Nek3 (NM_011848) Mouse Tagged ORF Clone

CAT#: MG208192

Nek3 (GFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 3 (Nek3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nek3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208192 representing NM_011848
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAACTACACAGTCCTGAGAGTGATTGGCCAGGGCTCCTTCGGCAGGGCTCTTTTGGTTCTGCAGG
  AAAGCAGCAATCAGACATTTGCCATGAAGGAAATCAGACTGCTCAAGTCTGATACACAGACTTCTAGGAA
  AGAGGCTGTTCTGCTCGCCAAAATGAAGCACCCCAATATTGTTGCCTTTAAGGAGTCATTTGAAGCTGAA
  GGGTACCTGTACATTGTGATGGAGTATTGTGACGGAGGGGATCTGATGCAGAGGATTAAACAGCAAAAAG
  GGAAGTTATTCCCCGAAGACACGATACTTAATTGGTTTATACAAATTTGTCTTGGAGTAAATCACATTCA
  TAAGAGACGTGTTTTACACAGAGATATCAAGTCGAAAAATGTCTTCCTCACCCACAATGGAAAAGTGAAA
  TTGGGAGATTTTGGCTCTGCCCGTCTTCTCTCCAGTCCCATGGCATTTGCATGCACGTACGTGGGGACAC
  CTTATTATGTGCCTCCAGAAATCTGGGAGAACCTGCCTTACAACAATAAAAGTGACATCTGGTCCTTGGG
  ATGCATTCTGTATGAACTCTGCGCTCTGAAGCATCCATTCCAGGCAAATAGTTGGAAAAACCTGATCCTG
  AAAATATGCCAGGGGCCCATCCACCCGCTGCCGGCTCTATACTCCTGCAAGCTTCAGGACCTGGTCAAGC
  AGATGCTGAAAAGGAACCCTTCGCACCGCCCCTCCGTCACAACCCTGCTCTGCAGGGGTAGCTTGGCTCC
  ATTGGTCCTGAAGTGCTTGCCCCCTCAGATCATCAGAGAATATGGAGAACAGATATTAGATGAAATCAAA
  ATATCCACGCCTAAGAATATGAAAAAACAGGACTCCAACAGAGTCGGGAGAGCTCTGGGAGAAGCAAACT
  CTGCCTCTATGCAAGAGGAAGAACGTGGTAGAAAGTGTAGCCATACTGAATTGGAAAGCACTGGTACAAC
  CCCAGCTGGGAATGCATTGGGAAGAGCAGCCAGAGGAAATCCAGGTAACCCACAGGAGCACGGGAGGCAT
  ACCAGTCCGGCAAGTCCTCACAGGCCGCGGTGGGAGAGACACGGTCCCAGTTCCAATGTCGAAGCTTTGG
  AGAAAGCATCCATCCTTACCTCCAGTTTCACGGCAGAGGATGACAGAGGTGGGTCTGTGATAAAGTATGA
  AGAAAATGCCCGAAGGCAGTGGGTCAGAGAGCCCCCTGAGGCCTTGCTGTCAATGCTGAAGGACGCTGAT
  CTCAGTCAGGCATTTCAAACATACACAATATATAGACCAGGTGCAGAAGGATTCCTGAAAGGTCCCCTTT
  CTGAAGACACGGCATCGGACAGCGTCGACGGTGATCTCGATTCTGTCATGTTAGACCCAGAGAGATTTGA
  ACCTCGGCTGGATGAAGAAGACACGGACTTTGAGGAAGATAACGAAAACCCTGATTGGCTGTCAGAACTA
  AAGAAGCACGTAGGATACGGGGATGGACCTGGTGGACAACTCCTCGGAGAATGTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208192 representing NM_011848
  Red=Cloning site Green=Tags(s)
  
  MDNYTVLRVIGQGSFGRALLVLQESSNQTFAMKEIRLLKSDTQTSRKEAVLLAKMKHPNIVAFKESFEAE
  GYLYIVMEYCDGGDLMQRIKQQKGKLFPEDTILNWFIQICLGVNHIHKRRVLHRDIKSKNVFLTHNGKVK
  LGDFGSARLLSSPMAFACTYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCALKHPFQANSWKNLIL
  KICQGPIHPLPALYSCKLQDLVKQMLKRNPSHRPSVTTLLCRGSLAPLVLKCLPPQIIREYGEQILDEIK
  ISTPKNMKKQDSNRVGRALGEANSASMQEEERGRKCSHTELESTGTTPAGNALGRAARGNPGNPQEHGRH
  TSPASPHRPRWERHGPSSNVEALEKASILTSSFTAEDDRGGSVIKYEENARRQWVREPPEALLSMLKDAD
  LSQAFQTYTIYRPGAEGFLKGPLSEDTASDSVDGDLDSVMLDPERFEPRLDEEDTDFEEDNENPDWLSEL
  KKHVGYGDGPGGQLLGECA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011848
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011848.1, NP_035978.1
• RefSeq Size: 2025
• RefSeq ORF: 1530
• Locus ID: 23954