Prmt5 (NM_013768) Mouse Tagged ORF Clone

CAT#: MG209659

Prmt5 (GFP-tagged) - Mouse protein arginine N-methyltransferase 5 (Prmt5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prmt5
• Synonyms: Jbp1; Skb1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209659 representing NM_013768
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGATGGCAGTCGGAGGTGCTGGTGGCAGCCGCGTGTCCAGCGGGAGGGACCTGAATTGCGTCC
  CCGAAATAGCTGACACACTGGGTGCTGTGGCCAAGCAGGGGTTTGATTTCCTCTGCATGCCTGTCTTCCA
  CCCGCGTTTCAAGAGGGAGTTCATTCAGGAACCTGCTAAGAATCGGCCTGGCCCCCAGACACGATCAGAC
  CTACTGCTGTCAGGAAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCATCCAGACT
  CAAAAGTGGAGAAGATCCGAAGGAACTCTGAAGCGGCTATGTTACAGGAGCTGAATTTTGGAGCATATCT
  GGGTCTTCCAGCTTTCCTATTGCCCCTTAATCAGGAAGATAACACGAATCTGGCCAGAGTTCTGACCAAC
  CACATCCACACTGGCCACCACTCTTCCATGTTCTGGATGAGGGTACCCTTGGTGGCACCAGAGGACCTGA
  GAGATGATGTAATTGCGAATGCCCCGACTACACACACAGAGGAGTACAGTGGAGAAGAGAAGACATGGAT
  GTGGTGGCATAACTTTCGGACTCTGTGTGACTATAGCAAGAGAATTGCAGTAGCTCTTGAAATTGGAGCT
  GACCTCCCGTCTAATCACGTCATTGACCGCTGGCTTGGAGAGCCCATCAAAGCAGCCATTCTCCCCACCA
  GCATTTTCCTAACCAACAAGAAAGGATTTCCTGTTCTTTCTAAGGTGCAGCAGAGGCTGATCTTCCGGCT
  CCTCAAGTTGGAAGTGCAGTTTATCATCACGGGAACCAACCACCACTCAGAGAAGGAGTTCTGTTCCTAC
  CTCCAGTACTTGGAATACTTAAGCCAAAATCGCCCTCCACCCAATGCCTATGAGCTCTTTGCCAAAGGCT
  ATGAAGACTATCTGCAGTCCCCACTCCAGCCTCTGATGGACAATCTGGAATCTCAGACATATGAAGTGTT
  TGAAAAGGACCCCATCAAATACTCTCAATATCAGCAGGCTATTTATAAATGTTTGCTAGACCGAGTACCA
  GAAGAAGAAAAGGAGACCAATGTCCAGGTACTTATGGTGCTGGGTGCAGGCCGGGGTCCTCTTGTGAATG
  CGTCTCTTCGGGCAGCCAAACAGGCCGAGCGGCGGATCAGGCTGTATGCTGTGGAGAAGAACCCCAATGC
  TGTGGTGACGCTAGAGAACTGGCAGTTTGAAGAATGGGGGAGCCAGGTGACAGTTGTCTCATCAGACATG
  CGGGAATGGGTGGCTCCGGAGAAAGCTGACATTATTGTCAGTGAGCTTCTGGGTTCCTTTGCCGACAACG
  AGCTGTCACCTGAGTGTCTGGATGGAGCACAGCACTTCCTGAAAGATGATGGCGTGAGCATCCCTGGAGA
  ATACACCTCCTTCCTGGCTCCCATTTCTTCCTCTAAGCTGTACAACGAGGTCCGTGCCTGTCGGGAAAAG
  GACCGCGATCCTGAGGCACAGTTTGAGATGCCTTATGTGGTTCGGTTGCACAACTTCCACCAGCTCTCTG
  CTCCTAAGCCCTGCTTTACCTTCAGCCATCCCAACCGAGATCCTATGATTGACAACAACCGCTACTGTAC
  CCTGGAGTTTCCTGTGGAGGTGAACACGGTGCTTCATGGCTTCGCAGGCTACTTTGAGACTGTGCTTTAC
  CGGGACATCACTCTGAGTATCCGCCCAGAGACTCACTCTCCTGGAATGTTCTCATGGTTCCCCATCTTCT
  TCCCCATTAAGCAGCCCATCACGGTGCACGAAGGCCAGAACATCTGTGTGCGTTTCTGGCGATGCAGCAA
  TTCCAAGAAAGTGTGGTACGAGTGGGCGGTGACGGCCCCCGTCTGTTCTTCTATTCACAACCCTACCGGC
  CGCTCCTATACCATTGGCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209659 representing NM_013768
  Red=Cloning site Green=Tags(s)
  
  MAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFIQEPAKNRPGPQTRSD
  LLLSGRDWNTLIVGKLSPWIHPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTN
  HIHTGHHSSMFWMRVPLVAPEDLRDDVIANAPTTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGA
  DLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKVQQRLIFRLLKLEVQFIITGTNHHSEKEFCSY
  LQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVP
  EEEKETNVQVLMVLGAGRGPLVNASLRAAKQAERRIRLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDM
  REWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREK
  DRDPEAQFEMPYVVRLHNFHQLSAPKPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLY
  RDITLSIRPETHSPGMFSWFPIFFPIKQPITVHEGQNICVRFWRCSNSKKVWYEWAVTAPVCSSIHNPTG
  RSYTIGL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013768
• ORF Size: 1911 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013768.1, NP_038796.1
• RefSeq Size: 2328
• RefSeq ORF: 1914
• Locus ID: 27374
• Gene Summary: This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation and the assembly of small nuclear ribonucleoproteins. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]