Sptlc2 (NM_011479) Mouse Tagged ORF Clone

CAT#: MG208877

Sptlc2 (GFP-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 2 (Sptlc2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sptlc2
• Synonyms: LCB2; LCB2a; Spt2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208877 representing NM_011479
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCCGGAGCCCGGAGGCTGCTGCTGCCGCCGCCCGATGCGGGCGAACGGCTGCGTCAAGAACGGGG
  AAGTGAGGAACGGGTACTTGAGGAGCAGCACCGCCACCGTCGCGGCTGCCGGCCAGATTCATCATGTAAC
  AGAAAATGGAGGACTGTACAAAAGACCGTTTAATGAAGCTTTTGAAGAAACACCCATGCTGGTTGCTGTG
  CTCACATATGTGGGCTATGGCGTACTCACCCTCTTTGGATATCTTCGAGATTTCTTGAGGCATTGGAGAA
  TTGAAAAGTGCCACCATGCAACAGAAAGAGAAGAACAAAAGGACTTTGTGTCCTTGTATCAGGATTTTGA
  AAACTTCTATACAAGGAACCTCTACATGAGAATCAGAGACAACTGGAATCGACCTATCTGTAGTGTGCCT
  GGAGCCAAGGTGGATATCATGGAGAGAAAATCTCATGACTATAACTGGTCATTCAAGTACACAGGGAATA
  TAATTAAAGGTGTAATAAACATGGGTTCCTACAACTATCTTGGATTTGCGAGGAACACTGGATCATGTCA
  GGAAGCAGCTGCTGAAGTCCTCAAGGAGTATGGAGCAGGGGTGTGCAGCACTCGTCAGGAAATTGGAAAC
  CTGGACAAGCATGAAGAACTAGAGAAATTGGTAGCAAGGTTCTTAGGTGTGGAAGCTGCTATGACCTATG
  GCATGGGATTTGCAACAAATTCAATGAACATTCCTGCTCTTGTTGGCAAAGGTTGCCTGATTCTGAGTGA
  TGAGCTGAACCATGCGTCACTGGTTCTAGGAGCCAGACTGTCAGGAGCAACCATTCGAATCTTCAAACAC
  AACAATATGCAAAGCTTAGAGAAGCTTTTAAAAGATGCCATTGTTTATGGTCAGCCTCGGACAAGAAGAC
  CCTGGAAGAAAATCTTAATCCTTGTGGAAGGCATATATAGTATGGAGGGGTCTATTGTTCGCCTTCCTGA
  AGTGATTGCTCTCAAGAAGAAATACAAGGCATACTTGTATCTGGATGAGGCTCACAGCATTGGGGCTCTT
  GGCCCTTCAGGGCGAGGCGTGGTAGATTACTTTGGCCTGGATCCTGAGGATGTAGATGTTATGATGGGAA
  CATTCACAAAGAGCTTCGGTGCTTCAGGAGGATACATCGGAGGCAAGAAGGAGCTGATAGACTACCTGCG
  CACACATTCTCACAGTGCTGTGTATGCCACGTCGATGTCACCGCCTGTGATGGAACAGATTATCACCTCC
  ATGAAGTGCATCATGGGGCAGGATGGCACCAGTCTTGGCAAAGAATGTATACAGCAGTTGGCTGAGAACA
  CCAGGTATTTCAGGAGACGCCTGAAGGAAATGGGGTTCATCATCTATGGCAATGAAGACTCCCCGGTGGT
  GCCTTTGATGCTCTACATGCCGGCCAAAATTGGCGCCTTTGGAAGAGAGATGCTGAAGCGGAACATTGGT
  GTAGTTGTGGTGGGATTTCCTGCTACCCCGATCATTGAGTCCAGAGCCAGATTTTGCCTGTCAGCAGCTC
  ATACCAAAGAAATACTTGACACTGCTTTGAAGGAGATAGATGAAGTTGGGGATCTGCTGCAGCTAAAGTA
  CTCTCGCCACCGGCTGGTGCCTCTACTGGACAGGCCCTTTGATGAGACTACCTATGAAGAGACAGAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208877 representing NM_011479
  Red=Cloning site Green=Tags(s)
  
  MRPEPGGCCCRRPMRANGCVKNGEVRNGYLRSSTATVAAAGQIHHVTENGGLYKRPFNEAFEETPMLVAV
  LTYVGYGVLTLFGYLRDFLRHWRIEKCHHATEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVP
  GAKVDIMERKSHDYNWSFKYTGNIIKGVINMGSYNYLGFARNTGSCQEAAAEVLKEYGAGVCSTRQEIGN
  LDKHEELEKLVARFLGVEAAMTYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIRIFKH
  NNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGAL
  GPSGRGVVDYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSMSPPVMEQIITS
  MKCIMGQDGTSLGKECIQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIG
  VVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011479
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011479.2, NP_035609.1
• RefSeq Size: 3754
• RefSeq ORF: 1683
• Locus ID: 20773
• Gene Summary: This gene encodes a long chain base subunit of serine palmitoyltransferase. The enzyme, serine palmitoyltransferase, consists of two different subunits, and is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. A mutant allele of this gene in mice is used as a model for the human disease 'Susceptibilty to Psoriasis 1'. Mutations in the human gene are associated with hereditary sensory neuropathy type I. [provided by RefSeq, Sep 2015]