Pfkfb3 (BC052400) Mouse Tagged ORF Clone

CAT#: MG208437

Pfkfb3 (GFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (cDNA clone MGC:64634 IMAGE:5721049)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pfkfb3
• Synonyms: E330010H22, iPFK-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208437 representing BC052400
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTGGAACTGACCCAGAGCCGGGTACAGAAGATCTGGGTGCCCGTCGATCACCGACCCTCGTTGC
  CCAGATCCTGTGGGCCGAAGCTGACCAACTCCCCAACCGTGATTGTGATGGTGGGGCTCCCAGCCCGGGG
  TAAGACTTACATCTCCAAGAAGCTGACTCGCTACCTCAACTGGATAGGTGTTCCAACGAAAGTGTTCAAT
  GTGGGAGAGTATCGGCGTGAGGCTGTGAAGCAGTACAGCTCTTACAACTTCTTCCGCCCTGACAATGAGG
  AAGCCATGAGAGTCCGAAAACAGTGTGCCTTAGCTGCCCTGAGAGATGTCAAAAGCTACCTGACAAAGGA
  AGGTGGACAGATTGCAGTTTTTGATGCCACCAATACAACTAGAGAAAGAAGGCACATGATCCTTAATTTT
  GCCAAAGAAAATGACTTCAAGGCATTTTTCATCGAGTCGGTCTGTGACGACCCTACTGTTGTGGCCTCCA
  ACATCATGGAAGTTAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCAGCAGAAGCCATGGACGACTT
  CATGAAGAGAATCAATTGCTATGAAGCCAGCTACCAGCCTCTTGACCCTGATAAATGTGACAGGGACTTG
  TCACTCATCAAAGTGATTGATGTTGGCCGGAGGTTCTTGGTGAACAGAGTGCAGGACCACATCCAGAGCC
  GAATTGTATACTACCTGATGAACATCCACGTGCAGCCCCGTACCATCTACCTGTGTCGGCATGGCGAGAA
  TGAGTACAACTTGCAGGGCAAGATTGGGGGCGACTCAGGCTTGTCCAGCAGAGGCAAGAAGTTCGCCAAT
  GCCCTGAGCAAGTTCGTAGAAGAACAGAATCTGAAGGACCTCCGTGTGTGGACCAGCCAGCTGAAAAGTA
  CCATTCAGACCGCGGAGGCCCTGAGGCTCCCCTACGAGCAGTGGAAGGCACTCAACGAGATCGATGCTGG
  TGTGTGTGAGGAACTAACCTATGAGGAGATCAGAGATACCTACCCTGAGGAGTACGCACTGCGTGAACAG
  GACAAGTACTACTATCGCTATCCCACGGGAGAGTCCTACCAGGATCTTGTCCAACGCCTGGAGCCTGTGA
  TCATGGAATTAGAGCGCCAAGAGAATGTATTGGTCATCTGTCACCAGGCTGTTCTACGCTGCCTACTAGC
  CTACTTCCTCGACAAGAGTGCAGAGGAGATGCCATACCTGAAATGTCCGCTCCACACTGTCCTGAAACTG
  ACACCTGTGGCATATGGGTGCCGGGTGGAGTCCATTTACTTGAATGTAGAATCGGTGAGCACACACCGGG
  AGAGGTCAGAGGATGCAAAGAAGGGACCTAACCCGCTCATGAGACGCAATAGTGTCACCCCACTAGCCAG
  TCCCGAGCCCACCAAAAAGCCTCGCATCAACAGCTTTGAGGAGCGTGTGGCTTCCACTTCTGCTGCCCTG
  CCAAGCTGCCTGCCCCCGGAAGTGCCCACGCAGCTGCCCGGACAACCTTTGCTAGGGAAAGCCTGTCTAC
  GAAGTGTCTGTCACATTTTTTCAAAGTTTTCTCCTTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208437 representing BC052400
  Red=Cloning site Green=Tags(s)
  
  MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFN
  VGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILNF
  AKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDL
  SLIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNLQGKIGGDSGLSSRGKKFAN
  ALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQ
  DKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKL
  TPVAYGCRVESIYLNVESVSTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEERVASTSAAL
  PSCLPPEVPTQLPGQPLLGKACLRSVCHIFSKFSPY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052400
• ORF Size: 1580 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052400, AAH52400
• RefSeq Size: 4698
• RefSeq ORF: 1580
• Locus ID: 170768