Sqle (NM_009270) Mouse Tagged ORF Clone

CAT#: MG209004

Sqle (GFP-tagged) - Mouse squalene epoxidase (Sqle)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sqle
• Synonyms: AI323792
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209004 representing NM_009270
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACTTTTCTTGGCATTGCTACCTTCACCTATTTTTATAAGAAATGCGGGGATGTCACCCTGGCCA
  ACAAGGAGCTCCTGCTGTGCGTGCTGGTGTTCCTGTCGCTGGGCCTGGTGCTCTCCTACCGCTGTCGCCA
  TCGACACGGGGGCCTCCTTGGCCGCCATCAGAGCGGCGCCCAGTTCGCTGCCTTCTCGGATATTCTCTCT
  GCTTTGCCTCTTATTGGCTTCTTCTGGGCCAAGTCACCTGAATCAGAAAAGAAAGAACAGCTGGAGTCCA
  AGAAGTGCAGAAAAGAAATCGGTCTTTCAGAAACGACATTAACAGGAGCAGCTACCTCAGTGTCGACCTC
  GTTCGTGACGGACCCGGAAGTGATCATCGTGGGATCTGGTGTGCTTGGATCTGCCTTGGCAGCAGTTCTC
  TCCAGAGATGGAAGAAAGGTGACGGTCATCGAGAGAGATTTAAAAGAGCCCGACAGGATAGTTGGGGAGC
  TGCTGCAGCCAGGAGGCTACCGTGTTCTCCAGGAGCTGGGCCTTGGAGATACAGTAGAAGGTCTCAATGC
  CCATCATATACATGGCTACATAGTTCATGACTATGAAAGCAGATCTGAAGTTCAAATTCCGTACCCACTG
  TCAGAAACCAACCAAGTGCAGAGTGGAATTGCTTTTCACCATGGCCGATTCATCATGAGTCTCCGGAAAG
  CAGCTATGGCAGAGCCCAATGTAAAGTTTATAGAAGGTGTTGTGCTTCAGTTACTAGAGGAAGATGATGC
  TGTAATCGGCGTGCAATACAAGGACAAGGAGACTGGGGACACCAAGGAGCTCCACGCCCCGCTCACCGTT
  GTTGCGGATGGACTCTTCTCCAAGTTCAGGAAGAGCCTCATCTCCAGTAAAGTCTCCGTTTCTTCCCACT
  TCGTTGGCTTCCTTATGAAGGATGCACCACAGTTTAAACCCAATTTTGCGGAGCTTGTTCTGGTCAACCC
  CAGTCCAGTTCTCATCTACCAGATTTCTTCCAGTGAAACTAGGGTACTTGTTGATATTCGGGGAGAATTG
  CCAAGAAACCTAAGAGAATACATGGCTGAACAAATTTACCCACAGTTACCTGAGCACCTGAAGGAGTCAT
  TTCTGGAGGCCTCTCAGAATGGTCGTCTGCGGACCATGCCGGCCAGCTTCCTTCCTCCTTCCTCAGTGAA
  CAAACGAGGCGTCCTTATTCTGGGAGATGCGTATAACCTGAGGCACCCTCTTACTGGTGGAGGAATGACA
  GTTGCTTTAAAAGATATAAAATTGTGGAGACAACTGTTAAAAGACATTCCTGACCTTTATGATGATGCTG
  CTATTTTCCAGGCCAAAAAATCATTCTTTTGGTCAAGAAAAAGGACCCATTCCTTTGTTGTGAATGTATT
  GGCTCAGGCCTTGTATGAATTATTTTCAGCTACAGATGATTCCTTGCATCAGCTCCGAAAAGCTTGCTTT
  CTGTATTTTAAACTTGGTGGAGAGTGTGTGACCGGTCCTGTTGGGTTGCTTTCAATATTGTCTCCTCACC
  CTCTGGTGTTGATTCGACACTTCTTTTCCGTTGCAATCTACGCCACGTATTTCTGCTTCAAATCAGAGCC
  GTGGGCGACAAAACCACGGGCCCTTTTCAGTAGCGGTGCTGTACTGTACAAAGCGTGTTCTATCCTATTT
  CCACTCATCTATTCAGAAATGAAGTATCTGGTTCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209004 representing NM_009270
  Red=Cloning site Green=Tags(s)
  
  MWTFLGIATFTYFYKKCGDVTLANKELLLCVLVFLSLGLVLSYRCRHRHGGLLGRHQSGAQFAAFSDILS
  ALPLIGFFWAKSPESEKKEQLESKKCRKEIGLSETTLTGAATSVSTSFVTDPEVIIVGSGVLGSALAAVL
  SRDGRKVTVIERDLKEPDRIVGELLQPGGYRVLQELGLGDTVEGLNAHHIHGYIVHDYESRSEVQIPYPL
  SETNQVQSGIAFHHGRFIMSLRKAAMAEPNVKFIEGVVLQLLEEDDAVIGVQYKDKETGDTKELHAPLTV
  VADGLFSKFRKSLISSKVSVSSHFVGFLMKDAPQFKPNFAELVLVNPSPVLIYQISSSETRVLVDIRGEL
  PRNLREYMAEQIYPQLPEHLKESFLEASQNGRLRTMPASFLPPSSVNKRGVLILGDAYNLRHPLTGGGMT
  VALKDIKLWRQLLKDIPDLYDDAAIFQAKKSFFWSRKRTHSFVVNVLAQALYELFSATDDSLHQLRKACF
  LYFKLGGECVTGPVGLLSILSPHPLVLIRHFFSVAIYATYFCFKSEPWATKPRALFSSGAVLYKACSILF
  PLIYSEMKYLVH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009270
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009270.3, NP_033296.1
• RefSeq Size: 2748
• RefSeq ORF: 1719
• Locus ID: 20775
• Gene Summary: Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway. [UniProtKB/Swiss-Prot Function]