Kars (BC035324) Mouse Tagged ORF Clone

CAT#: MG209505

Kars (GFP-tagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:6923 IMAGE:2811286)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kars
• Synonyms: mKIAA0070
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209505 representing BC035324
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGCTGCTGTCAGGCTTGTTAGGGGGGCCCTGCGCCAAACCTCATGGGCAGAATGGGGTCAGAGGG
  AACTTCGACTGGGCCACCTTGCTCCTTTCACAACGCTCCACAAGGACCAGCCACTTTCTGATAGAAGAAG
  TGAGCTGAAGAGGCGTCTGAAAGCTGAGAAGAAACTGGCAGAGAAGGAGGCCAAGCAGAAAGAGCTGAGT
  GAGAAACAGCTAAACCAGACTGCTTCCGCTCCCAACCACACGGCTGACAATGGCGTGGGTGCTGAGGAGG
  AGACTCTGGACCCAAATCAATACTACAAGATCCGAAGTCAAGCCGTCCAGCAGCTGAAGGTCACTGGGGA
  GGATCCGTACCCACACAAGTTCCACGTGGACATCTCACTCACTCAGTTCATCCAAGAATATAGTCACCTG
  CAGCCTGGGGACCACCTGACTGATGTCACCCTCAAAGTGGCAGGCCGCATCCACGCCAAGAGGGCCTCTG
  GAGGGAAGCTCATCTTCTATGACCTGCGAGGAGAGGGGGTCAAGTTACAAGTCATGGCCAACTCCAGGAA
  TTACAAATCAGAGGAGGAATTTGTTCATATCAATAACAAACTGCGCCGGGGAGACATAATTGGAGTTGAG
  GGCAATCCCGGGAAAACCAAGAAGGGCGAGCTGAGCATCATCCCCCAGGAGATCACGCTGCTGTCCCCCT
  GCTTGCACATGCTGCCTCATCTTCACTTTGGCCTCAAAGACAAGGAAACACGGTATCGTCAAAGATACTT
  GGACTTGATCCTGAACGACTTTGTGAGGCAGAAATTTATCGTCCGCTCTAAGATCATCACATACATAAGA
  AGTTTCTTGGATGAGCTGGGCTTCCTAGAGATCGAAACTCCCATGATGAACATCATTCCCGGGGGAGCTG
  TGGCCAAGCCTTTCATCACCTATCACAATGAGCTGGACATGAACTTGTATATGAGAATTGCTCCAGAACT
  CTACCACAAGATGCTGGTGGTTGGTGGCATTGACCGGGTTTATGAAATTGGGCGCCAGTTCCGGAACGAA
  GGGATTGATTTGACTCACAATCCTGAGTTCACCACCTGTGAGTTCTACATGGCCTATGCAGACTATCATG
  ACCTCATGGAGATCACAGAGAAGATGCTGTCAGGGATGGTGAAGAGCATTACAGGCAGTTACAAGATCAC
  CTACCACCCAGATGGGCCGGAAGGCCAAGCCTACGAGGTCGACTTCACCCCACCCTTCCGAAGAATCAGC
  ATGGTAGAAGAGCTTGAGAAAGCCCTAGGTGTGAAGCTGCCAGAAACCAGTCTCTTTGAAACTGAAGAAA
  CTCGGAAAATTCTTGATGATATTTGTGTTGCAAAAGCTGTTGAATGCCCCCCACCTCGGACCACAGCCAG
  GCTCCTTGATAAGCTCGTTGGCGAGTTCCTCGAAGTCACATGCATCAGCCCTACCTTCATCTGTGATCAC
  CCACAGATCATGAGTCCTTTGGCCAAATGGCACCGCTCCAAAGAGGGTCTCACGGAGCGCTTTGAGCTGT
  TTGTCATGAAGAAGGAGATATGCAATGCCTATACTGAGCTGAATGACCCCGTGCGGCAGAGGCAGCTGTT
  TGAGGAGCAGGCCAAGGCCAAGGCTGCCGGTGATGACGAGGCCATGTTCATAGACGAGAACTTCTGTACT
  GCCCTGGAATACGGGCTGCCTCCCACAGCGGGCTGGGGCATGGGCATTGATCGGCTCACCATGTTTCTCA
  CCGATTCCAACAATATCAAGGAAGTACTTCTGTTTCCTGCCATGAAGCCAGAGGACAAGAAGGAAACTGC
  AGCGACCACTGAAACCCCAGAGAGCACAGAGGCCAGCCCCTCTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209505 representing BC035324
  Red=Cloning site Green=Tags(s)
  
  MQAAVRLVRGALRQTSWAEWGQRELRLGHLAPFTTLHKDQPLSDRRSELKRRLKAEKKLAEKEAKQKELS
  EKQLNQTASAPNHTADNGVGAEEETLDPNQYYKIRSQAVQQLKVTGEDPYPHKFHVDISLTQFIQEYSHL
  QPGDHLTDVTLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFVHINNKLRRGDIIGVE
  GNPGKTKKGELSIIPQEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIVRSKIITYIR
  SFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNE
  GIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVKSITGSYKITYHPDGPEGQAYEVDFTPPFRRIS
  MVEELEKALGVKLPETSLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCISPTFICDH
  PQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPVRQRQLFEEQAKAKAAGDDEAMFIDENFCT
  ALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVLLFPAMKPEDKKETAATTETPESTEASPSV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC035324
• ORF Size: 1869 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC035324, AAH35324
• RefSeq Size: 2146
• RefSeq ORF: 1868
• Locus ID: 85305
• Gene Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. [UniProtKB/Swiss-Prot Function]