Soat2 (NM_146064) Mouse Tagged ORF Clone

CAT#: MG208430

Soat2 (GFP-tagged) - Mouse sterol O-acyltransferase 2 (Soat2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Soat2
• Synonyms: ACAT2; D15Wsu97e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208430 representing NM_146064
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCAAAGGTGCCCCAGCTTCGGAGGAGAGAAGGGCTGGGAGAGGAGCAGGAGAAGGGAGCCCGTG
  GAGGAGAAGGGAACGCAAGGACACACGGAACCCCAGACTTGGTGCAATGGACTCGACATATGGAGGCTGT
  GAAGACACAGTTTCTGGAGCAAGCACAGAGAGAGTTGGCAGAGCTGTTGGATCGGGCCCTATGGGAGGCT
  ATGCAAGCTTACCCCAAACAAGACAGACCTCTTCCCTCCGCTGCCCCAGATTCTACAAGCAAGACCCAAG
  AGTTACACCCTGGAAAACGGAAAGTTTTCATCACCCGCAAGTCACTGATCGATGAGCTAATGGAGGTGCA
  ACATTTCCGAACCATCTACCACATGTTCATAGCGGGCCTATGCGTCCTGATCATCAGCACCCTGGCCATC
  GACTTCATTGATGAGGGCAGGTTGATGCTGGAGTTTGACTTACTCCTCTTCAGCTTCGGACAGCTGCCCT
  TGGCGCTGATGACCTGGGTTCCCATGTTCCTGTCTACGCTCCTAGTGCCCTACCAGACCCTGTGGCTGTG
  GGCCAGGCCGCGCGCTGGGGGTGCCTGGATGCTGGGGGCCAGCCTGGGCTGCGTTCTGCTGGCTGCCCAC
  GCTGTGGTGCTCTGCGTCCTGCCGGTGCACGTGTCAGTGAGGCATGAGCTTCCGCCCGCCTCGCGCTGCG
  TGCTGGTCTTTGAGCAGGTCAGATTGCTGATGAAAAGCTACTCCTTCCTGAGAGAGACTGTGCCTGGGAT
  CTTTTGTGTCAGAGGAGGAAAGGGCATCAGCCCCCCAAGTTTCTCCAGCTACCTCTACTTCCTCTTCTGC
  CCTACACTTATCTACAGAGAGACATACCCCAGGACACCCAGCATCAGGTGGAACTATGTGGCCAAGAACT
  TTGCCCAGGTCCTGGGCTGTTTGCTCTATGCCTGCTTCATCCTGGGCCGCCTCTGTGTCCCTGTCTTTGC
  CAACATGAGCCGGGAACCCTTCAGCACCCGGGCTCTGCTGCTCTCCATCTTGCATGCCACGGGGCCAGGC
  ATCTTCATGCTGCTCCTCATCTTCTTCGCCTTCCTGCACTGCTGGCTCAACGCCTTCGCCGAGATGCTGC
  GGTTTGGAGACAGGATGTTCTACCGGGACTGGTGGAACTCGACTTCCTTCTCCAACTACTACCGCACCTG
  GAACGTCGTGGTCCATGACTGGCTGTACAGCTATGTGTATCAAGATGGGCTGTGGCTCTTAGGCAGGCGG
  GCTCGCGGGGTGGCCATGCTGGGAGTGTTCCTGGTGTCTGCGGTGGTTCATGAGTATATCTTCTGCTTCG
  TCCTGGGGTTCTTCTACCCGGTCATGCTGATGCTATTCCTTGTTTTCGGGGGGCTGCTGAATTTCACCAT
  GAACGACAGGCACACAGGTCCAGCCTGGAACATCCTGATGTGGACCTTTCTCTTCATGGGCCAGGGCATC
  CAGGTCAGCCTATACTGCCAGGAGTGGTATGCTCGTCGACACTGTCCCCTGCCCCAGACAACATTCTGGG
  GGATGGTGACACCCCGCTCTTGGTCCTGCCATCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208430 representing NM_146064
  Red=Cloning site Green=Tags(s)
  
  MQPKVPQLRRREGLGEEQEKGARGGEGNARTHGTPDLVQWTRHMEAVKTQFLEQAQRELAELLDRALWEA
  MQAYPKQDRPLPSAAPDSTSKTQELHPGKRKVFITRKSLIDELMEVQHFRTIYHMFIAGLCVLIISTLAI
  DFIDEGRLMLEFDLLLFSFGQLPLALMTWVPMFLSTLLVPYQTLWLWARPRAGGAWMLGASLGCVLLAAH
  AVVLCVLPVHVSVRHELPPASRCVLVFEQVRLLMKSYSFLRETVPGIFCVRGGKGISPPSFSSYLYFLFC
  PTLIYRETYPRTPSIRWNYVAKNFAQVLGCLLYACFILGRLCVPVFANMSREPFSTRALLLSILHATGPG
  IFMLLLIFFAFLHCWLNAFAEMLRFGDRMFYRDWWNSTSFSNYYRTWNVVVHDWLYSYVYQDGLWLLGRR
  ARGVAMLGVFLVSAVVHEYIFCFVLGFFYPVMLMLFLVFGGLLNFTMNDRHTGPAWNILMWTFLFMGQGI
  QVSLYCQEWYARRHCPLPQTTFWGMVTPRSWSCHP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146064
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_146064.1, NP_666176.1
• RefSeq Size: 2126
• RefSeq ORF: 1578
• Locus ID: 223920
• Gene Summary: Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa. [UniProtKB/Swiss-Prot Function]