Ptgis (NM_008968) Mouse Tagged ORF Clone

CAT#: MG208060

Ptgis (GFP-tagged) - Mouse prostaglandin I2 (prostacyclin) synthase (Ptgis)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ptgis
• Synonyms: Cyp8; Cyp8a1; Pgis
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208060 representing NM_008968
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTGGGCTGCTCTGCTCGGCCTCCTGGCCGTGCTATTGCTGTTGCTGCTGCTGCTGAGCCGCCGGC
  GCGCGCGGAGACCTGGTGAGCCTCCGTTGGACCTGGGCAGCATCCCCTGGCTGGGCCATGCCTTGGAGTT
  TGGGAGAGATGCTGCCAGCTTCCTTACCAGGATGAAGGAAAAGCACGGTGACATATTTACTGTGCTGGTG
  GGGGGCAGATACGTCACTGTTCTCCTGGACCCACACTCTTACGACACAGTGGTGTGGGAGCTGCGTACAC
  GGCTGGACTTCCATCCCTATGCCATCTTCCTCATGGAGAGGATTTTTGATCTGCAGCTTCCAAATTTCAA
  CCCCAGTGAGGAGAAGGCCAGGATGAAGCCGACGCTCATGCACAGAGACCTGCAGGCGCTCACAGAAGCC
  ATGTATACCAACCTTCGCACGGTCCTGCTGGGTGACTCCACAGAAGCAGGCAGTGGCTGGCAGGAGACAG
  GTCTCCTTGAGTTCTCCTACAACGCCCTGCTCAGCGCCGGCTACCTGACCCTATATGGAGTGGAGGCCTC
  ACCACGCACCCATGAGAGTCAGGCCCAAGATCGTGTCCACTCAGCGGATGTTTTCCATACCTTCCGCCAG
  CTGGATCTGCTGCTCCCCAAACTGGCTCGAGGCTCCTTGTCAGCGGGGGATAAAGACCATGCATGCAGTG
  TCAAAAACCGCCTGTGGAAGCTGCTGTCCCCAGCCAGGCTGGCCTCCAGGGCTGACCGGAGCAGCTGGCT
  GGAGAGTTATCTGAGACATCTTGAGGAGATGGGGGTGTCAGAGGAGATGCAGGCCCGGGCTCTGGTGTTG
  CAGCTCTGGGCCACACAGGGGAATATGGGTCCCACGGCCTTCTGGCTCCTTCTTTTCCTCCTCAAGAATC
  CGGAAGCCCTCGCCGCTGTCCGTGCAGAGCTGAAGCACACGGTCTGGCAAGCAGAGCAGCCTGTTTCACA
  GATGACCACACTCCCACAGAAGATTCTAGACAGCATGCCTGTGCTAGACAGTGTGCTCAATGAGACACTC
  CGGCTCACGGCTGCCCCCTTCATCACCCGTGAGGTCATGGCAGACCTGGCCTTGCCTATGGCAGACGGGA
  GGGAATTCTCTCTTCGACGTGGTGACCGCCTTCTCCTCTTTCCCTTCCTGAGTCCCCAGAAGGACCCAGA
  AATCTACACAGAGCCTGAGGTGTTTAAATACAACCGATTCTTGAACCCAGATGGATCTGAAAAGAAAGAT
  TTTTACAAAGATGGGAAACGGCTGAAGAATTACAACATGCCATGGGGCGCAGGGCACAACCAGTGCCTGG
  GGAAGAGTTATGCCATCAACAGCATCAAACAATTTGTCGTCCTACTGCTGACTCATTTCGACCTGGAGCT
  GGGCAGCGAGGACACAGAGGTTCCTGAGTTTGACCTCAGCAGATATGGCTTCGGTCTGATGCAGCCAGAG
  GAAGACGTGCCCATCCGGTACCGTGCCAGGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208060 representing NM_008968
  Red=Cloning site Green=Tags(s)
  
  MSWAALLGLLAVLLLLLLLLSRRRARRPGEPPLDLGSIPWLGHALEFGRDAASFLTRMKEKHGDIFTVLV
  GGRYVTVLLDPHSYDTVVWELRTRLDFHPYAIFLMERIFDLQLPNFNPSEEKARMKPTLMHRDLQALTEA
  MYTNLRTVLLGDSTEAGSGWQETGLLEFSYNALLSAGYLTLYGVEASPRTHESQAQDRVHSADVFHTFRQ
  LDLLLPKLARGSLSAGDKDHACSVKNRLWKLLSPARLASRADRSSWLESYLRHLEEMGVSEEMQARALVL
  QLWATQGNMGPTAFWLLLFLLKNPEALAAVRAELKHTVWQAEQPVSQMTTLPQKILDSMPVLDSVLNETL
  RLTAAPFITREVMADLALPMADGREFSLRRGDRLLLFPFLSPQKDPEIYTEPEVFKYNRFLNPDGSEKKD
  FYKDGKRLKNYNMPWGAGHNQCLGKSYAINSIKQFVVLLLTHFDLELGSEDTEVPEFDLSRYGFGLMQPE
  EDVPIRYRARL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008968
• ORF Size: 1503 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008968.3, NP_032994.1
• RefSeq Size: 1751
• RefSeq ORF: 1506
• Locus ID: 19223
• Gene Summary: Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2). [UniProtKB/Swiss-Prot Function]