Gpc4 (BC006622) Mouse Tagged ORF Clone

CAT#: MG208834

Gpc4 (GFP-tagged) - Mouse glypican 4 (cDNA clone MGC:11506 IMAGE:3967797)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gpc4
• Synonyms: K-glypican
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208834 representing BC006622
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACGCTTAGGCTTGCTCGCGCTCCTCTGCACCCTGGCCGCGCTCAGCGCCTCGCTGCTGGCTGCGG
  AGCTCAAGTCGAAAAGTTGCTCGGAAGTGCGACGTCTCTACGTGTCCAAAGGCTTCAACAAGAATGATGC
  CCCCCTCTATGAGATCAACGGTGACCATTTGAAGATCTGTCCCCAGGATTATACATGCTGTTCTCAAGAG
  ATGGAAGAGAAGTACAGCCTGCAAAGTAAAGATGATTTCAAAACCGTGGTCAGCGAACAGTGCAACCATT
  TGCAAGCCATCTTTGCATCCCGTTACAAGAAGTTTGATGAATTCTTCAAAGAACTTCTTGAAAATGCAGA
  GAAATCCCTGAATGATATGTTCGTGAAGACATATGGCCACTTATACATGCAAAATTCAGAGCTATTTAAA
  GATCTCTTCGTTGAGTTGAAGCGCTACTATGTGGCGGGAAATGTGAACCTGGAAGAAATGTTAAATGACT
  TCTGGGCTCGCCTTCTGGAGCGCATGTTTCGCCTGGTGAACTCCCAGTACCATTTTACAGATGAGTACTT
  GGAATGTGTGAGCAAATATACAGAGCAGCTGAAGCCTTTTGGAGATGTCCCTCGGAAACTGAAGCTCCAG
  GTTACCCGCGCATTTGTTGCAGCCAGGACCTTCGCTCAAGGCTTAGCAGTTGCAAGGGATGTAGTAAGCA
  AAGTGTCCGTGGTGAACCCCACAGCTCAGTGCACCCATGCACTGCTGAAGATGATCTACTGCTCCCACTG
  CCGGGGCCTGGTGACTGTGAAGCCCTGTTACAACTATTGCTCAAACATCATGCGAGGCTGTTTGGCCAAC
  CAAGGAGACCTTGATTTTGAGTGGAACAATTTCATAGATGCTATGCTGATGGTGGCAGAGAGGCTGGAGG
  GTCCTTTCAACATTGAGTCCGTTATGGATCCCATCGATGTGAAGATTTCTGATGCTATCATGAATATGCA
  GGATAATAGTGTTCAAGTGTCTCAGAAGGTTTTCCAAGGCTGTGGCCCTCCGAAGCCTCTCCCAGCTGGA
  CGAATTTCTCGCTCCATCTCTGAAAGTGCCTTCAGTGCTCGATTCAGACCTTATCATCCAGAGCAACGCC
  CAACCACGGCAGCTGGCACTAGTTTGGACCGACTGGTTACTGATGTCAAGGAGAAACTGAAACAAGCTAA
  GAAGTTCTGGTCCTCTCTCCCAAGCACCGTTTGCAATGATGAGAGGATGGCAGCAGGAAATGAAAATGAG
  GATGACTGCTGGAATGGCAAAGGCAAAAGCAGGTACCTGTTTGCAGTGACAGGAAATGGATTGGCCAACC
  AGGGCAACAACCCAGAAGTCCAGGTTGACACCAGCAAGCCAGACATACTGATCCTTCGTCAGATCATGGC
  CCTTCGGGTTATGACCAGTAAAATGAAGAATGCTTACAATGGAAATGACGTGGACTTCTTTGACATCAGT
  GATGAGAGTAGTGGAGAGGGAAGTGGAAGCGGATGTGAATATCAGCAGTGCCCTTCGGAGTTCGAGTACA
  ACGCCACTGACCATTCTGGGAAGAGTGCCAACGAGAAAGCTGACAGTGCCGGTGGTGCCCATGCAGAGGC
  AAAGCCCTACCTCCTCGCTGCTCTCTGCATCCTGTTTCTCGCTGTGCAGGGAGAGTGGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208834 representing BC006622
  Red=Cloning site Green=Tags(s)
  
  MARLGLLALLCTLAALSASLLAAELKSKSCSEVRRLYVSKGFNKNDAPLYEINGDHLKICPQDYTCCSQE
  MEEKYSLQSKDDFKTVVSEQCNHLQAIFASRYKKFDEFFKELLENAEKSLNDMFVKTYGHLYMQNSELFK
  DLFVELKRYYVAGNVNLEEMLNDFWARLLERMFRLVNSQYHFTDEYLECVSKYTEQLKPFGDVPRKLKLQ
  VTRAFVAARTFAQGLAVARDVVSKVSVVNPTAQCTHALLKMIYCSHCRGLVTVKPCYNYCSNIMRGCLAN
  QGDLDFEWNNFIDAMLMVAERLEGPFNIESVMDPIDVKISDAIMNMQDNSVQVSQKVFQGCGPPKPLPAG
  RISRSISESAFSARFRPYHPEQRPTTAAGTSLDRLVTDVKEKLKQAKKFWSSLPSTVCNDERMAAGNENE
  DDCWNGKGKSRYLFAVTGNGLANQGNNPEVQVDTSKPDILILRQIMALRVMTSKMKNAYNGNDVDFFDIS
  DESSGEGSGSGCEYQQCPSEFEYNATDHSGKSANEKADSAGGAHAEAKPYLLAALCILFLAVQGEWR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC006622
• ORF Size: 1673 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC006622, AAH06622
• RefSeq Size: 2344
• RefSeq ORF: 1673
• Locus ID: 14735
• Gene Summary: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. [UniProtKB/Swiss-Prot Function]