Soat1 (NM_009230) Mouse Tagged ORF Clone

CAT#: MG208626

Soat1 (GFP-tagged) - Mouse sterol O-acyltransferase 1 (Soat1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Soat1
• Synonyms: 8430426K15Rik; Acact; ACAT-1; ald; AW550831; hid
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208626 representing NM_009230
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACTAAGAAACCGGCTGTCAAAATCTGGGGAGAATCCTGAGCAAGATGAAGCCCAGAAAAATTTCA
  TGGACACATACAGAAATGGTCACATTACCATGAAACAATTGATAGCCAAGAAGAGGCTGTTGGCAGCAGA
  GGCGGAGGAGCTGAAGCCACTATTTATGAAGGAAGTTGGGTGCCACTTCGATGACTTTGTGACCAACCTC
  ATTGAAAAGTCCGCATCGCTGGACAATGGTGGGTGTGCACTCACAACCTTCTCCATTCTTGAAGAAATGA
  AAAAGAACCACAGAGCCAAAGATCTGAGAGCACCTCCAGAACAAGGGAAGATTTTTATTTCAAGGCAGTC
  TCTTTTAGACGAGCTGTTTGAAGTGGACCACATCAGAACAATTTACCACATGTTCATCGCACTCCTCATC
  CTATTCGTCCTCAGCACGATCGTCGTGGACTACATCGATGAAGGAAGGCTGGTACTTGAGTTCAATCTCC
  TGGCCTATGCTTTTGGCAAATTTCCTACTGTTATTTGGACATGGTGGGCCATGTTCCTGTCTACACTGTC
  AATCCCCTATTTCCTGTTCCAGCGATGGGCCCACGGTTACAGCAAGAGTTCTCACCCATTGATCTATTCC
  CTTGTCCATGGCTTGCTCTTCTTAGTCTTTCAACTTGGAGTTCTAGGTTTTGTGCCAACATACGTTGTCT
  TAGCATACACACTGCCACCAGCCTCCCGGTTCATTCTGATACTGGAACAGATTCGTTTGATAATGAAGGC
  TCACTCATTTGTCAGAGAGAATATCCCACGAGTACTAAATGCAGCCAAGGAGAAATCAAGCAAAGATCCA
  CTACCCACAGTCAACCAGTACCTGTACTTCCTGTTTGCGCCTACACTTATTTACCGAGACAACTACCCAA
  GGACTCCTACTGTAAGATGGGGTTATGTCGCTATGCAGTTTTTACAGGTGTTTGGCTGCCTGTTTTATGT
  GTACTACATCTTTGAGAGACTCTGTGCCCCACTATTCCGGAATATCAAACAGGAGCCCTTCAGTGCTCGT
  GTCCTGGTCCTGTGTGTGTTTAACTCCATCTTGCCAGGTGTCTTGATACTGTTCCTTTCGTTCTTTGCCT
  TTTTGCACTGCTGGCTCAATGCCTTTGCTGAGATGTTACGCTTTGGTGACAGGATGTTTTATAAGGACTG
  GTGGAACTCTACATCATACTCCAACTACTACAGGACCTGGAACGTGGTGGTGCACGACTGGCTCTACTAC
  TATGTTTACAAAGACCTGCTCTGGTTTTTCTCGAAGAGGTTCAAATCTGCCGCCATGCTGGCCGTCTTCG
  CCCTGTCGGCTGTGGTGCACGAGTATGCCCTCGCCATCTGCCTGAGTTACTTCTACCCGGTGCTCTTCGT
  GCTCTTCATGTTCTTTGGAATGGCTTTTAACTTCATTGTTAACGACAGTCGGAAAAGGCCAATCTGGAAC
  ATCATGGTTTGGGCTTCTCTGTTCCTGGGCTATGGACTCATTCTGTGCTTTTATTCTCAAGAGTGGTATG
  CCCGCCAGCACTGTCCTCTGAAGAACCCTACATTTCTGGATTATGTCCGGCCGCGGACCTGGACTTGTCG
  GTACGTGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208626 representing NM_009230
  Red=Cloning site Green=Tags(s)
  
  MSLRNRLSKSGENPEQDEAQKNFMDTYRNGHITMKQLIAKKRLLAAEAEELKPLFMKEVGCHFDDFVTNL
  IEKSASLDNGGCALTTFSILEEMKKNHRAKDLRAPPEQGKIFISRQSLLDELFEVDHIRTIYHMFIALLI
  LFVLSTIVVDYIDEGRLVLEFNLLAYAFGKFPTVIWTWWAMFLSTLSIPYFLFQRWAHGYSKSSHPLIYS
  LVHGLLFLVFQLGVLGFVPTYVVLAYTLPPASRFILILEQIRLIMKAHSFVRENIPRVLNAAKEKSSKDP
  LPTVNQYLYFLFAPTLIYRDNYPRTPTVRWGYVAMQFLQVFGCLFYVYYIFERLCAPLFRNIKQEPFSAR
  VLVLCVFNSILPGVLILFLSFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYYRTWNVVVHDWLYY
  YVYKDLLWFFSKRFKSAAMLAVFALSAVVHEYALAICLSYFYPVLFVLFMFFGMAFNFIVNDSRKRPIWN
  IMVWASLFLGYGLILCFYSQEWYARQHCPLKNPTFLDYVRPRTWTCRYVF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009230
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009230.3, NP_033256.2
• RefSeq Size: 4785
• RefSeq ORF: 1623
• Locus ID: 20652
• Gene Summary: Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. [UniProtKB/Swiss-Prot Function]