Sgpl1 (NM_009163) Mouse Tagged ORF Clone

CAT#: MG208957

Sgpl1 (GFP-tagged) - Mouse sphingosine phosphate lyase 1 (Sgpl1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sgpl1
• Synonyms: AI428538; D10Xrf456; S1PL; Spl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208957 representing NM_009163
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGAACCGACCTCCTCAAGCTGAAGGACTTCGAGCCTTATTTGGAGATTTTGGAATCATATTCCA
  CAAAAGCCAAGAATTATGTGAATGGATATTGCACCAAATATGAGCCCTGGCAGCTCATTGCGTGGAGTGT
  CCTGTGTACTCTGCTGATAGTCTGGGTGTATGAGCTTATCTTCCAGCCAGAGAGTTTATGGTCTCGGTTT
  AAAAAAAAATTATTTAAGCTTATCAGGAAGATGCCATTTATTGGACGTAAGATCGAACAACAGGTGAGCA
  AAGCCAAGAAGGATCTTGTCAAGAACATGCCATTCCTAAAGGTGGACAAGGATTATGTGAAAACTCTGCC
  TGCTCAGGGTATGGGCACAGCTGAGGTTCTGGAGAGACTCAAGGAGTACAGCTCCATGGATGGTTCCTGG
  CAAGAAGGGAAAGCCTCAGGAGCTGTGTACAATGGGGAACCGAAGCTCACGGAGCTGCTGGTGCAGGCTT
  ATGGAGAATTCACGTGGAGCAATCCACTGCATCCAGATATCTTCCCTGGATTGCGGAAGTTAGAGGCAGA
  AATCGTTAGGATGACTTGTTCCCTCTTCAATGGGGGACCAGATTCCTGTGGATGTGTGACTTCTGGGGGA
  ACGGAAAGCATCCTGATGGCCTGCAAAGCTTACCGGGACTTGGCGTTAGAGAAGGGGATCAAAACTCCAG
  AAATTGTGGCTCCCGAGAGTGCCCATGCTGCATTCGACAAAGCAGCTCATTATTTTGGGATGAAGATTGT
  CCGAGTTGCACTGAAAAAGAACATGGAGGTGGATGTGCAGGCAATGAAGAGAGCCATCTCCAGGAACACA
  GCTATGCTGGTCTGTTCTACCCCACAGTTTCCTCATGGTGTGATGGATCCTGTCCCCGAAGTGGCCAAGT
  TGGCTGTCAGATATAAAATCCCACTCCATGTGGATGCTTGTCTGGGGGGCTTCCTCATTGTCTTCATGGA
  GAAAGCAGGGTACCCACTGGAGAAACCATTTGATTTCCGGGTGAAAGGTGTGACCAGCATTTCAGCAGAT
  ACTCATAAGTATGGCTATGCTCCTAAAGGTTCATCAGTGGTGATGTACTCTAACGAGAAGTACAGGACGT
  ACCAGTTCTTTGTTGGTGCAGACTGGCAAGGTGGTGTCTACGCATCTCCAAGCATAGCTGGCTCACGGCC
  TGGTGGCATCATTGCAGCCTGTTGGGCCGCCTTGATGCACTTCGGTGAGAACGGCTATGTTGAAGCTACC
  AAACAGATCATCAAAACTGCTCGCTTCCTGAAGTCAGAACTGGAAAACATCAAAAACATCTTCATTTTCG
  GTGATCCTCAATTGTCAGTTATTGCTCTGGGATCCAACGATTTTGACATTTACCGACTATCTAATATGAT
  GTCTGCTAAGGGGTGGAATTTTAACTACCTGCAGTTCCCAAGAAGCATTCATTTCTGCATTACGTTAGTA
  CATACTCGGAAGCGAGTGGCGATCCAGTTCCTAAAGGATATCCGGGAATCAGTCACACAAATCATGAAGA
  ATCCTAAAGCTAAGACCACAGGAATGGGTGCCATCTATGGCATGGCCCAGGCAACCATTGACAGGAAGCT
  GGTTGCAGAAATATCCTCCGTCTTCTTGGACTGCCTTTATACTACGGACCCCGTGACTCAGGGCAACCAG
  ATGAACGGTTCTCCAAAGCCCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208957 representing NM_009163
  Red=Cloning site Green=Tags(s)
  
  MPGTDLLKLKDFEPYLEILESYSTKAKNYVNGYCTKYEPWQLIAWSVLCTLLIVWVYELIFQPESLWSRF
  KKKLFKLIRKMPFIGRKIEQQVSKAKKDLVKNMPFLKVDKDYVKTLPAQGMGTAEVLERLKEYSSMDGSW
  QEGKASGAVYNGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGG
  TESILMACKAYRDLALEKGIKTPEIVAPESAHAAFDKAAHYFGMKIVRVALKKNMEVDVQAMKRAISRNT
  AMLVCSTPQFPHGVMDPVPEVAKLAVRYKIPLHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISAD
  THKYGYAPKGSSVVMYSNEKYRTYQFFVGADWQGGVYASPSIAGSRPGGIIAACWAALMHFGENGYVEAT
  KQIIKTARFLKSELENIKNIFIFGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLV
  HTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKLVAEISSVFLDCLYTTDPVTQGNQ
  MNGSPKPR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009163
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009163.2, NP_033189.2
• RefSeq Size: 4108
• RefSeq ORF: 1707
• Locus ID: 20397