Dld (NM_007861) Mouse Tagged ORF Clone

CAT#: MG208182

Dld (GFP-tagged) - Mouse dihydrolipoamide dehydrogenase (Dld)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dld
• Synonyms: AI315664; AI746344
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208182 representing NM_007861
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGCTGGAGTCGTGTGTACCGCTCCTTGGCCAAGAAAGGCCATTTCAATCGGATATCTCATGGCC
  TACAAGGAGTTTCTTCAGTTCCTTTAAGAACTTATGCAGATCAACCAATTGAGGCTGACGTGACAGTGAT
  AGGTTCTGGCCCTGGAGGATATGTTGCTGCCATCAAATCTGCCCAGTTAGGCTTTAAGACAGTCTGCATT
  GAGAAAAATGAGACACTAGGTGGGACATGCTTGAACGTTGGTTGTATTCCTTCAAAGGCTTTATTAAATA
  ATTCTCATTATTACCATATGGCCCACGGAAAAGATTTTGCATCTAGGGGAATTGAAATACCAGAAGTTCG
  CTTGAATTTAGAGAAGATGATGGAGCAGAAGCATTCTGCAGTAAAAGCATTAACAGGGGGAATTGCTCAC
  TTATTCAAACAAAATAAGGTTGTTCACGTCAATGGATTTGGAAAGATAACCGGCAAGAATCAAGTTACAG
  CTACAAAAGCCGATGGCAGCACTCAGGTTATTGATACCAAAAACATCCTTGTAGCTACGGGCTCAGAAGT
  TACTCCTTTTCCTGGAATCACGATCGATGAAGATACTATAGTGTCATCTACAGGAGCTTTATCCTTAAAA
  AAAGTTCCCGAGAAGCTGGTTGTTATTGGTGCAGGAGTAATTGGTGTGGAACTGGGTTCAGTTTGGCAAA
  GACTTGGTGCAGATGTGACAGCAGTTGAATTTTTGGGTCATGTGGGTGGAATTGGAATTGACATGGAGAT
  ATCAAAAAATTTTCAACGTATACTTCAGAGGCAAGGATTTAAGTTTAAACTGAATACAAAAGTTACTGGT
  GCCACCAAGAAGTCAGATGGAAAAATTGATGTGTCTGTCGAAGCTGCTTCTGGTGGTAAAGCTGAAGTTA
  TCACTTGTGATGTACTCTTGGTTTGCATTGGTCGGCGGCCCTTTACTCAGAATTTGGGACTAGAAGAGCT
  TGGAATTGAACTAGATCCCAAAGGTAGAATTCCAGTCAATAACAGATTTCAAACTAAAATTCCAAATATC
  TATGCTATTGGAGATGTGGTCGCTGGTCCAATGTTGGCTCACAAAGCAGAAGATGAAGGCATCATCTGTG
  TTGAAGGGATGGCAGGCGGTGCTGTGCACATTGATTATAATTGTGTGCCATCAGTGATTTACACACACCC
  CGAGGTTGCTTGGGTTGGCAAATCGGAAGAACAGTTGAAAGAAGAGGGTATTGAGTTCAAAATTGGAAAA
  TTCCCCTTTGCTGCAAACAGCAGAGCTAAAACAAATGCTGACACAGATGGCATGGTGAAGATTCTTGGAC
  ATAAGTCAACAGATAGAGTGCTGGGAGCACATATTCTAGGACCAGGTGCTGGAGAAATGGTGAACGAGGC
  GGCCCTGGCATTGGAATATGGTGCTTCCTGTGAAGACATAGCTCGAGTCTGCCATGCGCATCCGACCTTA
  TCAGAGGCTTTTAGAGAAGCAAACTTGGCTGCAGCTTTTGGAAAACCAATCAACTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208182 representing NM_007861
  Red=Cloning site Green=Tags(s)
  
  MQSWSRVYRSLAKKGHFNRISHGLQGVSSVPLRTYADQPIEADVTVIGSGPGGYVAAIKSAQLGFKTVCI
  EKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEIPEVRLNLEKMMEQKHSAVKALTGGIAH
  LFKQNKVVHVNGFGKITGKNQVTATKADGSTQVIDTKNILVATGSEVTPFPGITIDEDTIVSSTGALSLK
  KVPEKLVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGIGIDMEISKNFQRILQRQGFKFKLNTKVTG
  ATKKSDGKIDVSVEAASGGKAEVITCDVLLVCIGRRPFTQNLGLEELGIELDPKGRIPVNNRFQTKIPNI
  YAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEFKIGK
  FPFAANSRAKTNADTDGMVKILGHKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTL
  SEAFREANLAAAFGKPINF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007861
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007861.2, NP_031887.2
• RefSeq Size: 2220
• RefSeq ORF: 1530
• Locus ID: 13382
• Gene Summary: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. [provided by RefSeq, Jan 2014]