Hck (NM_010407) Mouse Tagged ORF Clone

CAT#: MG208418

Hck (GFP-tagged) - Mouse hemopoietic cell kinase (Hck)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hck
• Synonyms: AI849071; Bmk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208418 representing NM_010407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGGGGGTCGGTCTAGCTGCGAGGATCCGGGCTGCCCACGAAGCGAAGGGCGGGCGCCCAGGATGGGAT
  GCGTGAAGTCCAGGTTCCTCCGAGATGGAAGCAAGGCCTCAAAAACAGAGCCAAGTGCCAATCAGAAGGG
  CCCTGTGTATGTGCCGGATCCCACGTCCTCCAGCAAGCTGGGACCAAACAACAGCAACAGCATGCCCCCA
  GGGTTTGTGGAGGGCTCTGAGGATACCATTGTGGTCGCACTGTACGACTATGAGGCTATTCACCGTGAAG
  ACCTCAGCTTCCAGAAGGGAGACCAGATGGTGGTTCTGGAGGAGGCTGGGGAGTGGTGGAAGGCACGGTC
  CCTGGCTACCAAGAAGGAAGGCTACATCCCAAGCAACTATGTGGCTCGAGTTAACTCTTTGGAGACAGAA
  GAGTGGTTCTTCAAGGGGATCAGCCGGAAGGATGCAGAGCGCCACCTCCTGGCTCCAGGCAACATGCTGG
  GCTCCTTCATGATCCGGGACAGTGAGACCACCAAAGGGAGCTACTCGTTGTCTGTTCGAGACTTTGACCC
  CCAGCACGGAGACACCGTGAAGCACTATAAGATCCGGACGCTGGACAGTGGAGGCTTCTACATCTCTCCA
  AGGAGCACCTTCAGCAGCCTGCAGGAACTCGTGCTCCACTACAAGAAGGGGAAGGATGGGCTCTGCCAGA
  AGCTGTCAGTGCCCTGTGTGTCTCCCAAACCCCAGAAGCCATGGGAGAAAGATGCTTGGGAGATTCCTCG
  AGAATCCCTCCAGATGGAGAAGAAACTTGGAGCTGGGCAGTTTGGAGAAGTGTGGATGGCCACCTACAAC
  AAGCACACCAAAGTGGCGGTGAAGACAATGAAGCCAGGGAGCATGTCCGTGGAGGCCTTCCTGGCTGAGG
  CCAACCTGATGAAGTCGCTGCAGCATGACAAACTGGTGAAGCTACACGCTGTGGTCTCTCAGGAGCCCAT
  CTTTATTGTCACGGAGTTCATGGCCAAAGGAAGCCTGCTGGACTTTCTCAAGAGTGAAGAAGGCAGCAAG
  CAGCCACTGCCAAAACTCATTGACTTCTCAGCCCAGATCTCAGAAGGCATGGCCTTCATTGAGCAGAGGA
  ACTACATCCACCGAGACCTGAGGGCTGCCAACATCTTAGTCTCTGCATCACTGGTGTGTAAGATTGCTGA
  CTTTGGACTGGCACGAATCATCGAGGACAATGAGTACACAGCTCGGGAAGGAGCCAAGTTCCCCATCAAG
  TGGACAGCTCCTGAAGCCATCAACTTTGGTTCCTTCACCATCAAGTCAGATGTCTGGTCCTTTGGTATCC
  TGCTGATGGAAATTGTCACCTATGGCCGGATCCCTTACCCAGGTATGTCAAACCCAGAGGTGATTCGGGC
  ACTAGAGCATGGGTACCGTATGCCTCGACCAGATAACTGTCCAGAAGAGCTCTACAATATCATGATCCGC
  TGCTGGAAGAACCGCCCCGAGGAACGGCCCACCTTTGAATACATCCAGAGTGTGCTGGATGACTTCTACA
  CGGCCACTGAGAGCCAGTATCAGCAGCAGCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208418 representing NM_010407
  Red=Cloning site Green=Tags(s)
  
  LGGRSSCEDPGCPRSEGRAPRMGCVKSRFLRDGSKASKTEPSANQKGPVYVPDPTSSSKLGPNNSNSMPP
  GFVEGSEDTIVVALYDYEAIHREDLSFQKGDQMVVLEEAGEWWKARSLATKKEGYIPSNYVARVNSLETE
  EWFFKGISRKDAERHLLAPGNMLGSFMIRDSETTKGSYSLSVRDFDPQHGDTVKHYKIRTLDSGGFYISP
  RSTFSSLQELVLHYKKGKDGLCQKLSVPCVSPKPQKPWEKDAWEIPRESLQMEKKLGAGQFGEVWMATYN
  KHTKVAVKTMKPGSMSVEAFLAEANLMKSLQHDKLVKLHAVVSQEPIFIVTEFMAKGSLLDFLKSEEGSK
  QPLPKLIDFSAQISEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARIIEDNEYTAREGAKFPIK
  WTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALEHGYRMPRPDNCPEELYNIMIR
  CWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010407
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010407.3
• RefSeq Size: 2068
• RefSeq ORF: 1575
• Locus ID: 15162
• Gene Summary: The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq, Feb 2010]