Nfib (BC014290) Mouse Tagged ORF Clone

CAT#: MG208973

Nfib (GFP-tagged) - Mouse nuclear factor I/B (cDNA clone MGC:13959 IMAGE:4038233)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nfib
• Synonyms: 6720429L07Rik|CTF|E030026I10Rik|NF-I; B|NF1-B|NFI-B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208973 representing BC014290
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTATTCTCCCATCTGTCTCACTCAGGATGAATTCCACCCATTTATTGAGGCACTTCTTCCTCACG
  TCCGTGCAATTGCCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAGTACTTTAAAAAGCATGAGAA
  ACGAATGTCGAAGGATGAAGAAAGGGCAGTCAAAGACGAGCTGCTCAGTGAGAAGCCCGAAATCAAGCAG
  AAGTGGGCATCCAGGCTCCTGGCCAAACTGCGCAAAGATATCCGCCAGGAGTACCGGGAGGACTTTGTGC
  TTACCGTGACTGGCAAGAAGCACCCGTGCTGTGTCTTATCCAATCCAGACCAGAAGGGTAAGATTAGGAG
  GATCGACTGCCTGCGACAGGCAGACAAAGTCTGGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGC
  ATCCCTTTGGAGAGTACGGATGGAGAGCGACTCATGAAGTCCCCGCACTGCACAAACCCAGCACTTTGTG
  TTCAGCCACACCACATCACAGTATCAGTTAAGGAGCTTGACTTGTTTTTGGCATACTACGTGCAGGAGCA
  AGATTCTGGACAATCAGGAAGTCCAAGCCACAGTGATCCTGCCAAGAATCCTCCAGGGTACCTCGAGGAC
  AGCTTTGTAAAATCCGGAGTCTTCAATGTATCAGAGCTTGTGAGAGTATCCAGAACACCCATAACCCAGG
  GAACTGGAGTCAACTTCCCAATCGGAGAAATTCCCAGCCAACCATACTATCATGACATGAACTCTGGTGT
  GAACCTGCAGAGGTCGCTGTCTTCTCCACCGAGCAGCAAAAGACCCAAAACTATATCTATAGATGAAAAT
  ATGGAGCCAAGTCCTACAGGAGACTTTTACCCCTCTCCAAATTCACCAGCTGCTGGAAGTCGAACATGGC
  ATGAACGAGATCAAGATATGTCTTCTCCAACTACAATGAAGAAGCCTGAGAAGCCACTGTTTAGCTCTAC
  ATCTCCACAGGATTCTTCCCCAAGATTGAGCACTTTCCCCCAGCACCATCATCCCGGAATACCTGGAGTC
  GCGCACAGTGTCATCTCAACTCGAACTCCACCTCCGCCCTCACCGTTGCCATTTCCGACGCAAGCTATCC
  TTCCTCCGGCACCTTCCAGCTACTTCTCTCATCCAACAATCAGATATCCTCCTCACCTGAATCCTCAGGA
  TACTCTGAAGAACTACGTACCTTCTTATGACCCATCCAGTCCTCAAACGAGCCAGCCTAACAGCAGTGGT
  CAAGTAGTAGGGAAAGTGCCTGGCCATTTCACACCTGTCTTGGCACCCTCTCCCCATCCCAGTGCAGTGC
  GACCTGTGACCCTGACCATGACAGATACTAAACCCATCACTACATCCACTGAAGGTGAGGCAGCTTCACC
  TACAGCAACCACCTACACAGCCTCAGGCACATCTCAAGCCAATCGATATGTGGGACTAAGCCCAAGAGAC
  CCATCCTTCCTGCATCAGCAACAGCTGAGGATTTGTGACTGGACCATGAATCAAAACGGCAGGCATTTAT
  ACCCCAGTACCAGTGAGGATACATTGGGAATTACTTGGCAAAGTCCTGGTACCTGGGCTAGCTTGGTTCC
  TTTTCAAGTGTCAAATAGGACACCCATCTTACCGGCAAATGTCCAAAATTATGGTTTGAACATAATTGGA
  GAGCCTTTCCTTCAAGCGGAGACAAGCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208973 representing BC014290
  Red=Cloning site Green=Tags(s)
  
  MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQ
  KWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKG
  IPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFLAYYVQEQDSGQSGSPSHSDPAKNPPGYLED
  SFVKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDEN
  MEPSPTGDFYPSPNSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSTSPQDSSPRLSTFPQHHHPGIPGV
  AHSVISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQPNSSG
  QVVGKVPGHFTPVLAPSPHPSAVRPVTLTMTDTKPITTSTEGEAASPTATTYTASGTSQANRYVGLSPRD
  PSFLHQQQLRICDWTMNQNGRHLYPSTSEDTLGITWQSPGTWASLVPFQVSNRTPILPANVQNYGLNIIG
  EPFLQAETSN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC014290
• ORF Size: 1710 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC014290, AAH14290
• RefSeq Size: 2386
• RefSeq ORF: 1712
• Locus ID: 18028
• Gene Summary: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. [UniProtKB/Swiss-Prot Function]