Kars (BC027356) Mouse Tagged ORF Clone

CAT#: MG209528

Kars (GFP-tagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kars
• Synonyms: AA589550|AL024334|AL033315|AL033367|D8Ertd698e|D8Wsu108e|LysRS|mKIAA0070
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209528 representing BC027356
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGATGCAAGCTGCTGTCAGGCTTGTTAGGGGGGCCCTGCGCCAAACCTCATGGGCAGAATGGGGTC
  AGAGGGAACTTCGACTGGGCCACCTTGCTCCTTTCACAACGCTCCACAAGGACCAGCCACTTTCTGATAG
  AAGAAGTGAGCTGAAGAGGCGTCTGAAAGCTGAGAAGAAACTGGCAGAGAAGGAGGCCAAGCAGAAAGAG
  CTGAGTGAGAAACAGCTAAACCAGACTGCTTCCGCTCCCAACCACACGGCTGACAATGGCGTGGGTGCTG
  AGGAGGAGACTCTGGACCCAAATCAATACTACAAGATCCGAAGTCAAGCCGTCCAGCAGCTGAAGGTCAC
  TGGGGAGGATCCGTACCCACACAAGTTCCACGTGGACATCTCACTCACTCAGTTCATCCAAGAATATAGT
  CACCTGCAGCCTGGGGACCACCTGACTGATGTCACCCTCAAAGTGGCAGGCCGCATCCACGCCAAGAGGG
  CCTCTGGAGGGAAGCTCATCTTCTATGACCTGCGAGGAGAGGGGGTCAAGTTACAAGTCATGGCCAACTC
  CAGGAATTACAAATCAGAGGAGGAATTTGTTCATATCAATAACAAACTGCGCCGGGGAGACATAATTGGA
  GTTGAGGGCAATCCCGGGAAAACCAAGAAGGGCGAGCTGAGCATCATCCCCCAGGAGATCACGCTGCTGT
  CCCCCTGCTTGCACATGCTGCCTCATCTTCACTTTGGCCTCAAAGACAAGGAAACACGGTATCGTCAAAG
  ATACTTGGACTTGATCCTGAACGACTTTGTGAGGCAGAAATTTATCGTCCGCTCTAAGATCATCACATAC
  ATAAGAAGTTTCTTGGATGAGCTGGGCTTCCTAGAGATCGAAACTCCCATGATGAACATCATTCCCGGGG
  GAGCTGTGGCCAAGCCTTTCATCACCTATCACAATGAGCTGGACATGAACTTGTATATGAGAATTGCTCC
  AGAACTCTACCACAAGATGCTGGTGGTTGGTGGCATTGACCGGGTTTATGAAATTGGGCGCCAGTTCCGG
  AACGAAGGGATTGATTTGACTCACAATCCTGAGTTCACCACCTGTGAGTTCTACATGGCCTATGCAGACT
  ATCATGACCTCATGGAGATCACAGAGAAGATGCTGTCAGGGATGGTGAAGAGCATTACAGGCAGTTACAA
  GATCACCTACCACCCAGATGGGCCGGAAGGCCAAGCCTACGAGGTCGACTTCACCCCACCCTTCCGAAGA
  ATCAGCATGGTAGAAGAGCTTGAGAAAGCCCTAGGTGTGAAGCTGCCAGAAACCAGTCTCTTTGAAACTG
  AAGAAACTCGGAAAATTCTTGATGATATTTGTGTTGCAAAAGCTGTTGAATGCCCCCCACCTCGGACCAC
  AGCCAGGCTCCTTGATAAGCTCGTTGGCGAGTTCCTCGAAGTCACATGCATCAGCCCTACCTTCATCTGT
  GATCACCCACAGATCATGAGTCCTTTGGCCAAATGGCACCGCTCCAAAGAGGGTCTCACGGAGCGCTTTG
  AGCTGTTTGTCATGAAGAAGGAGATATGCAATGCCTATACTGAGCTGAATGACCCCGTGCGGCAGAGGCA
  GCTGTTTGAGGAGCAGGCCAAGGCCAAGGCTGCCGGTGATGACGAGGCCATGTTCATAGACGAGAACTTC
  TGTACTGCCCTGGAATACGGGCTGCCTCCCACAGCGGGCTGGGGCATGGGCATTGATCGGCTCACCATGT
  TTCTCACCGATTCCAACAATATCAAGGAAGTACTTCTGTTTCCTGCCATGAAGCCAGAGGACAAGAAGGA
  AACTGCAGCGACCACTGAAACCCCAGAGAGCACAGAGGCCAGCCCCTCTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209528 representing BC027356
  Red=Cloning site Green=Tags(s)
  
  MLMQAAVRLVRGALRQTSWAEWGQRELRLGHLAPFTTLHKDQPLSDRRSELKRRLKAEKKLAEKEAKQKE
  LSEKQLNQTASAPNHTADNGVGAEEETLDPNQYYKIRSQAVQQLKVTGEDPYPHKFHVDISLTQFIQEYS
  HLQPGDHLTDVTLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFVHINNKLRRGDIIG
  VEGNPGKTKKGELSIIPQEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIVRSKIITY
  IRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFR
  NEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVKSITGSYKITYHPDGPEGQAYEVDFTPPFRR
  ISMVEELEKALGVKLPETSLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCISPTFIC
  DHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPVRQRQLFEEQAKAKAAGDDEAMFIDENF
  CTALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVLLFPAMKPEDKKETAATTETPESTEASPSV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027356
• ORF Size: 1872 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC027356.1
• RefSeq Size: 2205
• RefSeq ORF: 1874
• Locus ID: 85305
• Gene Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. [UniProtKB/Swiss-Prot Function]