Hmgcs2 (BC014714) Mouse Tagged ORF Clone

CAT#: MG208162

Hmgcs2 (GFP-tagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (cDNA clone MGC:25633 IMAGE:4216637)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hmgcs2
• Synonyms: mHS, 1300002P16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208162 representing BC014714
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGCTTTTGGCTCCAGCGAGGCGGGTCCTGCAAGTGAAGAGAGCGATGCAGGAAACTTCGCTCA
  CACCTGCTCACCTGCTCTCAGCAGCCCAGCAGAGGTTTTCTACAATCCCTCCTGCTCCCCTGGCCAAAAC
  AGATACATGGCCAAAAGATGTGGGCATCCTTGCCCTGGAGGTCTATTTTCCAGCCCAATATGTGGACCAA
  ACTGACCTGGAAAAGTTCAACAATGTGGAAGCAGGGAAGTATACAGTGGGCTTGGGCCAGACCCGTATGG
  GCTTCTGTTCAGTCCAGGAGGACATCAACTCCCTGTGCCTGACAGTGGTACAGAGGCTGATGGAACGCAC
  AAAGCTGCCGTGGGATGCTGTGGGCCGTCTGGAAGTGGGCACCGAGACCATCATTGACAAGTCCAAGGCT
  GTCAAAACAGTGCTCATGGAACTGTTCCAGGATTCAGGCAACACTGACATCGAGGGCATAGATACCACCA
  ACGCCTGTTATGGCGGCACAGCCTCCCTCTTCAATGCTGCCAACTGGATGGAGTCCAGCTACTGGGATGG
  TCGCTATGCCCTGGTGGTCTGTGGTGACATTGCAGTCTACCCGAGTGGTAACGCCCGCCCCACAGGTGGT
  GCTGGGGCTGTGGCAATGCTGATCGGGCCCAAGGCCCCTCTGGTCCTCGAGCAAGGGCTGAGGGGAACTC
  ACATGGAGAACGCGTACGACTTCTACAAACCAAACTTGGCCTCAGAGTATCCACTGGTGGATGGGAAGCT
  GTCTATCCAGTGCTACCTGCGGGCCTTGGATCGATGCTATGCAGCCTACCGCAAGAAGATCCAGAATCAG
  TGGAAGCAAGCTGGAAACAACCAGCCTTTCACCCTCGATGATGTGCAGTATATGATCTTCCACACACCCT
  TTTGCAAGATGGTCCAGAAATCCCTGGCTCGGTTGATGTTCAATGACTTCCTGTCATCCAGCAGTGACAA
  ACAGAACAACTTATACAAGGGCCTAGAGGCCTTCAGGGGTCTAAAGCTGGAAGAAACCTACACCAACAAG
  GATGTAGACAAGGCTCTTTTGAAGGCCTCCCTGGACATGTTCAACCAGAAGACCAAGGCCTCCCTTTACC
  TCTCCACAAATAATGGGAACATGTACACCTCTTCCCTCTATGGCTGCCTGGCCTCACTTCTCTCTCACCA
  CTCTGCCCAAGAATTGGCTGGCTCCAGGATTGGAGCCTTCTCCTACGGCTCAGGCTTAGCAGCAAGTTTC
  TTTTCATTCCGAGTGTCCAAGGATGCTTCCCCAGGTTCCCCCCTGGAGAAACTGGTGTCTAGTGTGTCAG
  ATCTGCCCAAACGTCTAGACTCCCGGAGACGCATGTCCCCTGAGGAATTCACAGAAATAATGAACCAGAG
  AGAGCAATTTTACCACAAGGTGAACTTCTCTCCACCTGGAGACACAAGCAATCTCTTCCCAGGTACTTGG
  TACCTTGAACGAGTGGATGAGATGCATCGCAGGAAGTATGCCCGGTGTCCCGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208162 representing BC014714
  Red=Cloning site Green=Tags(s)
  
  MQRLLAPARRVLQVKRAMQETSLTPAHLLSAAQQRFSTIPPAPLAKTDTWPKDVGILALEVYFPAQYVDQ
  TDLEKFNNVEAGKYTVGLGQTRMGFCSVQEDINSLCLTVVQRLMERTKLPWDAVGRLEVGTETIIDKSKA
  VKTVLMELFQDSGNTDIEGIDTTNACYGGTASLFNAANWMESSYWDGRYALVVCGDIAVYPSGNARPTGG
  AGAVAMLIGPKAPLVLEQGLRGTHMENAYDFYKPNLASEYPLVDGKLSIQCYLRALDRCYAAYRKKIQNQ
  WKQAGNNQPFTLDDVQYMIFHTPFCKMVQKSLARLMFNDFLSSSSDKQNNLYKGLEAFRGLKLEETYTNK
  DVDKALLKASLDMFNQKTKASLYLSTNNGNMYTSSLYGCLASLLSHHSAQELAGSRIGAFSYGSGLAASF
  FSFRVSKDASPGSPLEKLVSSVSDLPKRLDSRRRMSPEEFTEIMNQREQFYHKVNFSPPGDTSNLFPGTW
  YLERVDEMHRRKYARCPV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC014714
• ORF Size: 1526 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC014714, AAH14714
• RefSeq Size: 2011
• RefSeq ORF: 1526
• Locus ID: 15360