Cyp17a1 (NM_007809) Mouse Tagged ORF Clone

CAT#: MG208156

Cyp17a1 (GFP-tagged) - Mouse cytochrome P450, family 17, subfamily a, polypeptide 1 (Cyp17a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyp17a1
• Synonyms: Cyp17; p450c17
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208156 representing NM_007809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGAACTTGTGGGTCTCTTGCTGCTCATCCTGGCCTATTTCTTTTGGCCCAAGTCAAAGACACCTA
  ATGCCAAGTTCCCCAGGAGCCTTCCATTCCTGCCCCTGGTGGGTAGTCTACCGTTTCTCCCCAGACGTGG
  TCATATGCATGCCAACTTCTTCAAGCTGCAGGAAAAGTATGGTCCCATCTATTCTCTTCGCCTGGGTACC
  ACAACTGCAGTGATTGTCGGTCACTATCAGCTGGCCAGAGAAGTGCTCGTGAAGAAGGGGAAAGAATTCT
  CTGGTCGGCCCCAGATGGTGACTCTAGGCCTCTTGTCGGACCAAGGAAAAGGCGTCGCCTTTGCGGATAG
  TAGTAGCTCCTGGCAGCTGCACCGGAAGCTGGTATTCAGCACCTTTTCCCTGTTCAGGGATGACCAGAAA
  CTGGAGAAGATGATATGTCAGGAAGCCAACTCACTGTGTGACTTGATACTTACATACGACGGGGAGTCCC
  GAGATCTGTCTACGCTCATCTTCAAGTCAGTAATCAATATCATCTGTACCATCTGCTTCAACATCTCTTT
  TGAGAACAAGGATCCGATACTGACTACCATACAGACCTTTACAGAGGGTATTGTGGATGTCCTGGGCCAC
  AGCGATCTGGTGGACATATTCCCGTGGTTGAAGATTTTTCCCAATAAAAACTTGGAAATGATAAAGGAAC
  ACACTAAAATTCGAGAAAAAACACTGGTTGAAATGTTTGAAAAATGCAAGGAGAAATTCAATAGTGAATC
  TCTCTCCAGCCTGACAGACATTCTGATACAAGCCAAGATGAATGCAGAAAATAATAACACTGGGGAAGGC
  CAGGACCCAAGTGTGTTCTCAGATAAGCATATCCTTGTCACGGTGGGAGACATCTTTGGGGCAGGCATAG
  AGACAACTAGCTCTGTGCTGAGCTGGATCCTGGCTTTCCTGGTGCACAATCCTGAGGTGAAGAGGAAGAT
  CCAAAAGGAGATTGACCAGTATGTAGGCTTCAGTCGAACACCGTCTTTCAATGACCGGACTCACCTCCTC
  ATGCTGGAGGCCACTATCCGAGAAGTGCTTCGTATCAGGCCGGTGGCCCCCTTGCTCATCCCACACAAGG
  CTAACATTGACTCCAGCATTGGAGAGTTTGCCATCCCGAAGGACACACATGTGATCATCAATCTCTGGGC
  ACTGCATCACGATAAAAATGAATGGGACCAGCCAGATCGGTTTATGCCTGAGCGCTTCTTAGATCCAACA
  GGAAGCCATCTCATTACACCCACACCCAGTTATTTGCCCTTCGGAGCTGGTCCCCGATCGTGCATTGGAG
  AGGCTCTGGCCCGGCAGGAGCTCTTTATCTTCATGGCCTTGCTGCTGCAGAGGTTTGACTTTGATGTGTC
  AGATGACAAACAGCTGCCCTGTCTGGTGGGTGACCCCAAGGTGGTCTTTCTGATCGACCCTTTCAAAGTG
  AAAATCACAGTGCGACAAGCATGGAAGGATGCACAGGTTGAGGTTAGCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208156 representing NM_007809
  Red=Cloning site Green=Tags(s)
  
  MWELVGLLLLILAYFFWPKSKTPNAKFPRSLPFLPLVGSLPFLPRRGHMHANFFKLQEKYGPIYSLRLGT
  TTAVIVGHYQLAREVLVKKGKEFSGRPQMVTLGLLSDQGKGVAFADSSSSWQLHRKLVFSTFSLFRDDQK
  LEKMICQEANSLCDLILTYDGESRDLSTLIFKSVINIICTICFNISFENKDPILTTIQTFTEGIVDVLGH
  SDLVDIFPWLKIFPNKNLEMIKEHTKIREKTLVEMFEKCKEKFNSESLSSLTDILIQAKMNAENNNTGEG
  QDPSVFSDKHILVTVGDIFGAGIETTSSVLSWILAFLVHNPEVKRKIQKEIDQYVGFSRTPSFNDRTHLL
  MLEATIREVLRIRPVAPLLIPHKANIDSSIGEFAIPKDTHVIINLWALHHDKNEWDQPDRFMPERFLDPT
  GSHLITPTPSYLPFGAGPRSCIGEALARQELFIFMALLLQRFDFDVSDDKQLPCLVGDPKVVFLIDPFKV
  KITVRQAWKDAQVEVST
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007809
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007809.2, NP_031835.2
• RefSeq Size: 1813
• RefSeq ORF: 1524
• Locus ID: 13074
• Gene Summary: Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. [UniProtKB/Swiss-Prot Function]