGAA
  GGCGCTGGATGTGTGGCTCATTGCCTGCCTGCTCTTCGGGTTTGCCTCCCTCGTGGAGTACGCTGTGGTC
  CAGGTGATGCTGAACAATCCCAAAAGGGTTGAAGCCGAGAAGGCCCGAATAGCTAAGGCTGAGCAAGCAG
  ATGGGAAAGGTGGAAACGCAGCTAAGAAGAATACTGTAAACGGCACGGGGACCCCTGTTCATATCAGCAC
  TTTGCAGGTTGGTGAGACCAGATGCAAAAAAGTTTGTACTTCCAAGTCTGATTTGAGATCCAATGACTTC
  AGCATTGTTGGAAGCTTACCAAGAGATTTTGAATTATCCAATTATGACTGCTATGGGAAACCCATCGAAG
  TCAACAATGGACTTGGGAAACCACAGGCAAAGAACAAGAAGCCTCCGCCTGCCAAGCCTGTCATCCCAAC
  AGCAGCCAAGCGCATCGACCTTTATGCAAGAGCATTATTTCCTTTCTGCTTCTTGTTCTTCAATGTTATA
  TATTGGTCTATATATTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207969 representing BC037605
  Red=Cloning site Green=Tags(s)
  
  MKFSLAISFFILMSLLFEDACAKEKSSKKGKGKKKQYLCPSQQSPEDLARVPPNSTSNILNRLLVSYDPR
  IRPNFKGIPVDVVVNIFINSFGSIQETTMDYRVNIFLRQKWNDPRLKLPSDFRGSDALTVDPTMYKCLWK
  PDLFFANEKSANFHDVTQENILLFIFRDGDVLVSMRLSITLSCPLDLTLFPMDTQRCKMQLESFGYTTDD
  LRFIWQSGDPVQLEKIALPQFDIKKEDIEYGNCTKYYKGTGYYTCVEVIFTLRRQVGFYMMGVYAPTLLI
  VVLSWLSFWINPDASAARVPLGIFSVLSLASECTTLAAELPKVSYVKALDVWLIACLLFGFASLVEYAVV
  QVMLNNPKRVEAEKARIAKAEQADGKGGNAAKKNTVNGTGTPVHISTLQVGETRCKKVCTSKSDLRSNDF
  SIVGSLPRDFELSNYDCYGKPIEVNNGLGKPQAKNKKPPPAKPVIPTAAKRIDLYARALFPFCFLFFNVI
  YWSIYL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC037605
• ORF Size: 1490 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC037605, AAH37605
• RefSeq Size: 3008
• RefSeq ORF: 1490
• Locus ID: 14658
• Gene Summary: This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. This gene is transcribed throughout the central nervous system of neonatal and adult mice. In humans, mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]