Numb (BC033459) Mouse Tagged ORF Clone

CAT#: MG209237

Numb (GFP-tagged) - Mouse numb gene homolog (Drosophila) (cDNA clone MGC:28234 IMAGE:3991630)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Numb
• Synonyms: mnb, m-numb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209237 representing BC033459
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAACTACGGCAAAGCTTCAGGAGAAAGAAAGACGTTTATGTCCCAGAGGCCAGCCGTCCACATC
  AGTGGCAGACAGATGAAGAAGGAGTCCGCACTGGAAAGTGTAGCTTCCCAGTTAAGTACCTCGGCCACGT
  AGAAGTTGATGAGTCAAGAGGAATGCACATCTGTGAAGATGCCGTAAAGAGATTGAAAGCTACGGGAAAG
  AAAGCAGTGAAGGCCGTTCTGTGGGTGTCAGCGGATGGGCTCAGAGTTGTGGACGAGAAAACTAAGGACC
  TCATAGTTGACCAGACAATAGAAAAAGTTTCCTTCTGTGCCCCAGATAGGAACTTTGACAGAGCCTTTTC
  TTACATATGTCGCGATGGCACCACTCGGCGATGGATCTGTCATTGTTTCATGGCTGTCAAAGACACGGGG
  GAAAGACTGAGCCACGCCGTGGGCTGTGCTTTTGCAGCCTGTTTAGAGCGTAAACAGAAGCGGGAGAAGG
  AGTGTGGCGTCACTGCTACTTTTGATGCCAGTAGAACCACTTTCACAAGAGAAGGATCATTCCGTGTCAC
  AACTGCCACTGAGCAAGCCGAAAGAGAGGAGATCATGAAACAGTTGCAAGATGCCAAGAAAGCTGAGACA
  GACAAGACAGCTGTTGGTCCATCAGTGGCTCCTGGCAACACTGCTCCATCCCCATCCTCTCCCACCTCTC
  CCACTCCGGATGGCACTGCATCTTCAGAGATGAACAATCCCCATGCTATCCCACGCCGGCATGCACCAAT
  TGAACAGCTTGCTCGTCAAGGCTCTTTCCGGGGATTTCCTGCTCTTAGCCAGAAGATGTCACCCTTTAAA
  CGCCAGCTGTCCCTCCGCATCAATGAGTTGCCTTCCACTATGCAGAGGAAGACTGATTTCCCAATAAAAA
  ACACAGTGCCCGAGGTGGAAGGAGAGGCCGAGAGCATCAGCTCCTTGTGTTCCCAGATCACCAGTGCCTT
  CAGCACGCCCTCTGAGGACCCCTTCTCCTCCGCCCCAATGACCAAACCAGTGACATTGGTGGCACCACAG
  TCTCCTGTGTTACAAGGGACTGAGTGGGGTCAGTCTTCTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCTG
  GTCACAGACGCACTCCCTCTGAAGCTGACCGCTGGTTAGAAGAAGTGTCAAAGAGTGTGCGGGCCCAGCA
  GCCTCAGGTCTCAGCTGCCCCTCTGCAGCCAGTTCTCCAGCCGCCTCCGCCCGCCGCCATTGCCCCTCCA
  GCACCTCCTTTCCAAGGACATGCCTTCCTCACGTCCCAGCCTGTGCCCGTGGGTGTGGTCCCACCCCTAC
  AACCAGCCTTTGTCCCTACCCAGTCCTACCCTGTGGCCAACGGGATGCCCTACCCAGCCTCTAATGTGCC
  TGTAGTGGGCATCACCCCATCCCAGATGGTAGCCAATGTGTTTGGCACTGCAGGCCACCCTCAGACCACT
  CATCCACATCAGTCGCCAAGCCTGGCCAAGCAGCAGACATTCCCTCAATATGAGACAAGTAGTGCTACCA
  CCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAATGGTTCTGCAGCTTTCAATGGTGTAGACAATGG
  TGGGCTAGCCTCAGGAAACAGGCATGCAGAAGTCCCTCCAGGCACCTGCCCAGTGGATCCTTTCGAAGCT
  CAGTGGGCCGCACTAGAAAGCAAGTCCAAGCAGCGTACAAATCCTTCTCCTACCAACCCTTTCTCCAGTG
  ACTTACAGAAAACGTTTGAAATAGAACTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209237 representing BC033459
  Red=Cloning site Green=Tags(s)
  
  MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKATGK
  KAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTG
  ERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQLQDAKKAET
  DKTAVGPSVAPGNTAPSPSSPTSPTPDGTASSEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFK
  RQLSLRINELPSTMQRKTDFPIKNTVPEVEGEAESISSLCSQITSAFSTPSEDPFSSAPMTKPVTLVAPQ
  SPVLQGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQVSAAPLQPVLQPPPPAAIAPP
  APPFQGHAFLTSQPVPVGVVPPLQPAFVPTQSYPVANGMPYPASNVPVVGITPSQMVANVFGTAGHPQTT
  HPHQSPSLAKQQTFPQYETSSATTSPFFKPPAQHLNGSAAFNGVDNGGLASGNRHAEVPPGTCPVDPFEA
  QWAALESKSKQRTNPSPTNPFSSDLQKTFEIEL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC033459
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC033459.1
• RefSeq Size: 3495
• RefSeq ORF: 1781
• Locus ID: 18222
• Gene Summary: This gene encodes a conserved protein that is distributed asymmetrically during cell division in the developing embryo. The encoded protein participates in cell fate decisions by interacting with the Notch receptor. Loss of function of this gene results in severe defects in neural development and loss of viability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]