Me2 (NM_145494) Mouse Tagged ORF Clone

CAT#: MG209185

Me2 (GFP-tagged) - Mouse malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Me2
• Synonyms: AW120568; D030040L20Rik; NAD-ME
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209185 representing NM_145494
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTCCCGGCTAAGAGCTGTTACCACTCCTTGTACCTTGACCTGCCGCCGAGTGCACCTGAAAGAGA
  AGGGCAAGCCACTCATGTTGAATCCAAGAACAAACAAGGGAATGGCGTTTACGTTACAAGAGCGACAAAT
  GCTTGGTCTTCAAGGACTTTTACCTCCTAAAATAGAGACACAGGATATTCAAGCCTTACGATTCCATAGA
  AACTTGAAAAAAATGACTAGCCCTTTGGAAAAATACATTTATATAATGGGCATCCAAGAAAGAAATGAGA
  AACTGTTCTATAGAATCCTGCAGGATGATATTGAAAGTCTGATGCCGATTGTGTACACGCCAACAGTTGG
  TCTTGCCTGCTGTCAATATGGGCACATCTTTAGGAGACCTAAGGGATTGTTTATTTCAATCTCAGACAGA
  GGTCATGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTGGTGACTGATGGAG
  AGAGGATCCTGGGCCTTGGCGATCTGGGTGTCTATGGAATGGGGATTCCAGTAGGGAAGCTGTGCCTGTA
  TACAGCCTGTGCAGGGATACAGCCCGAGAAGTGCCTGCCCGTGTGCATCGACGTGGGCACTGATAACATG
  GCACTATTAAAAGATCCGTTTTATATGGGCTTGTATCAGAAGCGAGATCGTTCCCAGCTCTATGATGATC
  TGATGGACGAGTTTATGAAGGCGATTACTGACAGATACGGCCGGAACACACTCATTCAGTTTGAAGACTT
  TGGAAATCATAATGCATTCAGGTTCTTAAGAAAATATCAACAAAAATACTGTACCTTCAATGATGACATT
  CAAGGGACAGCTGCAGTTGCTCTGTCTGGTCTTCTTGCGGCCCAAAGAGTTATTAATAAACCAGTCTCAG
  AACACAAAATCTTATTTCTTGGAGCAGGAGAAGCTGCACTTGGAATTGCCAATCTCATAGTTCTATCAAT
  GGTGGAAAGTGGTCTCTCAGAAGAGGAGGCGCAGAGAAAGATCTGGATGTTTGACAAGAGTGGCTTGCTG
  GTTAAGGGGCGGACTGCTAGCATAGATAGCAACCAGGAACCATATGCCCATGCGGCCCCAGAGAGCATAC
  CTGCGACTTTTGAAGATGCAGTAAATAAACTTAAGCCTTCAGTTATAATTGGAGTGGCAGGTGCCGGCCC
  GCTTTTTACTCACGGTGTAATCAAAGCCATGGCCTCTATCAACGAGAGACCTATAATATTTGCTCTAAGT
  AATCCTACAGCACAGGCCGAGTGCACAGCCGAGGACGCGTACACGCTCACAGAGGGCAGGTGTCTGTTTG
  CCAGTGGCAGCCCATTTGAGCCAGTGAAACTCCAGGATGGACGAGTCTTCACCCCAGGCCAAGGAAACAA
  TGCATATATTTTCCCAGGTGTGGCTTTAGCTGTTATTCTCTGTGAAGCCCGACACATCAGTGACACCGTT
  TTCTTAGAAGCTGCAAAGGCGCTGACGACTCAGTTGACAGATGCAGAGTTAGCCCAAGGGAGGCTTTACC
  CGTCGCTGGCTAATATTCAGGAAGTTTCTGCTAACATAGCTATTAAACTTGCAGAGTACCTGTACGCTAA
  TAAAATGGCCTTCCGGTACCCAGAACCCGAGGACAAGGCCAGATACGTGAGAGAGAGAATATGGCGGAGC
  AACTACGTTTCCCTGCTGCCGGACGTGTATGACTGGCCAGAGTCTTCACTGACGCCTCCTCAGATCACAG
  AAGAGAAGCTTCCCCAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209185 representing NM_145494
  Red=Cloning site Green=Tags(s)
  
  MFSRLRAVTTPCTLTCRRVHLKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHR
  NLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDR
  GHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNM
  ALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYQQKYCTFNDDI
  QGTAAVALSGLLAAQRVINKPVSEHKILFLGAGEAALGIANLIVLSMVESGLSEEEAQRKIWMFDKSGLL
  VKGRTASIDSNQEPYAHAAPESIPATFEDAVNKLKPSVIIGVAGAGPLFTHGVIKAMASINERPIIFALS
  NPTAQAECTAEDAYTLTEGRCLFASGSPFEPVKLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTV
  FLEAAKALTTQLTDAELAQGRLYPSLANIQEVSANIAIKLAEYLYANKMAFRYPEPEDKARYVRERIWRS
  NYVSLLPDVYDWPESSLTPPQITEEKLPH
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145494
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145494.2, NP_663469.1
• RefSeq Size: 2455
• RefSeq ORF: 1770
• Locus ID: 107029