F2 (NM_010168) Mouse Tagged ORF Clone

CAT#: MG209466

F2 (GFP-tagged) - Mouse coagulation factor II (F2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: F2
• Synonyms: Cf-2; Cf2; FII
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209466 representing NM_010168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCACGTCCGCGGCCTGGGCCTCCCTGGCTGCTTGGCTCTGGCTGCCCTGGTTAGCCTGGTACACA
  GCCAGCATGTGTTCCTGGCGCCTCAGCAAGCACTATCGCTGCTCCAGCGGGTCCGCAGAGCCAACAGTGG
  CTTCCTGGAGGAGCTCCGAAAGGGCAACCTAGAGCGTGAGTGCGTGGAAGAGCAGTGCAGCTATGAGGAG
  GCCTTTGAGGCCCTGGAGTCTCCCCAAGACACAGACGTGTTCTGGGCCAAGTACACAGTGTGTGATTCAG
  TGAGGAAACCTCGAGAGACTTTCATGGACTGTCTGGAAGGTCGCTGTGCTATGGATCTGGGTGTGAACTA
  CCTTGGGACTGTGAATGTCACCCATACCGGTATCCAGTGCCAGCTGTGGCGGAGCCGCTACCCCCACAAG
  CCTGAAATCAACTCCACCACCCATCCTGGGGCAGACCTGAAGGAGAACTTCTGCCGCAATCCAGACAGCA
  GTACCACGGGACCCTGGTGCTACACCACAGATCCTACTGTGCGCAGAGAGGAATGCAGTGTCCCTGTCTG
  TGGCCAGGAGGGCCGTACCACTGTGGTGATGACTCCTCGCTCTGGAGGTTCCAAGGACAATCTGTCACCT
  CCACTGGGACAGTGCCTCACTGAGCGTGGCCGACTATACCAGGGGAATTTGGCTGTGACAACACTTGGGT
  CCCCCTGCCTGCCCTGGAACAGCTTACCAGCCAAGACCCTGAGCAAGTACCAAGACTTCGACCCCGAGGT
  GAAACTCGTGGAAAACTTCTGCCGCAACCCAGACTGGGATGAGGAGGGCGCATGGTGCTATGTTGCGGGG
  CAGCCTGGCGACTTTGAATACTGCAACCTCAACTACTGCGAGGAGGCGGTGGGAGAGGAGAACTATGATG
  TGGACGAGTCCATCGCGGGACGCACCACCGATGCGGAGTTCCACACCTTCTTCAATGAGAAGACCTTCGG
  CCTTGGGGAGGCAGACTGTGGCCTGCGGCCTTTGTTCGAGAAGAAGTCGTTGAAAGACACAACCGAAAAG
  GAGCTTCTTGACTCTTACATAGACGGGCGCATCGTGGAGGGCTGGGACGCTGAGAAGGGTATCGCCCCCT
  GGCAGGTGATGCTTTTTCGGAAGAGTCCCCAAGAGCTGCTGTGTGGGGCCAGCCTTATCAGTGACCGATG
  GGTCCTCACTGCTGCCCACTGCATTCTGTACCCACCCTGGGACAAGAACTTCACTGAGAATGACCTCCTG
  GTGCGCATTGGCAAGCATTCCCGAACCAGATATGAGCGGAATGTTGAAAAGATCTCCATGCTGGAAAAGA
  TCTACGTCCACCCCAGATATAACTGGCGGGAGAACCTAGACCGCGATATCGCTCTGCTCAAGCTAAAGAA
  ACCTGTACCCTTCAGTGACTATATTCACCCCGTGTGTTTGCCAGACAAGCAGACAGTAACCAGCTTGCTC
  CGGGCTGGTTATAAAGGGCGGGTGACAGGCTGGGGCAACCTTCGGGAGACATGGACAACCAACATCAATG
  AGATACAGCCCAGCGTCCTGCAGGTGGTGAACCTGCCCATTGTAGAGCGGCCAGTGTGCAAGGCCTCCAC
  CCGGATTCGAATTACTGACAACATGTTCTGTGCTGGCTTCAAGGTGAATGACACCAAGCGAGGAGATGCT
  TGTGAAGGTGACAGTGGAGGACCTTTTGTCATGAAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGTA
  TTGTCTCATGGGGTGAAGGATGTGACCGGAAGGGGAAATACGGCTTCTACACGCATGTGTTCCGTCTGAA
  AAGGTGGATACAGAAAGTCATTGATCAATTTGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209466 representing NM_010168
  Red=Cloning site Green=Tags(s)
  
  MSHVRGLGLPGCLALAALVSLVHSQHVFLAPQQALSLLQRVRRANSGFLEELRKGNLERECVEEQCSYEE
  AFEALESPQDTDVFWAKYTVCDSVRKPRETFMDCLEGRCAMDLGVNYLGTVNVTHTGIQCQLWRSRYPHK
  PEINSTTHPGADLKENFCRNPDSSTTGPWCYTTDPTVRREECSVPVCGQEGRTTVVMTPRSGGSKDNLSP
  PLGQCLTERGRLYQGNLAVTTLGSPCLPWNSLPAKTLSKYQDFDPEVKLVENFCRNPDWDEEGAWCYVAG
  QPGDFEYCNLNYCEEAVGEENYDVDESIAGRTTDAEFHTFFNEKTFGLGEADCGLRPLFEKKSLKDTTEK
  ELLDSYIDGRIVEGWDAEKGIAPWQVMLFRKSPQELLCGASLISDRWVLTAAHCILYPPWDKNFTENDLL
  VRIGKHSRTRYERNVEKISMLEKIYVHPRYNWRENLDRDIALLKLKKPVPFSDYIHPVCLPDKQTVTSLL
  RAGYKGRVTGWGNLRETWTTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMFCAGFKVNDTKRGDA
  CEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRKGKYGFYTHVFRLKRWIQKVIDQFG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010168
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010168.2, NP_034298.1
• RefSeq Size: 1988
• RefSeq ORF: 1857
• Locus ID: 14061
• Gene Summary: This gene encodes a vitamin K-dependent glycoprotein coagulation factor that plays an important role in the process of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that undergoes enzymatic cleavage by the coagulation factor Xa to form an active serine protease that converts soluble fibrinogen to insoluble fibrin clot. Most of the mice lacking the encoded protein die at an embryonic stage due to defects in yolk sac vasculature, while the rare nenonates succumb to hemorrhage on the first postnatal day. [provided by RefSeq, Apr 2015]