Acox1 (NM_015729) Mouse Tagged ORF Clone

CAT#: MG209849

Acox1 (GFP-tagged) - Mouse acyl-Coenzyme A oxidase 1, palmitoyl (Acox1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acox1
• Synonyms: Acox; AOX; D130055E20Rik; Paox
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209849 representing NM_015729
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCCGATCTGCGCAAGGAGCGGGCCGCCGCCACCTTCAATCCAGAGTTAATCACGCACATCTTGG
  ATGGTAGTCCGGAGAACACCCGGCGCCGTCGAGAAATCGAGAACTTGATTCTGAACGATCCAGACTTCCA
  ACATGAGGACTATAACTTCCTCACTCGAAGCCAGCGTTACGAGGTGGCTGTTAAGAAGAGTGCCACCATG
  GTGAAGAAGATGAGGGAATTTGGCATCGCAGACCCTGAAGAAATCATGTGGTTTAAAAACTCTGTGCACC
  GAGGGCATCCTGAGCCTTTGGACCTTCACTTGGGCATGTTCCTGCCCACCTTGCTTCACCAGGCCACCGA
  AGAGCAGCAGGAGCGTTTCTTCATGCCGGCCTGGAATCTGGAGATCACGGGCACTTATGCGCAGACAGAG
  ATGGGTCATGGAACTCATCTTCGAGGCTTGGAAACCACTGCCACATATGACCCCAAGACCCAAGAGTTCA
  TTCTCAACAGCCCAACTGTGACTTCCATCAAGTGGTGGCCTGGGGGGCTTGGGAAGACTTCCAATCATGC
  GATAGTCCTGGCTCAGCTCATCACTCGAGGGGAGTGCTACGGGTTACATGCCTTTGTTGTCCCTATCCGT
  GAGATTGGGACCCACAAGCCTCTGCCAGGCATCACTGTTGGGGATATCGGCCCCAAGTTTGGTTATGAAG
  AGATGGATAATGGCTACCTGAAGATGGACAATTACCGTATTCCTAGAGAGAACATGTTGATGAAATATGC
  CCAGGTGAAGCCTGACGGCACGTATGTAAAACCTCTGAGTAACAAGCTGACATATGGGACCATGGTTTTC
  GTAAGGTCCTTCCTCGTGGGAAGTGCAGCTCAGAGTCTGTCCAAGGCATGCACCATTGCCATTCGATACA
  GTGCTGTGAGGCGCCAGTCTGAAATCAAGAGAAGCGAGCCAGAGCCCCAGATTTTGGATTTTCAGACGCA
  GCAGTATAAACTCTTCCCGCTCCTGGCCACCGCCTATGCCTTCCACTTTCTCGGAAGATACATAAAGGAG
  ACCTACATGCGGATTAATGAGAGCATTGGCCAAGGCGACCTGAGTGAGCTGCCTGAGCTTCATGCCCTCA
  CAGCTGGGCTGAAGGCTTTTACTACCTGGACAGCCAATGCTGGTATCGAAGAATGTCGGATGGCTTGCGG
  TGGGCACGGCTATTCTCACAGCAGTGGGATTCCAAATATTTACGTCACGTTTACCCCGGCCTGCACCTTC
  GAGGGGGAGAACACTGTTATGATGCTGCAGACGGCCAGGTTCTTGATGAAAATCTATGACCAGGTTCAGT
  CGGGGAAGCTGGTGGGTGGTATGGTGTCGTACTTGAATGACCTGCCGAGCCAGCGTATCCAGCCGCAGCA
  GGTGGCAGTCTGGCCAACTCTGGTGGACATTAACAGCCTGGACAGCCTGACAGAAGCCTACAAGCTACGT
  GCAGCCAGATTGGTAGAAATTGCTGCAAAAAACCTTCAGGCCCAAGTGAGTCACAGGAAGAGCAAGGAAG
  TGGCGTGGAACTTGACTTCTGTCGACCTTGTTCGCGCAAGTGAGGCGCACTGCCACTACGTGACCGTTAA
  GGTCTTTGCAGATAAACTCCCCAAGATTCAAGACAGAGCCGTGCAAGCCGTGCTGAGGAACCTGTGTCTC
  TTATATTCTCTCTATGGGATCAGCCAGAAAGGAGGGGATTTTCTTGAGGGGAACATCATCACAGGGGCTC
  AGATGTCACAGGTAAACAGTCGGATCCTGGAGCTGCTCACAGTGACTCGCCCCAACGCTGTGGCTTTGGT
  GGATGCCTTTGACTTTAAGGATGTGACCCTTGGCTCTGTTCTCGGCCGCTATGATGGCAATGTGTATGAA
  AACTTGTTTGAGTGGGCCAAGAAGTCCCCACTGAACAAGACAGAGGTCCACGAATCTTACTACAAGCACT
  TGAAGCCCCTGCAGTCGAAGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209849 representing NM_015729
  Red=Cloning site Green=Tags(s)
  
  MNPDLRKERAAATFNPELITHILDGSPENTRRRREIENLILNDPDFQHEDYNFLTRSQRYEVAVKKSATM
  VKKMREFGIADPEEIMWFKNSVHRGHPEPLDLHLGMFLPTLLHQATEEQQERFFMPAWNLEITGTYAQTE
  MGHGTHLRGLETTATYDPKTQEFILNSPTVTSIKWWPGGLGKTSNHAIVLAQLITRGECYGLHAFVVPIR
  EIGTHKPLPGITVGDIGPKFGYEEMDNGYLKMDNYRIPRENMLMKYAQVKPDGTYVKPLSNKLTYGTMVF
  VRSFLVGSAAQSLSKACTIAIRYSAVRRQSEIKRSEPEPQILDFQTQQYKLFPLLATAYAFHFLGRYIKE
  TYMRINESIGQGDLSELPELHALTAGLKAFTTWTANAGIEECRMACGGHGYSHSSGIPNIYVTFTPACTF
  EGENTVMMLQTARFLMKIYDQVQSGKLVGGMVSYLNDLPSQRIQPQQVAVWPTLVDINSLDSLTEAYKLR
  AARLVEIAAKNLQAQVSHRKSKEVAWNLTSVDLVRASEAHCHYVTVKVFADKLPKIQDRAVQAVLRNLCL
  LYSLYGISQKGGDFLEGNIITGAQMSQVNSRILELLTVTRPNAVALVDAFDFKDVTLGSVLGRYDGNVYE
  NLFEWAKKSPLNKTEVHESYYKHLKPLQSKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015729
• ORF Size: 1983 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015729.2, NP_056544.2
• RefSeq Size: 3992
• RefSeq ORF: 1986
• Locus ID: 11430
• Gene Summary: This gene encodes a member of the acyl-coenzyme A oxidase family. The encoded protein is localized to peroxisomes and is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-coenzyme A to 2-trans-enoyl-coenzyme A. Disruption of this gene results in microvesicular steatohepatitis, spontaneous peroxisome proliferation, and the eventual development of hepatocellular carcinomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]