Lta4h (NM_008517) Mouse Tagged ORF Clone

CAT#: MG209403

Lta4h (GFP-tagged) - Mouse leukotriene A4 hydrolase (Lta4h)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lta4h
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209403 representing NM_008517
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGAGGTCGCGGATACTTGCTCCTTGGCTTCTCCAGCCTCGGTCTGCCGAACCCAGCACCTCCATC
  TTCGCTGCAGCGTCGACTTCGCTCGCCGGACGCTGACCGGGACGGCCGCGCTCACGGTCCAGTCACAGGA
  GGAGAATCTGCGCAGCCTGACTTTGGACACAAAAGACCTCACAATAGAAAAAGTGGTGATCAATGGACAA
  GAAGTCAAATACACCCTTGGAGAAAGTCAAGGTTACAAAGGGTCACCGATGGAAATCTCTCTTCCCATCG
  CCCTGAGCAAAAATCAAGAGATCGTTATAGAAATTTCCTTTGAGACATCTCCCAAATCCTCTGCGCTTCA
  GTGGCTCACTCCTGAACAGACGTCAGGGAAGCAGCACCCGTATCTCTTCAGTCAGTGCCAGGCCATCCAC
  TGCAGGGCCATCCTCCCTTGCCAAGACACTCCTTCCGTGAAATTAACATACACCGCAGAGGTGTCTGTCC
  CGAAAGAACTGGTGGCTCTCATGAGTGCCATCCGTGATGGAGAAGCCCCTGACCCAGAAGACCCCAGCAG
  GAAGATTTACAGATTCAACCAGAGGGTTCCCATACCATGCTACCTGATTGCTTTGGTTGTTGGAGCTTTA
  GAAAGCAGACAAATTGGCCCAAGAACTCTGGTGTGGTCCGAGAAAGAGCAGGTGGAGAAATCTGCTAATG
  AATTTTCTGAGACCGAATCCATGCTTAAAATTGCAGAAGATCTGGGGGGGCCCTATGTTTGGGGGCAGTA
  CGACCTCTTGGTTCTGCCCCCATCCTTCCCGTACGGTGGCATGGAGAACCCTTGTCTCACGTTCGTAACG
  CCTACGCTACTGGCAGGTGACAAGTCACTCTCCAACGTTATTGCACATGAAATATCTCATAGCTGGACAG
  GAAACCTAGTGACTAACAAGACGTGGGATCACTTTTGGTTAAACGAAGGACATACTGTCTACTTAGAGCG
  CCACATTTGTGGACGGTTGTTTGGGGAAAAGTTCAGACATTTCCATGCTTTGGGAGGATGGGGAGAGCTA
  CAGAATACGATAAAAACATTTGGGGAGTCCCACCCCTTCACCAAGCTCGTGGTTGATCTGAAGGACGTAG
  ACCCCGATGTAGCCTATTCCTCCATCCCCTATGAGAAGGGCTTTGCACTACTCTTCTACCTTGAACAGCT
  TCTTGGGGGACCAGAGGTGTTTCTAGGATTCTTAAAGGCTTATGTTAAGAAGTTTTCCTACCAAAGTGTA
  ACGACTGATGACTGGAAGAGCTTCCTATATTCCCACTTTAAAGACAAGGTGGATCTTCTCAATCAAGTTG
  ATTGGAACGCCTGGCTCTACGCTCCGGGCCTGCCTCCTGTCAAGCCCAATTACGACGTGACTCTGACAAA
  TGCTTGCATTGCCTTAAGTCAAAGATGGGTTACTGCCAAAGAGGAAGATTTAAGCTCATTCAGCATCGCA
  GACCTGAAGGATCTCTCGTCCCATCAGCTGAATGAGTTCCTGGCACAGGTGCTTCAGAAGGCACCTCTTC
  CCTTGGGGCACATAAAGCGAATGCAAGAGGTGTACAACTTCAATGCCATTAACAATTCTGAAATACGATT
  CAGATGGTTACGGCTCTGCATTCAATCGAAATGGGAGGAAGCAATTCCTTTGGCCCTAAAGATGGCAACT
  GAACAAGGAAGGATGAAATTCACACGTCCTCTATTCAAGGATCTCGCTGCCTTTGACAAATCCCATGATC
  AAGCTGTCCACACCTACCAAGAGCATAAAGCCAGCATGCATCCCGTGACTGCGATGCTGGTGGGGAGAGA
  TCTGAAAGTGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209403 representing NM_008517
  Red=Cloning site Green=Tags(s)
  
  MPEVADTCSLASPASVCRTQHLHLRCSVDFARRTLTGTAALTVQSQEENLRSLTLDTKDLTIEKVVINGQ
  EVKYTLGESQGYKGSPMEISLPIALSKNQEIVIEISFETSPKSSALQWLTPEQTSGKQHPYLFSQCQAIH
  CRAILPCQDTPSVKLTYTAEVSVPKELVALMSAIRDGEAPDPEDPSRKIYRFNQRVPIPCYLIALVVGAL
  ESRQIGPRTLVWSEKEQVEKSANEFSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVT
  PTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFHALGGWGEL
  QNTIKTFGESHPFTKLVVDLKDVDPDVAYSSIPYEKGFALLFYLEQLLGGPEVFLGFLKAYVKKFSYQSV
  TTDDWKSFLYSHFKDKVDLLNQVDWNAWLYAPGLPPVKPNYDVTLTNACIALSQRWVTAKEEDLSSFSIA
  DLKDLSSHQLNEFLAQVLQKAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEEAIPLALKMAT
  EQGRMKFTRPLFKDLAAFDKSHDQAVHTYQEHKASMHPVTAMLVGRDLKVD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008517
• ORF Size: 1833 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008517.2, NP_032543.2
• RefSeq Size: 2066
• RefSeq ORF: 1836
• Locus ID: 16993
• Gene Summary: The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]