Pus7 (BC008544) Mouse Tagged ORF Clone

CAT#: MG209845

Pus7 (GFP-tagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (cDNA clone MGC:7703 IMAGE:3497634)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pus7
• Synonyms: C330017I15Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209845 representing BC008544
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATGACAAGCACGTCCCTGAAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCCAC
  ACGACGAAACAAAGAAACAGAAGGTGTCCGAGGGCTGTCTGACCAGCAGTCAGGATGGGGTAGAGAATGA
  CGGTCTACACCGCAGTGAGAACGAGCCTGGGCCTCCAGAAGCTGAAAGCACCGTGAAAGATGACGAGAAT
  TCCAGCGCTCAGGTGCAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGACGGCCTTTCAGAAGCAGGGGAAG
  AGGAAGAAGCAGAGAGTTTTGCAGACATGATGAAACATGGGCTCACAGAACTTGATGTGGGCATCTGCAA
  GTTCGTGAGTTCCCATCATGGCTTCTCAGGGATCTTAAAAGAAAGATACTCTGACTTTGTCGTTCACGAA
  ATCGGGAAAGATGGACGGATCAGCCATTTGGATGACTTATCTGTTCCGGTGGACGAAGAGGACCCTCCAG
  AAGACGCTCTCACTGTCTTGACAGCTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAA
  AGAAACCAGTGTTGCCATCGAGGTCATTGAGGACACCAAAGAGAAGCGAACAGTCATCCATCAAGCCATT
  AAATCTCTCTTCCCAGGACTAGAGACAAAGACAGAGGATAGAGAGGGGCGGAAATACATCGTAGCCTACC
  ATGCAGCTGGCAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCCCGGGGAAGTTACTG
  CCATTTTGTTCTCTACAAGGAAAACAAAGACACCATGGATGCCATCAATGTTCTATCCAAGTACCTGCGG
  GTAAAGCCAAACATATTCTCCTATATGGGAACCAAAGACAAAAGGGCAATCACAGTCCAGGAGATTGCTG
  TTCTCAAAATAAGTGCACAAAGACTTGCCCACCTCAATAAGTGCCTGATGAACTTGAAGCTCGGGAATTT
  CAGCTATCAGAAAACCCCTCTGAAGTTAGGAGCACTCCAAGGAAACCACTTCACTGTTGTTCTCAGGAAT
  ATAACAGGAACTGATGAGCAGGTGCAGCAAGCCATGCAGTCTCTGAGGGAGACCGGCTTCATTAACTACT
  ATGGCATGCAAAGGTTCGGGACCACAGCTGTTCCAACGTACCAGGTTGGAAGAGCAATTCTACAGAATTC
  CTGGACAGAAGTCATGGATTTAATACTGAAACCCCGTTCTGGAGCTGAGAAGGGTTACTTGGTGAAATGC
  AGAGAAGAATGGGCCAAGACCAAAGACCCAGCCTCTGCTCTCAAAAAGCTGCCCGTCAAAAGGTGTGTGG
  AAGGGCAGCTGCTCCGAGGACTTTCCAGATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAATTCC
  AAGAAACAATCGCTTAATGTACATCCACAGTTATCAGAGCTATGTGTGGAACACCATGGTGAGCAGAAGA
  ATTGAGGAGTACGGGCTGAGGCCTGTGCCCGGGGACCTTGTTCTCAAAGGAGCCACGCCCACCTACATTG
  AGGAAGATGACGTTGACAACTACTCCATCCACGATGTGGTGATGCCTTTGCCTGGGTTTGATGTTATCTA
  CCCCAAGCATAAAATCAGTGAGGCCTACAGGGAAATGCTTGCTGCAGACAACCTTGATATTGACAACATG
  AGACACACGATTCGAGATTACTCCTTATCGGGGGCCTACAGAAGGATCATCATCAGGCCTCAGAGCGTCA
  GCTGGGAAGTTGTTGCATATGATGATCCTAAAATTCCACTTTTCAACACGGATGTGGACAACCTCGAGGG
  GAAACCACCACCAGTGTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAGATGGACTTTTCTCTTCCTCCT
  TCTACTTACGCCACCATGGCCATTCGAGAAGTGCTCAAAATGGACACCAGCATCAAGAACCAGACCCAGC
  TAAATACGAGCTGGCTGCGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209845 representing BC008544
  Red=Cloning site Green=Tags(s)
  
  MEMTSTSLKRGCLVVEDNDSVTPHDETKKQKVSEGCLTSSQDGVENDGLHRSENEPGPPEAESTVKDDEN
  SSAQVQEEEEEEEEEDGLSEAGEEEEAESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHE
  IGKDGRISHLDDLSVPVDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVIEDTKEKRTVIHQAI
  KSLFPGLETKTEDREGRKYIVAYHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLR
  VKPNIFSYMGTKDKRAITVQEIAVLKISAQRLAHLNKCLMNLKLGNFSYQKTPLKLGALQGNHFTVVLRN
  ITGTDEQVQQAMQSLRETGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGAEKGYLVKC
  REEWAKTKDPASALKKLPVKRCVEGQLLRGLSRYGMKNIVSAFGIIPRNNRLMYIHSYQSYVWNTMVSRR
  IEEYGLRPVPGDLVLKGATPTYIEEDDVDNYSIHDVVMPLPGFDVIYPKHKISEAYREMLAADNLDIDNM
  RHTIRDYSLSGAYRRIIIRPQSVSWEVVAYDDPKIPLFNTDVDNLEGKPPPVFASEGKYRALKMDFSLPP
  STYATMAIREVLKMDTSIKNQTQLNTSWLR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC008544
• ORF Size: 1982 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC008544, AAH08544
• RefSeq Size: 3137
• RefSeq ORF: 1982
• Locus ID: 78697