Treh (BC019214) Mouse Tagged ORF Clone

CAT#: MG208643

Treh (GFP-tagged) - Mouse trehalase (brush-border membrane glycoprotein) (cDNA clone MGC:29262 IMAGE:5064708)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Treh
• Synonyms: 2210412M19Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208643 representing BC019214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTGGGAGCTGCACCTGCTGCTTCTGCTGGGGCTGGGACTTAGGTCCCAGGAGGCCCTGCCACCAC
  CCTGTGAGAGATCTACTGCCATGGAGAGCTCCTGCACCAAGTTCAGATGGCCCAGCTCTACCAAGATGAC
  AAGCAGTTTGTGGATATGTCACTGGCCACATCTCCAGATGAAGTCCTGCAGAAGTTCAGTGAGCTGGCCA
  CAGTCCACAACCACAGCATCCCCAAGGAACAGCTTCAGGAATTTGTCCAGAGTCACTTCCAGCCCGTGGG
  GCAGGAGCTGCAGTCCTGGACCCCTGAGGACTGGAAGGACAGCCCTCAGTTCCTGCAGAAGATCTCGGAT
  GCTAATCTGCGTGTCTGGGCGGAGGAGCTACACAAGATCTGGAAAAAGCTGGGAAAGAAGGGACTCGTAC
  TGGGTGATGGAAGGCCTGCTTCTTTCTGAGATGGCCTCAACAGTGAAGGGTATGCTGCAGAACTTTCTGG
  ATCTGGTGAAGACCTACGGACATATCCCCAATGGTGGACGCATATATTACCTGCAACGGAGCCAGCCCCC
  ACTCCTGACTCTCATGATGGATCGATATGTAGCTCATACCAAGGATGTCGCCTTCCTTCAGGAGAATATT
  GGGACTCTAGCCTCTGAACTGGACTTCTGGACTGTGAACAGGACTGTCTCTGTAGTCTCAGGAGGACAAA
  GCTATGTCTTAAATCGCTACTATGTCCCTTATGGGGGACCCAGGCCAGAGTCCTACAGGAAAGACGCAGA
  ATTGGCAAACTCTGTGCCAGAAGGGGACCGAGAGACTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCT
  GGCTGGGACTTCTCTTCACGCTGGCTTGTTGGAGGCCCAGACCCTGATTTGCTCAGCAGCATCCGAACCA
  GCAAAATGGTACCCGCTGATCTGAACGCGTTCCTGTGCCAAGCAGAGGAACTGATGAGTAACTTCTACTC
  CAGACTAGGGAACAACACAGAGGCCACAAAGTACAGGAACCTGCGGGCCCAGCGCTTGGCCGCCATGGAA
  GCTGTCCTGTGGGACGAGCAGAAGGGTGCCTGGTTCGACTATGACTTGGAAAAGGGGAAGAAGAACCTGG
  AGTTTTATCCCTCCAACCTCACCCCACTTTGGGCTGGCTGCTTCTCAGACCCTAGTGTTGCTGACAAGGC
  TCTGAAGTACTTGGAGGACAGCAAGATCTTGACCTACCAATATGGAATCCCAACCTCTCTTCGTAACACA
  GGCCAGCAGTGGGACTTCCCCAATGCCTGGGCCCCACTGCAGGACCTGGTCATTAGAGGTTTGGCCAAGT
  CAGCTTCCCCCCGGACTCAGGAGGTGGCTTTCCAGCTGGCCCAGAATTGGATCAAAACCAACTTCAAAGT
  CTACTCCCAAAAGTCAGCGATGTTTGAGAAGTATGACATCAGCAACGGTGGACATCCAGGTGGAGGAGGG
  GAGTATGAAGTTCAGGAAGGATTTGGCTGGACAAACGGATTGGCCCTGATGCTTCTGGATCGCTATGGTG
  ACCAGTTGACTTCAGGGACCCAGTTAGCTTCCCTGGGACCCCACTGCCTAGTGGCTGCCCTTCTTCTCAG
  TCTTCTGCTACAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208643 representing BC019214
  Red=Cloning site Green=Tags(s)
  
  MTWELHLLLLLGLGLRSQEALPPPCERSTAMESSCTKFRWPSSTKMTSSLWICHWPHLQMKSCRSSVSWP
  QSTTTASPRNSFRNLSRVTSSPWGRSCSPGPLRTGRTALSSCRRSRMLICVSGRRSYTRSGKSWERRDSY
  WVMEGLLLSEMASTVKGMLQNFLDLVKTYGHIPNGGRIYYLQRSQPPLLTLMMDRYVAHTKDVAFLQENI
  GTLASELDFWTVNRTVSVVSGGQSYVLNRYYVPYGGPRPESYRKDAELANSVPEGDRETLWAELKAGAES
  GWDFSSRWLVGGPDPDLLSSIRTSKMVPADLNAFLCQAEELMSNFYSRLGNNTEATKYRNLRAQRLAAME
  AVLWDEQKGAWFDYDLEKGKKNLEFYPSNLTPLWAGCFSDPSVADKALKYLEDSKILTYQYGIPTSLRNT
  GQQWDFPNAWAPLQDLVIRGLAKSASPRTQEVAFQLAQNWIKTNFKVYSQKSAMFEKYDISNGGHPGGGG
  EYEVQEGFGWTNGLALMLLDRYGDQLTSGTQLASLGPHCLVAALLLSLLLQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC019214
• ORF Size: 1623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC019214.1
• RefSeq Size: 1972
• RefSeq ORF: 1625
• Locus ID: 58866
• Gene Summary: This gene belongs to the alpha-glucosidase family, whose members encode enzymes that carry out hydrolysis of alpha-glucoside bonds of a variety of carbohydrates. The enzyme encoded by this gene uses the disaccharide trehalose as a highly specific substrate and converts it into two glucose molecules. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]