Clcnkb (BC019983) Mouse Tagged ORF Clone

CAT#: MG209960

Clcnkb (GFP-tagged) - Mouse chloride channel Kb (cDNA clone MGC:28502 IMAGE:4188062)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Clcnkb
• Synonyms: Clc-Ka, CLCKB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209960 representing BC019983
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAATTAGTGGGGCTTCGTGAGGGCGCCTCCAAGAAGCCAGTGCCACTGCAAGAACTCTGGGGGC
  CATGCCCACGTATCCGCAGAAACATCCAGGGGGGCCTAGAGTGGCTGAAAGAGCGGCTCTTCCGTGTGGG
  TGAGGACTGGTACTTCCTGGTGGCTCTCGGGGTGCTCATGGCTCTAATCAGCTATGCTATGAACTTCACT
  ATTGGGCGCGTGGTCAGAGCACACAAGTGGCTGTACAGGGAGATTGGAGACGGCCACTTGCTCCTGTACC
  TCTCCTGGACCGTGTACCCCGTGGCTCTTCTGTCCTTCTCCTCCGGCTTCTCACAGAGCATTACGCCATC
  CTCTGGAGGGTCTGGAATCCCTGAGGTGAAGACCATCTTGACCGGAGTGGTCCTGGAGGACTACCTAGAC
  ATCAAGAACTTTGGGGCCAAGGTGGTGGGCCTCTCCTGCACCCTGGCCACAGGCAGTACCATCTTCCTGG
  GAAAACTGGGCCCCTTCGTGCACCTGAGCGTGATGATCGCTGCTTACCTGGGCCGCGTGCGCACCAAGAC
  CGTCGGGGAACCTGAGAGCAAGACCAAAGAAATGGAACTGTTGGCCGCAGGAGCAGCAGTGGGTGTGGCC
  ACGGTCTTCGCAGCTCCCATCAGCGGTGTCCTGTTCAGCATCGAGGTCATGTCCTCTCACTTCTCCGTCT
  GGGATTACTGGAGGGGCTTCTTCGCTGCCACCTGTGGGGCCTTTATGTTCCACCTCCTGGCGGTCTTCAA
  CAATGAACAGGAGACCATCACCTCCATCTACAAGACCAGCTTTCCAGTGGACATACCCTTTGACTTACCA
  GAGATCTTCTTTTTTGTGGCCCTGGGGGCCATCTGTGGCATCCTGAGCTGCGGGTACAACTACAGCCAGC
  GGACTTTCTTATTTTTCCTCAAGGCCAATGGGTTCACCTCCAAACTGCTGGCTACCAGCAAACCCTTGTA
  CTCAGCCCTGGCTGCTGTGGTCCTGGCTTCCATCACGTATCCCCCTGGTGTGGGCCACTTCATGGCTTCC
  CGGCTGTCCATGTCGGAGCATTTGGAGACACTGTTTGACAACAACTCCTGGGCACTGATGACTAAGAACT
  CGTCTCCACCCTGGGCAGCGGAGCCTGATCCCCAGAAGCTGTGGTTGGAGTGGTGTCACCCACAGTTGAC
  TGTCTTTGGGACACTAGTCTTCTTCCTGGTCATGAAGTTCTGGATGCTGATTTTGGCAACCACCATCCCC
  ATCCCTGCTGGATACTTCTTGCCCATCTTTGTCTATGGAGCTGTCATTGGGCGCCTCTTTGGGGAGGTTC
  TATCTGTCGCCTTCCCAGAGGGCATTGTGGCTGGAGGCAGGGTCAATCCCATCATGCCCGGGGCCTATGC
  TCTGGCAGGTGCTGCTGCCTTCTCGGGGGCGGTGACCCACACCCTCTCCACGGCACTGCTGGCCTTTGAG
  GTAACGGGCCAGCTCGTTCACGCACTGCCTGTGCTGATGGCTGTGCTGGCGGCCAACGCCATCTCTCAGA
  GCTTCCAGCCCTCTTTCTACGATGGCACCATCATTGTCAAGAAACTGCCGTACCTGCCTTGGATCCGTGG
  CCGACAGATTGGTTCCCACTCTGTAACTGTGGGTCACTTCATGAACTGCACCCTCACTACACTGGCCAAG
  GACATGCCCCTGGAGCAAGTGATCCAAGTCATGATCTCCACAGATGTGACCCAGTACCCCCTGGTGGAGA
  CTACAGAGTCTCAGACCCTGGTGGGCGTTGTAAAAAGGACCCACTTGGTGCAAGCCCTCCAGACTGAGCC
  AGCTTCCTGGGCTCCAGGCCAGCAGCCCTATCTCCAGGATATCTTGGCTAATGGCTGCCCCACCCAGCCA
  GTGACACTGCAGCTGTCCCCGGAGACCTCCTTGCATGAGACACACAACCTTTTTGAGCTGCTGAACCTTC
  AGACGCTGTTCGTGACGTCACGAGGCAGAGCTGTGGGCTTTGTATCGTGGGTGGAGGTACTGGGGTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209960 representing BC019983
  Red=Cloning site Green=Tags(s)
  
  MEELVGLREGASKKPVPLQELWGPCPRIRRNIQGGLEWLKERLFRVGEDWYFLVALGVLMALISYAMNFT
  IGRVVRAHKWLYREIGDGHLLLYLSWTVYPVALLSFSSGFSQSITPSSGGSGIPEVKTILTGVVLEDYLD
  IKNFGAKVVGLSCTLATGSTIFLGKLGPFVHLSVMIAAYLGRVRTKTVGEPESKTKEMELLAAGAAVGVA
  TVFAAPISGVLFSIEVMSSHFSVWDYWRGFFAATCGAFMFHLLAVFNNEQETITSIYKTSFPVDIPFDLP
  EIFFFVALGAICGILSCGYNYSQRTFLFFLKANGFTSKLLATSKPLYSALAAVVLASITYPPGVGHFMAS
  RLSMSEHLETLFDNNSWALMTKNSSPPWAAEPDPQKLWLEWCHPQLTVFGTLVFFLVMKFWMLILATTIP
  IPAGYFLPIFVYGAVIGRLFGEVLSVAFPEGIVAGGRVNPIMPGAYALAGAAAFSGAVTHTLSTALLAFE
  VTGQLVHALPVLMAVLAANAISQSFQPSFYDGTIIVKKLPYLPWIRGRQIGSHSVTVGHFMNCTLTTLAK
  DMPLEQVIQVMISTDVTQYPLVETTESQTLVGVVKRTHLVQALQTEPASWAPGQQPYLQDILANGCPTQP
  VTLQLSPETSLHETHNLFELLNLQTLFVTSRGRAVGFVSWVEVLGS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC019983
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC019983.1
• RefSeq Size: 2534
• RefSeq ORF: 2030
• Locus ID: 56365
• Gene Summary: This gene is a member of the CLC family of voltage-gated chloride channels. The gene is located adjacent to a highly similar chloride channel gene on chromosome 4. This gene is syntenic with human CLCNKA (geneID:1187). [provided by RefSeq, Sep 2009]