Plk3 (BC031180) Mouse Tagged ORF Clone

CAT#: MG208102

Plk3 (GFP-tagged) - Mouse polo-like kinase 3 (Drosophila) (cDNA clone MGC:37578 IMAGE:4988360)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plk3
• Synonyms: Fnk, PRK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208102 representing BC031180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGCCGCCGGCTTCTTGTCTCCGCGCCCCTTCCCGCGCGCGGCTGCCCCGTCCGCGCCCCCCG
  CCGGCCCGGGGCCGCCTGCCAATGCCTCCCCGCGATCGGAACCTGAGGTGCTGGCCGGGCCACGGGCGCC
  GGATCCTCCTGGTCGCCTCATCACCGACCCTCTCAGCGGCCGTACCTACACCAAGGGTCGCTTGTTGGGC
  AAGGGGGGCTTCGCACGCTGCTATGAAGCCACTGACACCGAGTCTGGTATAGCCTACGCGGTCAAAGTCA
  TCCCGCAGAGTCGCGTCGCCAAGCCGCATCAGCGCGAGAAGATCCTAAATGAGATAGAGTTGCACCGAGA
  CCTACAGCACCGCCATATCGTTCGCTTTTCACATCATTTCGAGGATGCTGACAACATATACATTTTCCTG
  GAGCTCTGTAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGACACACCCTGTTGGAACCAGAGGTTC
  GCTACTACCTGCGCCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGAGGGGTATCTTACACAGAGATCT
  CAAGCTGGGAAATTTTTTCATCACGGATAACATGGAACTGAAGGTGGGGGATTTTGGGCTGGCAGCTCGA
  TTAGAGCCCCCAGAGCAGAGGAAAAAGACCATCTGTGGCACTCCCAACTACGTGGCTCCAGAAGTGCTGC
  TGAGACAGGGCCATGGCCCCGAGGCAGATGTGTGGTCGCTTGGTTGTGTCATGTACACGCTCCTGTGTGG
  GAGCCCCCCATTTGAGACTGCTGACCTGAAGGAGACATACCGCTGCATCAAGCAGGTTCATTACACGCTA
  CCTGCCAGCCTCTCCCTGCCTGCCCGGCAGCTCCTGGCTGCCATCCTCCGAGCTTCACCCAGAGACCGCC
  CTTCCATTGAACAGATTTTACGCCATGACTTCTTTACGAAGGGCTACACCCCTGATCGGCTCCCAGTCAG
  CAGCTGTGTGACAGTCCCAGATCTGACACCCCCCAACCCTGCGAGGAGTCTGTTTGCCAAAGTTACCAAG
  AGCCTGTTTGGCAGGAAAAAGAACAAGAATAAGAACCATTCTGAGGACCAGGATAATGTCTCCTGCTTGG
  TGAGTGGCCTCATGCGCACATCCATCGGTCATCCAGATGTCAGGCCGGAGGCTCCTGTAGTTTCAGGCCA
  GGCCCCTGCCAGCTTGATAGAGACAGCTGCCGAGGACAGCTCGCCCCGTGGGACACTGGCCAGCAGTGGA
  GATGGGTTTGAAGAAGGTTTGACTGTGGCCACAGTGGTAGAGTCTGCCCTCTGTGCACTGAGAAACTGTG
  TGGCCTTCATGCCCCCAGCCGAACAGAACCCAGCCCCACTGGCACAGCCAGAGCCTCTGGTGTGGGTCAG
  CAAGTGGGTTGACTACTCCAACAAGTTTGGCTTTGGCTATCAGCTGTCCAGCCGCCGTGTAGCCGTGCTC
  TTCAATGATGGCACACACATGGCCCTGTCAGCCAATAGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208102 representing BC031180
  Red=Cloning site Green=Tags(s)
  
  MEPAAGFLSPRPFPRAAAPSAPPAGPGPPANASPRSEPEVLAGPRAPDPPGRLITDPLSGRTYTKGRLLG
  KGGFARCYEATDTESGIAYAVKVIPQSRVAKPHQREKILNEIELHRDLQHRHIVRFSHHFEDADNIYIFL
  ELCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQRGILHRDLKLGNFFITDNMELKVGDFGLAAR
  LEPPEQRKKTICGTPNYVAPEVLLRQGHGPEADVWSLGCVMYTLLCGSPPFETADLKETYRCIKQVHYTL
  PASLSLPARQLLAAILRASPRDRPSIEQILRHDFFTKGYTPDRLPVSSCVTVPDLTPPNPARSLFAKVTK
  SLFGRKKNKNKNHSEDQDNVSCLVSGLMRTSIGHPDVRPEAPVVSGQAPASLIETAAEDSSPRGTLASSG
  DGFEEGLTVATVVESALCALRNCVAFMPPAEQNPAPLAQPEPLVWVSKWVDYSNKFGFGYQLSSRRVAVL
  FNDGTHMALSANRK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031180
• ORF Size: 1514 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC031180, AAH31180
• RefSeq Size: 2648
• RefSeq ORF: 1514
• Locus ID: 12795
• Gene Summary: This gene encodes a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal catalytic domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases have primarily been implicated in cell cycle regulation. In mouse, this protein that has been reported to localize to the nucleolus during interphase but is undetectable during mitosis, following nucleolus dissociation during prophase. The protein relocalizes to the nucleolus just prior to cytokinesis and peak levels are detected during G1 of interphase. This gene has been implicated in regulation of entry into S phase, with RNAi-induced depletion resulting in failure to re-enter the cell cycle. Mice deficient for this gene exhibit increased weight and tumor development at advanced age. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]