Edem1 (NM_138677) Mouse Tagged ORF Clone

CAT#: MG209777

Edem1 (GFP-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Edem1
• Synonyms: A130059K23Rik; EDEM; mKIAA0212
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209777 representing NM_138677
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAATGGCGAGCGCTTGTCCTGGGGCTGGTGCTGCTGCGCCTCGGCCTCCACGCGGTGCTCTGGTTGG
  TCTTCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGGTTTCCGCTCAGCTTCGGCTTCCAGCGCTTGAG
  GGACCCCGACGGCTCGGGGCCAGTTGGTCCGCCCGGAGGCCCGGCCTGGCTTCATCGGCCAAGGCGCGGA
  ACGGAGGGGCGGCTTGAGACCCCGCCAGAGCCGGGACCGACGCCGGGACCGGGCGTGTGCGGCCCGGCGC
  ACTGGGGCTATGCCCTGGGCGGCGGTGGCTGCGGCCCGGACGAGTACGAGCGGCGCTACAGCGGCGCCTT
  CCCGCCGCAGCTGCGTGCCCAGATGCGCGACCTGGCGCGGGGCATGTTCGTCTTCGGCTATGACAACTAC
  ATGGCGCACGCCTTTCCGCAGGACGAGCTCAACCCCATCTACTGCCGCGGCCGGGGGCCTGACCGCGGAG
  ACCCTTCCAATCTGAACATCAATGATGTCCTAGGGAATTACTCTCTGACTCTGGTTGATGCCTTGGATAC
  ATTGGCAATAATGGGAAATTCATCCGAGTTCCAGAAGGCAGTCAAGTTAGTGATCAACACTGTTTCATTT
  GACAAAGATTCCACAGTCCAGGTCTTCGAAGCTACGATAAGGGTTCTGGGAAGCCTCCTTTCTGCTCACA
  GAATAATAACTGATTCCAAACAGCCCTTTGGTGACATGACAATTGAGGATTATGATAATGAATTGTTGTA
  CATGGCCCATGACTTGGCTGTGCGGCTCCTTCCAGCCTTTGAAAACACCAAGACAGGGATCCCCTATCCT
  CGGGTGAATCTGAAGACAGGTGTTCCTCCTGACAGCAATAATGAGACCTGCACAGCGGGGGCCGGTTCTC
  TCCTGGTGGAATTTGGGATTCTGAGCCGCCTGCTGGGGGATTCCACTTTTGAGTGGGTTGCCAGACGAGC
  TGTGAAAGCCCTCTGGAACTTGCGGAGCAACGATACAGGATTATTAGGCAATGTTGTGAACATCCAGACA
  GGCCATTGGGTTGGAAAGCAGAGTGGCTTGGGTGCTGGGCTGGATTCCTTCTATGAGTACCTCTTGAAAT
  CTTACATTCTTTTTGGAGAAAAAGAAGATCTAGAGATGTTTAATGCTGCGTATCAGAGCATCCAGAGCTA
  CCTGCGAAGAGGCCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCACCGCTCTACGTCAACGTGAACATG
  TTCAGTGGGCAGCTCATGAACACCTGGATTGACTCACTGCAGGCTTTCTTCCCTGGACTGCAGGTTCTGA
  TAGGGGATGTGGAAGATGCCATCTGCCTACACGCCTTCTACTATGCCATATGGAAGCGGTACGGGGCCCT
  CCCTGAGCGCTATAACTGGCAACTCCAGGCCCCTGATGTTCTCTTCTACCCTCTGAGACCAGAGCTAGTG
  GAGTCCACATATCTCCTCTACCAGGCAACCAAGAATCCCTTCTACCTCCATGTAGGAATGGACATTCTGC
  AGAGTCTTGAAAAATACACAAAAGTCAAATGTGGATATGCTACGCTGCATCACGTCATAGACAAGTCTAA
  AGAAGACCGGATGGAAAGCTTCTTTCTCAGCGAGACTTGCAAATACTTGTATCTGCTATTTGATGAAGAG
  AATCCAGTACACAAATCTGGAACCAGATACATGTTTACAACTGAGGGCCATATCATATCTGTGGACAAAC
  GTCTTCGGGAATTGCCTTGGAAGGAATTCTTCTCTGAAGATGGGGAGCGGGACCAAGAGGAAAAGTTTGT
  ACACAGACCTAAGTCTCAGGAGCTCAGAGTCATTAACTCCAGTTCTAATTGTAATCGTGTTCCTGATGAG
  AGGAGATACTCCTTGCCCTTAAAGAGCATCTACATGCGCCAGATCGACCAGATGGTTGGCTTGATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209777 representing NM_138677
  Red=Cloning site Green=Tags(s)
  
  MQWRALVLGLVLLRLGLHAVLWLVFGLGPSMGFYQRFPLSFGFQRLRDPDGSGPVGPPGGPAWLHRPRRG
  TEGRLETPPEPGPTPGPGVCGPAHWGYALGGGGCGPDEYERRYSGAFPPQLRAQMRDLARGMFVFGYDNY
  MAHAFPQDELNPIYCRGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSF
  DKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIEDYDNELLYMAHDLAVRLLPAFENTKTGIPYP
  RVNLKTGVPPDSNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQT
  GHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQSYLRRGREACNEGEGDPPLYVNVNM
  FSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELV
  ESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSKEDRMESFFLSETCKYLYLLFDEE
  NPVHKSGTRYMFTTEGHIISVDKRLRELPWKEFFSEDGERDQEEKFVHRPKSQELRVINSSSNCNRVPDE
  RRYSLPLKSIYMRQIDQMVGLI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138677
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138677.2, NP_619618.1
• RefSeq Size: 5858
• RefSeq ORF: 1959
• Locus ID: 192193