Snap91 (BC052454) Mouse Tagged ORF Clone

CAT#: MG209560

Snap91 (GFP-tagged) - Mouse synaptosomal-associated protein 91 (cDNA clone MGC:63436 IMAGE:6410678)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Snap91
• Synonyms: AP180, F1-20, 91kDa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209560 representing BC052454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCCTTGAACAACATCTAAATACCCTAGAAGGAAAGAAACCTGGAAACAATGAAGGATCTGGTG
  CTCCCTCTCCACTAAGTAAGTCTTCTCCAGCCACAACTGTTACATCTCCTAATTCTACACCAGCTAAAAC
  TATCGACACATCCCCGCCAGTTGACATATTTGCAACAGCATCCGCGGCTGCCCCAGTCAGCTCTGCTAAG
  CCATCAAGCGATCTCCTTGATCTTCAGCCCGACTTCTCTGGAGCAGCTGCGGGGGCAGCAGCACCTGTAG
  TGCCTCCTTCTGGGGGTGCGACCGCCTGGGGAGACCTTTTGGGAGAGGATTCCTTGGCTGCACTTTCCTC
  TGTTCCCTGTGAAGCACCGATTTCAGACCCATTTGCACCAGAGCCTTCCCCTCCTACTACAACCACTGAG
  CCTGCTTCAGCCTCTGCCTCGACCACCACAGCTGTGACGGCTGTCACTACGGAAGTGGATCTCTTTGGAG
  ATGCCTTTGCAGCTTCTCCTGGGGAGGCCCCTGCAGCATCCGAAGGGGCTACCGCACCAGCTACCCCGGC
  CCCAGTGGCTGCAGCTCTTGATGCATGCTCAGGAAATGACCCTTTTGCCCCATCTGAAGGTAGCGCAGAG
  GCTGCACCTGAGCTGGACCTCTTTGCAATGAAGCCACCTGAGACCAGCGCTCCTGTAGTTACCCCTACAG
  CTAGCACAGCCCCTCCAGTTCCCGCAACTGCTCCTTCTCCTGCTCCCACGGCTGTGGCAGCCACTGCTGC
  CACCACCACCGCCGCCGCTGCAGCTACCACCACTGCCACCACCTCTGCTGCTGCTGCCACCACCGCCGCT
  GCTCCTCCTGCTCTAGATATCTTTGGTGATTTGTTTGATTCTGCTCCTGAAGTTGCTGCAGCACCTAAGC
  CAGACGCGGCTCCTAGCATAGACCTGTTTGGCACAGATGCTTTCTCCTCCCCGCCACGAGGGGCCTCTCC
  GGTGCCTGAGAGTTCTCTCACTGCTGACCTCTTATCTGTGGACGCATTTGCAGCGCCGTCTCCTGCATCC
  ACTGCCTCTCCTGCAAAGGCGGAGTCCTCGGGTGTCATAGACCTTTTTGGGGATGCGTTTGGAAGTGGTG
  CTTCTGAAACCCAGCCAGCACCACAGGCTGTTTCTAGTTCATCAGCATCGGCAGATCTACTAGCTGGATT
  TGGGGGTTCTTTCATGGCCCCTTCTACAACGCCAGTGACTCCAGCTCAGAATAACCTGCTGCAACCCAGT
  TTCGAGGCAGCTTTTGGAACGACGCCTTCGACTTCAAGCAGCAGCTCTTTTGACCCATCAGTGTTTGATG
  GTTTAGGCGATCTTCTGATGCCAACCATGGCACCATCCGGGCAGCCTGCCCCTGTCTCAATGGTCCCACC
  CAGTCCTGCAATGGCAGCCAGCAAAGGCCTCGGAAGTGACCTTGACTCGTCTCTGGCCAGTTTAGTAGGC
  AATCTTGGGATTTCTGGTACCACATCAAAAAAGGGAGATCTCCAGTGGAATGCTGGGGAGAAAAAGCTGA
  CTGGTGGAGCCAACTGGCAACCGAAAGTCACTCCAGCCACATGGTCAGCGGGTGTTCCCCCGCAAGGCAC
  TGTTCCACCAACCAGCTCAGTCCCTCCGGGTGCCGGGGCCCCGTCCGTTGGGCAACCTGGAGCAGGATTT
  GGAATGCCTCCTTCAGGGACAGGCATGACCATGATGTCTCAGCAGCCAGTCATGTTTGCACAGCCCATGA
  TGAGGCCACCCTTTGGAGCTGCAGCTGTGCCCGGCACACAGCTTTCTCCAAGCCCTACACCTGCCACTCA
  GAGTCCCAAGAAACCTCCAGCCAAGGACCCGTTAGCGGATCTTAACATCAAGGATTTCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209560 representing BC052454
  Red=Cloning site Green=Tags(s)
  
  METLEQHLNTLEGKKPGNNEGSGAPSPLSKSSPATTVTSPNSTPAKTIDTSPPVDIFATASAAAPVSSAK
  PSSDLLDLQPDFSGAAAGAAAPVVPPSGGATAWGDLLGEDSLAALSSVPCEAPISDPFAPEPSPPTTTTE
  PASASASTTTAVTAVTTEVDLFGDAFAASPGEAPAASEGATAPATPAPVAAALDACSGNDPFAPSEGSAE
  AAPELDLFAMKPPETSAPVVTPTASTAPPVPATAPSPAPTAVAATAATTTAAAAATTTATTSAAAATTAA
  APPALDIFGDLFDSAPEVAAAPKPDAAPSIDLFGTDAFSSPPRGASPVPESSLTADLLSVDAFAAPSPAS
  TASPAKAESSGVIDLFGDAFGSGASETQPAPQAVSSSSASADLLAGFGGSFMAPSTTPVTPAQNNLLQPS
  FEAAFGTTPSTSSSSSFDPSVFDGLGDLLMPTMAPSGQPAPVSMVPPSPAMAASKGLGSDLDSSLASLVG
  NLGISGTTSKKGDLQWNAGEKKLTGGANWQPKVTPATWSAGVPPQGTVPPTSSVPPGAGAPSVGQPGAGF
  GMPPSGTGMTMMSQQPVMFAQPMMRPPFGAAAVPGTQLSPSPTPATQSPKKPPAKDPLADLNIKDFL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052454
• ORF Size: 1883 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052454, AAH52454
• RefSeq Size: 4519
• RefSeq ORF: 1883
• Locus ID: 20616
• Gene Summary: Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats. [UniProtKB/Swiss-Prot Function]